Central European Strigeidae Railliet, 1919 (Trematoda: Strigeidida): Molecular and comparative morphological analysis suggests the reclassification of Parastrigea robusta Szidat, 1928 into Strigea Abildgaard, 1790

Heneberg, Petr; Sitko, Jiljí; Těšínský, Miroslav; Rząd, Izabella; Bizos, Jiří

doi:10.1016/j.parint.2018.07.003

Cited by 26 publications

(51 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results reveal the unclear taxonomic position and composite structure of C. syrius and C. cornutus, as reported previously by Heneberg et al [13]. According to these authors, trematode specimens morphologically identi ed as C. syrius and sampled from the typical host, the mute swan Cygnus olor, represent two distinct molecular lineages: one recognized as the typical C. syrius sensu stricto, and the other, according to Heneberg et al [13], as a C. cornutus-like isolate due to its similarity to sequences obtained from adult specimens of C. cornutus sampled from Eurasian teal Anas crecca.…”

Section: Discussionsupporting

confidence: 83%

“…Despite the recognized role of leeches in the transmission of some digenean parasites in aquatic ecosystems there is insu cient understanding of host-parasite relationships within this group of annelids, and only a few papers have analysed various taxonomic (e.g., [3,4,5,6]) and ecological (e.g., [7,8,9,10,11]) aspects of the occurrence and diversity of digenean larval stages in leeches. Moreover, contemporary understanding of the diversity of Strigeidae trematodes has been signi cantly changed by the recently discovered high level of interspeci c and intergeneric homogeneity of morphological features related to an unexpectedly high level of genetic diversity, revealed within the genera Australapatemon and Cotylurus [12,13,14,15]. Regarding these facts, several various aspects of the ecology, taxonomy and phylogeny of the genera Australapatemon and Cotylurus require urgent, detailed studies.…”

Section: Introductionmentioning

confidence: 99%

“…This led to basic problems with precise and adequate delimiting of particular species, which clearly indicates the need to use molecular techniques in studies on the taxonomy and phylogeny of Cotylurus. In recent years, Heneberg et al [13] has combined a morphological and molecular analysis of central European Strigeidae from avian de nitive hosts to investigate the taxonomic position of several Cotylurus species and surprisingly revealed a high level of molecular diversity within morphologically wellestablished species. Locke et al [31] and Gordy and Hanington [14] also revealed the occurrence of several new species within this genus in Canada.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Leeches as the intermediate host for strigeid trematodes – genetic diversity and taxonomy of the genera Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928

Pyrka

Kanarek

Zaleśny

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundLeeches (Hirudinida) play a significant role as intermediate hosts in the circulation of trematodes in the aquatic environment. However, the species richness, molecular diversity and phylogeny of larval stages of Strigeidae trematodes (tetracotyle) occurring in this group of aquatic invertebrates remain poorly understood. In the present work, on the basis of recently obtained sequences of several molecular markers we analysed several aspects of the ecology, taxonomy and phylogeny of the genera Australapatemon and Cotylurus, which utilise leeches as intermediate hosts.MethodsFrom April 2017 to September 2018, 153 leeches were collected from several sampling stations in small rivers with slow-flowing waters and related drainage canals located in three regions of Poland. The distinctive forms of tetracotyle metacercariae collected from leeches supplemented with adult Strigeidae specimens sampled from a wide range of water birds were analysed using the 28S rDNA partial gene, ITS2 region and the COI fragment.ResultsAmong investigated leeches, metacercariae of the tetracotyle type were detected in the parenchyma and musculature of 62 specimens (prevalence 40.5%) with a mean intensity reaching 19.9 ind. The taxonomic generic affiliation of metacercariae derived from leeches revealed the occurrence of two Strigeidae genera: Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928. Phylogenetic reconstructions based on the partial 28S rRNA gene, ITS2 region and partial COI gene confirmed the separation of the Australapatemon and Cotylurus clades. Unfortunately, regarding currently available molecular data and our results, it is not possible to precisely define the taxonomic position of the recently sequenced tetracotyle of Australapatemon. On the other hand, on the basis of the obtained sequences, supplemented with previously published data, the metacercariae of Cotylurus detected in leeches were identified as two species: C. strigeoides Dubois, 1958 and C. syrius Dubois, 1934. This is the first record of C. syrius from the intermediate host.ConclusionsThe results suggest the separation of ecological niches and life cycles between C. cornutus (Rudolphi, 1808) and C. strigeoides/C. syrius with potential serious evolutionary consequences for a wide range of host–parasite relationships. Moreover, phylogenetic analyses corroborated the polyphyletic character of C. syrius, the unclear status of C. cornutus and the separate position of Cotylurs raabei Bezubik, 1958 within Cotylurus. The data demonstrate the inconsistent and confusing taxonomic status of the sequenced tetracotyle of Australapatemon, resulting, in our opinion, from the limited availability of fully reliable, comparative sequences of related taxa in GenBank.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Leeches as the intermediate host for strigeid trematodes – genetic diversity and taxonomy of the genera Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928

Pyrka

Kanarek

Zaleśny

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In Europe, ducks are the definitive hosts of S. robusta. This species was reported to occur in 3% of Anas platyrhynchos and 0.9% of Aythya fuligula [44]. According to Sudarikov [45], a wider spectrum of birds can be infected with S. robusta.…”

