Central Mechanisms of Gastric Electrical Stimulation Involving Neurons in the Paraventricular Nucleus of the Hypothalamus in Rats

Tang, Ming; Zhang, Jing; Chen, J D Z

doi:10.1381/096089206776116372

Cited by 45 publications

(34 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggested that both the frequency and width of the stimulation pulse play an important role in the modulation of thoracic spinal neuronal activity. In general, results are similar to observations on the effects of GES on neuronal activity in the nucleus of the solitary tract and in the paraventricular nucleus of hypothalamus in rats (Qin et al 2005;Tang et al 2006). …”

Section: Effects Of Gessupporting

confidence: 88%

“…In the present study, we used four sets of stimulation parameters and the selection of these parameters was based on the following: Parameter A is the set of parameters commonly used for the treatment of obesity (D'Argent et al 2002); in parameter B, the frequency is reduced to 14 Hz which is commonly used in treating nausea and vomiting in patients with gastroparesis (Abell et al and Forster et al 2002); Parameter C is similar to Parameter A except that the pulse width is increased to 3ms. The reason behind this selection is that this set of parameters was known more effective in activating GD-responsive neurons in the paraventricular nucleus (Tang et al 2006). Parameter D is typically used to alter gastric motility functions (Ouyang et al 2005).…”

Section: Effects Of Gesmentioning

confidence: 99%

See 1 more Smart Citation

Modulatory effects and afferent pathways of gastric electrical stimulation on rat thoracic spinal neurons receiving input from the stomach

Qin

Chen

Zhang

et al. 2007

Neuroscience Research

Self Cite

View full text Add to dashboard Cite

Gastric electrical stimulation (GES) has been suggested as a potential therapy for patients with obesity or gastric motility disorders. The aim of this study was to investigate the spinal mechanism of GES effects on gastric functions. Extracellular potentials of single spinal (T9-T10) neurons were recorded in pentobarbital anesthetized, paralyzed, ventilated male rats (n=19). Gastric distension (GD) was produced by air inflation of a balloon. One pair of platinum electrodes (1.0-1.5 cm apart) was sutured onto the serosal surface of the lesser curvature of the stomach. GES with four sets of parameters was applied for one minute: GES-A (6 mA, 0.3 ms, 40 Hz, 2s on, 3s off), GES-B (6 mA, 0.3 ms, 14 Hz, 0.1s on, 5s off), GES-C (6 mA, 3 ms, 40 Hz, 2s on, 3s off), GES-D (6 mA, 200 ms, 12 pulses/min). 62/158 (39%) spinal neurons responded to GD (20, 40, 60 mmHg, 20s. Most GD-responsive neurons (n=43) had excitatory responses; the remainder had inhibitory (n=12) or biphasic responses (n=7). GES-A, -B, -C and -D affected activity of 12/33 (36%), 4/31 (13%), 22/29 (76%) and 13/30 (43%) GD-responsive neurons, respectively. Bilateral cervical vagotomy did not significantly alter mean excitatory neuronal responses to GD (n=5) or GES (n=6). Resiniferatoxin (2.0 μg/kg, i.v.), an ultrapotent agonist of vanilloid receptor-1, abolished excitatory responses to GD and GES in 4/4 neurons recorded in vagotomized rats. The results suggested that GES mainly had an excitatory effect on T9-T10 spinal neurons with gastric inputs; neuronal responses to GES were strengthened with stimulation at an increased pulse width and/or number of pulses. The modulatory effect of GES involved thoracic spinal (sympathetic) afferent fibers containing vanilloid receptor-1.

show abstract

Section: Effects Of Gessupporting

confidence: 88%

Section: Effects Of Gesmentioning

confidence: 99%

Modulatory effects and afferent pathways of gastric electrical stimulation on rat thoracic spinal neurons receiving input from the stomach

Qin

Chen

Zhang

et al. 2007

Neuroscience Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…These results suggested that both the frequency and width of the stimulation pulse play an important role in the modulation of thoracic spinal neuronal activity. In general, these data are similar to previous observations on the effects of GES on the activity of thoracic spinal neurons with gastric input (Qin et al 2007), and on neuronal activity in the nucleus of the solitary tract and in the paraventricular nucleus of the hypothalamus in rats (Qin et al 2005;Tang et al 2006). Because a large overlap of spinal innervation for the stomach and duodenum occurs in the lower thoracic segments (Hazarika et al 1964;Khurana and Petras 1991), it is likely that spinal afferent impulses evoked by GES activated spinal neurons that also received primary input from the duodenum.…”

