Central composite design with the help of multivariate curve resolution in loadability optimization of RP‐HPLC to scale‐up a binary mixture

Taheri, MohammadReza; Moazeni-Pourasil, Roudabeh Sadat; Sheikh-Olia-Lavasani, Majid; Karami, Ahmad; Ghassempour, Alireza

doi:10.1002/jssc.201500526

Cited by 9 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, two‐dimensional contour plot is the visual description of the regression equation and assists in the identification of the interactions among selected parameters (Taheri et al . ).…”

Section: Resultsmentioning

confidence: 97%

Optimization of culture conditions for penicilazaphilone C production by a marine-derived fungus Penicillium sclerotiorum M-22

Zhao

Wang

Lin

et al. 2018

Lett Appl Microbiol

View full text Add to dashboard Cite

Penicilazaphilone C (PAC) was a novel azaphilonidal derivative which had exhibited selective cytotoxicity and antibacterial activity. To further enhance production of PAC by optimizing fermentation conditions of Penicillium sclerotiorum M-22 would provide a promising alterative approach for industrial and commercial applications. We used the single factor test to determine the key factors which influence the PAC production. Then through the Response surface methodology and Box-Behnken design to determine the best fermentation condition for maximum production of PAC. Through these experimental designs and analysis will help us improve experimental efficiency and save time and materials.

show abstract

Section: Resultsmentioning

confidence: 97%

Optimization of culture conditions for penicilazaphilone C production by a marine-derived fungus Penicillium sclerotiorum M-22

Zhao

Wang

Lin

et al. 2018

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…This tool helps chromatographers to experimentally optimize methods with a few experimental runs . CCD reducing the number of trials determines optimal values of factors with an upper degree of reliability . There are studies that model isocratic or gradient elution for multicomponent combinations in chromatography by these matrices.…”

Section: Introductionmentioning

confidence: 99%

Response surface methodology based on central composite design accompanied by multivariate curve resolution to model gradient hydrophilic interaction liquid chromatography: Prediction of separation for five major opium alkaloids

Taheri

Bagheri

Moazeni-Pourasil

et al. 2017

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography on bare silica presents some benefits for analysis and purification of ionizable basic alkaloids. This mode was used to separate five major opium alkaloids: morphine, codeine, thebaine, papaverine, and noscapine. Central composite design based on response surface methodology was applied for experimental design, modeling, and optimization in a single-step gradient method. The main effects and their interactions (initial percentage of modifier, changing range of modifier in run time, pH of buffer, and its concentration) were investigated in 30 experiments. Multivariate curve resolution-alternating least squares, by resolving overlapped curves, helped in the accurate calculation of baseline resolution factors to be modeled and optimized more accurately. Then three crucial resolution factors besides elution time were modeled in quadratic and cubic equations and optimized. In addition to the four factors, five extra logarithmic, and nonlogarithmic factors extracted from the four factors to give nine factors overall were inspected on mechanism of retention. It was shown that a linear combination consist of four independence variables successfully describes morphinans retentivity in a single-step gradient method.

show abstract

“…2 However, in practice, the solubility limitation in the mobile phase (MP) causes band touching of the peaks with volume overloading and this does not allow all the stationary phase to work, so that the column diameter determines throughput. To maximize sample loading, touching bands of the two enantiomers takes place automatically.…”

Section: Introductionmentioning

confidence: 99%

“…Since in concentration overloading, throughput is determined by a selectivity factor (α), a high value of α is always recommended. 2 However, in practice, the solubility limitation in the mobile phase (MP) causes band touching of the peaks with volume overloading and this does not allow all the stationary phase to work, so that the column diameter determines throughput. Also, big band broadening causes a long delay between injections.…”

Section: Introductionmentioning

confidence: 99%

Optimization of throughput in semipreparative chiral liquid chromatography using stacked injection

Taheri

Fotovati

Hosseini³

et al. 2017

Chirality

Self Cite

View full text Add to dashboard Cite

An interesting mode of chromatography for preparation of pure enantiomers from pure samples is the method of stacked injection as a pseudocontinuous procedure. Maximum throughput and minimal production costs can be achieved by the use of total chiral column length in this mode of chromatography. To maximize sample loading, often touching bands of the two enantiomers is automatically achieved. Conventional equations show direct correlation between touching-band loadability and the selectivity factor of two enantiomers. The important question for one who wants to obtain the highest throughput is "How to optimize different factors including selectivity, resolution, run time, and loading of the sample in order to save time without missing the touching-band resolution?" To answer this question, tramadol and propranolol were separated on cellulose 3,5-dimethyl phenyl carbamate, as two pure racemic mixtures with low and high solubilities in mobile phase, respectively. The mobile phase composition consisted of n-hexane solvent with alcohol modifier and diethylamine as the additive. A response surface methodology based on central composite design was used to optimize separation factors against the main responses. According to the stacked injection properties, two processes were investigated for maximizing throughput: one with a poorly soluble and another with a highly soluble racemic mixture. For each case, different optimization possibilities were inspected. It was revealed that resolution is a crucial response for separations of this kind. Peak area and run time are two critical parameters in optimization of stacked injection for binary mixtures which have low solubility in the mobile phase.

show abstract

Central composite design with the help of multivariate curve resolution in loadability optimization of RP‐HPLC to scale‐up a binary mixture

Cited by 9 publications

References 24 publications

Optimization of culture conditions for penicilazaphilone C production by a marine-derived fungus Penicillium sclerotiorum M-22

Optimization of culture conditions for penicilazaphilone C production by a marine-derived fungus Penicillium sclerotiorum M-22

Response surface methodology based on central composite design accompanied by multivariate curve resolution to model gradient hydrophilic interaction liquid chromatography: Prediction of separation for five major opium alkaloids

Optimization of throughput in semipreparative chiral liquid chromatography using stacked injection

Contact Info

Product

Resources

About