Section: Discussionmentioning

confidence: 99%

Strigea robusta causes polydactyly and severe forms of Rostand’s anomaly P in water frogs

et al. 2020

View full text Add to dashboard Cite

Background: Cases of polydactyly in natural populations of amphibians have attracted great interest from biologists. At the end of the 1940s, the French biologist Jean Rostand discovered a polymorphic syndrome in some water frog (Anura: Pelophylax) populations that included polydactyly and some severe morphological anomalies (he called it 'anomaly P'). The cause of this anomaly remains unknown for 70 years. In a previous study, we obtained anomaly P in the laboratory in tadpoles of water frogs that developed together with molluscs Planorbarius corneus (Mollusca: Gastropoda) collected in the field. We thus proposed the 'trematode hypothesis' , according to which the infectious agent responsible for anomaly P is a trematode species. Methods: Metacercariae from tadpoles with anomaly P were identified using ITS2 gene sequencing as Strigea robusta (Trematoda: Strigeidae). To verify teratogenic features of the species, cercariae of S. robusta were tested for the possibility to cause anomalies. Identification of cercariae species was made using morphological and molecular methods (sequencing of ITS2 and 28S rRNA). The tadpoles were exposed to parasites at four doses of cercariae (control, low, medium and high) and divided into two groups: "early" (at 25-27 Gosner stages) and "late" (at 29-34 Gosner stages) exposure. Results: A total of 58 (72.5%) tadpoles survived until metamorphosis under the dose-dependent experiment with the trematode S. robusta. Differences in the survival rates were observed between the exposed and unexposed tadpoles both in the group of "early" tadpoles and "late" tadpoles. The exposure of tadpoles to the cercariae of S. robusta induced anomaly P in 82% of surviving tadpoles. The severe forms developed only in "early" stages under all doses of cercariae exposure. Polydactyly predominantly developed in the "late" stages; under a light exposure dose, polydactyly also developed in "early" tadpoles. Laboratory-hatched tadpoles reared together with infected snails had different rates of survival and complexity of deformations associated with the period of coexistence. Conclusions: The experiments with direct cercariae exposure provide compelling evidence that S. robusta leads to anomaly P in tadpoles of water frogs. The manifestation of anomaly P turned out to be dependent on the stage of development, cercariae dose, and the location of the cysts.

show abstract

“…For molecular examination, we extracted, amplified and sequenced the DNA using the primers that targeted the nuclear ribosomal DNA ( 18S -ITS1– 5.8S- ITS2 and 28S rDNA) and three mitochondrial loci ( cox 1, nad 1 and 12S rDNA) as described [53]. The primers used are specified in Additional file 14: Table S2.…”

Section: Methodsmentioning

confidence: 99%

Massive infection of a song thrush by Mesocestoides sp. (Cestoda) tetrathyridia that genetically match acephalic metacestodes causing lethal peritoneal larval cestodiasis in domesticated mammals

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background Peritoneal larval cestodiasis induced by Mesocestoides Vaillant, 1863 (Cyclophyllidea: Mesocestoididae) is a common cause of severe infections in domestic dogs and cats, reported also from other mammals and less frequently from birds. However, there is a limited knowledge on the taxonomy of causative agents of this disease. Results In the present study, we investigated a massive, likely lethal, infection of a song thrush Turdus philomelos (Passeriformes: Turdidae) by Mesocestoides sp. tetrathyridia. We performed combined morphological and phylogenetic analysis of the tetrathyridia and compared them with the materials obtained previously from other birds and mammals. The metrical data fitted within the wide range reported by previous authors but confirmed the limited value of morphological data for species identification of tetrathyridia of Mesocestoides spp. The molecular analyses suggested that the isolates represented an unidentified Mesocestoides sp. that was previously repeatedly isolated and sequenced in larval and adult forms from domestic dogs and cats in Europe, the Middle East and North Africa. In contrast to the present study, which found encysted tetrathyridia, four of the five previous studies that identified the same species described infections by acephalic metacestodes only. Conclusions The tetrathyridia of the examined Mesocestoides sp. are described in the present study for the first time. However, the possible match with the species that were previously reported to infect birds remains uncertain. The phylogenetic analyses also suggested the rejection of two cases that were previously identified as Mesocestoides corti as they were likely caused by the same species as in the presently reported infection case. The newly provided DNA sequences should allow the assignment to species in the future, when adults of the genus Mesocestoides are more thoroughly sequenced. Electronic supplementary material The online version of this article (10.1186/s13071-019-3480-1) contains supplementary material, which is available to authorized users.

show abstract

Central European Strigeidae Railliet, 1919 (Trematoda: Strigeidida): Molecular and comparative morphological analysis suggests the reclassification of Parastrigea robusta Szidat, 1928 into Strigea Abildgaard, 1790

Cited by 26 publications

References 23 publications

Leeches as the intermediate host for strigeid trematodes – genetic diversity and taxonomy of the genera Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928

Leeches as the intermediate host for strigeid trematodes – genetic diversity and taxonomy of the genera Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928

Strigea robusta causes polydactyly and severe forms of Rostand’s anomaly P in water frogs

Massive infection of a song thrush by Mesocestoides sp. (Cestoda) tetrathyridia that genetically match acephalic metacestodes causing lethal peritoneal larval cestodiasis in domesticated mammals

Contact Info

Product

Resources

About