Section: Effects Of Gessupporting

confidence: 91%

Characterization of T9–T10 spinal neurons with duodenal input and modulation by gastric electrical stimulation in rats

et al. 2007

Self Cite

View full text Add to dashboard Cite

Gastric electrical stimulation (GES) has been suggested as a therapy for patients with gastric motility disorders or morbid obesity. However, it is unclear whether GES also affects intestinal sensory and motor functions. Furthermore, little is known about intraspinal visceroreceptive transmission and processing for duodenal afferent information. The aims of this study were to characterize responses of thoracic spinal neurons to duodenal distension, to determine the afferent pathway, and to examine the effects of GES on activity of these neurons. Extracellular potentials of single T9-T10 spinal neurons were recorded in pentobarbital anesthetized, paralyzed, ventilated male rats (n=19). Graded duodenal distension (DD, 0.2-0.6 ml, 20 s) was produced by water inflation of a latex balloon surgically placed into the duodenum. One pair of platinum electrodes (1.0-1.5 cm apart) was sutured onto the serosal surface of the lesser curvature of the stomach. GES with four sets of parameters was applied for one minute: GES-A (6 mA, 0.3 ms, 40 Hz, 2s on, 3s off), GES-B (6 mA, 0.3 ms, 14 Hz, 0.1s on, 5s off), GES-C (6 mA, 3 ms, 40 Hz, 2s on, 3s off), GES-D (6 mA, 200 ms, 12 pulses/min). Results showed that 33/117 (28%) spinal neurons responded to noxious DD (0.4 ml, 20s). Of these, 7 (6%) neurons had low-threshold responses to DD (≤0.2 ml) and 26 (22%) had high-threshold responses to DD (≥0.4 ml). DD-responsive spinal neurons were encountered more frequently in deeper (depth: 0.3-1.2 mm) than in superficial laminae (depth: <0.3 mm) of the dorsal horn (24/67 vs 9/50, P<0.05). DD excited all 9 superficial neurons. In contrast, 20 deeper neurons were excited and 4 neurons were inhibited by DD. Activity of DD-responsive neurons was affected more frequently with GES-C (13/15, 87%) than GES-A (6/16, 38%), -B (3/15, 20%), -D (5/14, 36%), (P<0.01). Bilateral cervical vagotomy did not significantly alter the effects of DD and GES on 5/5 neurons. Resiniferatoxin (2.0 μg/kg, i.v.), an ultrapotent agonist of transient receptor potential vanilloid receptor-1 (TRPV1), abolished DD responses and GES effects on all neurons examined in vagotomized rats. Additionally, 29/33 (88%) DD-responsive neurons received inputs from somatic receptive fields on the back, flank, and medial/lateral abdominal areas. It was concluded that GES mainly exerted an excitatory effect on T9-T10 spinal neurons with duodenal input transmitted by sympathetic afferent fibers expressing TRPV1; spinal neuronal responses to GES were strengthened with an increased pulse width and/or frequency of stimulation; T9-T10 spinal neurons processed input from the duodenum and might mediate effects of GES on duodenal sensation and motility.

show abstract

“…60 All three methods of GES (short pulse, long pulse and trains of short pulses) were able to activate these neurones. However, in one specific type of neurones (gastric distention-inhibitory neurones), opposite effects were noted between GES with trains of short pulses (with parameters used for treating obesity) and GES of short pulses (with parameters used for treating nausea and vomiting in patients with gastroparesis).…”

Section: Central Neuronal Effectsmentioning

confidence: 91%

Systematic review: applications and future of gastric electrical stimulation

Zhang

Chen

2006

Aliment Pharmacol Ther

106

114

View full text Add to dashboard Cite

show abstract

Central Mechanisms of Gastric Electrical Stimulation Involving Neurons in the Paraventricular Nucleus of the Hypothalamus in Rats

Abstract: GES activates gastric-related neurons in the PVN, and the excitatory effect of GES seems related to stimulation strength. The GES used for treating gastroparesis and the GES used for treating obesity seem to solicit different neuronal responses in the PVN.

Cited by 45 publications

References 32 publications

Modulatory effects and afferent pathways of gastric electrical stimulation on rat thoracic spinal neurons receiving input from the stomach

Modulatory effects and afferent pathways of gastric electrical stimulation on rat thoracic spinal neurons receiving input from the stomach

Characterization of T9–T10 spinal neurons with duodenal input and modulation by gastric electrical stimulation in rats

Systematic review: applications and future of gastric electrical stimulation

Contact Info

Product

Resources

About