Central connectivity of the chorda tympani afferent terminals in the rat rostral nucleus of the solitary tract

Park, Sook Kyung; Lee, Dae Seop; Bae, Jin Young; Bae, Yong Chul

doi:10.1007/s00429-014-0959-6

Cited by 9 publications

(8 citation statements)

References 78 publications

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“…Sections were immunostained for GABA and glycine (Gly) by the postembedding immunogold staining method, as previously described from our laboratory (Paik et al, ; Park, Lee, Bae, & Bae, ). Briefly, the grids were treated for 15 min in 1% periodic acid, and for 10 min in 9% sodium periodate, transferred to tris‐buffered saline containing 0.1% triton X‐100 (TBST; pH 7.4) and incubated in 2% human serum albumin (HSA) in TBST for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…These antisera (a kind gift from professor O.P. Ottersen at the Center for Molecular Biology and Neuroscience, University of Oslo) have been extensively characterized (Takumi, Ramírez‐León, Laake, Rinvik, & Ottersen, ) and routinely used in our previous works (Bae et al, ; Paik et al, ; Park et al, , ). Their specificity was also confirmed on thin sections of “sandwiches” of rat brain macromolecule‐glutaraldehyde complexes of different amino acids, including GABA, glutamate, taurine, glycine, aspartate, glutamine, and none (Bae et al, ; Ottersen, ).…”

Section: Methodsmentioning

confidence: 99%

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Synaptic connectivity of urinary bladder afferents in the rat superficial dorsal horn and spinal parasympathetic nucleus

Park

Devi

Bae

et al. 2019

J of Comparative Neurology

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That visceral sensory afferents are functionally distinct from their somatic analogues has been known for a long time but the detailed knowledge of their synaptic connections and neurotransmitters at the first relay nucleus in the spinal cord has been limited. To provide information on these topics, we investigated the synapses and neurotransmitters of identified afferents from the urinary bladder to the superficial laminae of the rat spinal dorsal horn (DH) and the spinal parasympathetic nucleus (SPN) by tracing with horseradish peroxidase, quantitative electron microscopical analysis, and immunogold staining for GABA and glycine. In the DH, most bladder afferent boutons formed synapses with 1-2 postsynaptic dendrites, whereas in the SPN, close to a half of them formed synapses with 3-8 postsynaptic dendrites. The number of postsynaptic dendrites and dendritic spines per bladder afferent bouton, both measures of synaptic divergence and of potential for synaptic plasticity at a single bouton level, were significantly higher in the SPN than in the DH. Bladder afferent boutons frequently received inhibitory axoaxonic synapses from presynaptic endings in the DH but rarely in the SPN. The presynaptic endings were GABA-and/or glycineimmunopositive. The bouton volume, mitochondrial volume, and active zone area, all determinants of synaptic strength, of the bladder afferent boutons were positively correlated with the number of postsynaptic dendrites. These findings suggest that visceral sensory information conveyed via the urinary bladder afferents is processed differently in the DH than in the SPN, and differently from the way somatosensory information is processed in the spinal cord.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Synaptic connectivity of urinary bladder afferents in the rat superficial dorsal horn and spinal parasympathetic nucleus

Park

Devi

Bae

et al. 2019

J of Comparative Neurology

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“…Recently, we showed that the terminals of chorda tympani afferents, primarily an Aδ fiber, convey gustatory information from the anterior part of the tongue and make simple synaptic connections in the first relay nucleus, the rNST. This organization differs from that of the complex synaptic connections made by non-gustatory Aδ fibers in the trigeminal nuclei ( Alvarez et al, 1992 ; Park et al, 2016 ). However, less is yet known about the central connectivity of the glutamatergic rNST neurons in the PBN and RF.…”

Section: Introductionmentioning

confidence: 76%

Ultrastructure of Rat Rostral Nucleus of the Solitary Tract Terminals in the Parabrachial Nucleus and Medullary Reticular Formation

Park

Cho

Kim

et al. 2022

Front. Cell. Neurosci.

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Neurons in the rostral nucleus of the solitary tract (rNST) receive taste information from the tongue and relay it mainly to the parabrachial nucleus (PBN) and the medullary reticular formation (RF) through two functionally different neural circuits. To help understand how the information from the rNST neurons is transmitted within these brainstem relay nuclei in the taste pathway, we examined the terminals of the rNST neurons in the PBN and RF by use of anterograde horseradish peroxidase (HRP) labeling, postembedding immunogold staining for glutamate, serial section electron microscopy, and quantitative analysis. Most of the anterogradely labeled, glutamate-immunopositive axon terminals made a synaptic contact with only a single postsynaptic element in PBN and RF, suggesting that the sensory information from rNST neurons, at the individual terminal level, is not passed to multiple target cells. Labeled terminals were usually presynaptic to distal dendritic shafts in both target nuclei. However, the frequency of labeled terminals that contacted dendritic spines was significantly higher in the PBN than in the RF, and the frequency of labeled terminals that contacted somata or proximal dendrites was significantly higher in the RF than in the PBN. Labeled terminals receiving axoaxonic synapses, which are a morphological substrate for presynaptic modulation frequently found in primary sensory afferents, were not observed. These findings suggest that the sensory information from rNST neurons is processed in a relatively simple manner in both PBN and RF, but in a distinctly different manner in the PBN as opposed to the RF.

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“…The bouton volume, mitochondrial volume and active zone area of the VGLUT2+ boutons on the JC γ-motoneurons, all measures of synaptic strength, were uniformly small and similar to those of VGLUT1+ and VGLUT2+ boutons on the JO and JC α-motoneurons that originate from intrinsic premotoneurons [6], and different from those of VGLUT1+ boutons of the Vmes afferents [6] or primary sensory afferents, which are mostly large [6, 20, 21]. We interpret this to suggest that the glutamatergic input originating from intrinsic premotoneurons exerts a much weaker influence on the JC γ-motoneurons (as well as on JC and JO α-motoneurons) than glutamatergic input originating from proprioceptive primary afferents, and it may be involved in the fine regulation of isometric contraction of the JC muscles.…”

Section: Resultsmentioning

confidence: 99%

Vesicular Glutamate Transporter 1 (VGLUT1)- and VGLUT2-containing Terminals on the Rat Jaw-closing γ-Motoneurons

et al. 2019

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Currently, compared to jaw-closing (JC) α-motoneurons, the information on the distribution and morphology of glutamatergic synapses on the jaw-closing (JC) γ-motoneurons, which may help elucidate the mechanism of isometric contraction of the JC muscle, is very limited. This study investigated the distribution and ultrastructural features of vesicular glutamate transporter 1 (VGLUT1)- and VGLUT2-immunopositive (+) axon terminals (boutons) on JC γ-motoneurons by retrograde tracing with horseradish peroxidase, electron microscopic immunocytochemistry, and quantitative analysis. About 35% of the boutons on identified JC γ-motoneurons were VGLUT+, and of those, 99% were VGLUT2+. The fraction of VGLUT1+ boutons of all boutons and the percentage of membrane of JC γ-motoneurons covered by these boutons were significantly lower than those for the JC α-motoneurons, revealed in our previous work. The bouton volume, mitochondrial volume, and active zone area of the VGLUT2+ boutons on the JC γ-motoneurons were uniformly small. These findings suggest that the JC γ-motoneurons, in contrast to the JC α-motoneurons, receive generally weak glutamatergic synaptic input almost exclusively from VGLUT2+ premotoneurons that form direct synapse with motoneurons.

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Central connectivity of the chorda tympani afferent terminals in the rat rostral nucleus of the solitary tract

Cited by 9 publications

References 78 publications

Synaptic connectivity of urinary bladder afferents in the rat superficial dorsal horn and spinal parasympathetic nucleus

Synaptic connectivity of urinary bladder afferents in the rat superficial dorsal horn and spinal parasympathetic nucleus

Ultrastructure of Rat Rostral Nucleus of the Solitary Tract Terminals in the Parabrachial Nucleus and Medullary Reticular Formation

Vesicular Glutamate Transporter 1 (VGLUT1)- and VGLUT2-containing Terminals on the Rat Jaw-closing γ-Motoneurons

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