Central neural circuitry mediating courtship song perception in male Drosophila

Zhou, Chuan; Franconville, Romain; Vaughan, Alexander; Robinett, Carmen C.; Jayaraman, Vivek; Baker, Bruce S.

doi:10.7554/elife.08477

Cited by 77 publications

(105 citation statements)

References 67 publications

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“…This is consistent with the results 166 of other assays that have found band-pass tuning for IPI in both sexes (Bennet-Clark and 167 Ewing, 1969;Rybak et al, 2002b;F. Von Schilcher, 1976;Zhou et al, 2015) and a sex-168 specific sign of locomotor responses (Crossley et al, 1995;F. Von Schilcher, 1976).…”

Section: Methods) 147 148supporting

confidence: 80%

“…In the 255 central brain there are ~70 Dsx+ neurons per hemisphere in females and ~140 Dsx+ 256 neurons per hemisphere in males (Kimura et al, 2015;Rideout et al, 2010). Previous 257 studies found calcium responses to both song-like stimuli and pheromones in Dsx+ neuron 258 projections in females (Zhou et al, 2014) and tuning for the inter-pulse interval in males 259 (Zhou et al, 2015). In addition, silencing subsets of Dsx+ neurons in females affected 260 receptivity (Zhou et al, 2014).…”

mentioning

confidence: 99%

“…1A) (Bennet-Clark and Ewing, 1969;Rybak et al, 2002b;F. Von 142 Schilcher, 1976;Zhou et al, 2015). Using FLyTRAP, we systematically compared male and 143 female locomotor tuning to 82 acoustic stimuli that span the features and timescales present 144 in courtship song (see Supplemental Table 1).…”

mentioning

confidence: 99%

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Shared Song Detector Neurons in Drosophila Male and Female Brains Drive Sex-Specific Behaviors

Deutsch

Clemens

Thiberge

et al. 2018

Preprint

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19Males and females often produce distinct responses to the same sensory stimuli. How such 20 differences arise -at the level of sensory processing or in the circuits that generate behavior 21 -remains largely unresolved across sensory modalities. We address this issue in the 22 acoustic communication system of Drosophila. During courtship, males generate time-23 varying songs, and each sex responds with specific behaviors. We characterize male and 24 female behavioral tuning for all aspects of song, and show that feature tuning is similar 25 between sexes, suggesting sex-shared song detectors drive divergent behaviors. We then 26 identify higher-order neurons in the Drosophila brain, called pC2, that are tuned for multiple 27 temporal aspects of one mode of the male's song, drive sex-specific behaviors, and show a 28 mirrored correspondence between sensory and motor tuning. We thus uncover acoustic 29 object detector neurons at the sensory-motor interface that flexibly link auditory perception 30 with sex-specific behavioral responses to communication signals.

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Section: Methods) 147 148supporting

confidence: 80%

mentioning

confidence: 99%

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Shared Song Detector Neurons in Drosophila Male and Female Brains Drive Sex-Specific Behaviors

Deutsch

Clemens

Thiberge

et al. 2018

Preprint

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“…The upstream neuron is then optogenetically activated while the downstream neuron expressing GCaMP6 is monitored for changes in fluorescence (Figure 6c). These experiments can be done with the nervous system kept in the body or using a dissected brain (Kallman et al 2015;Zhou et al 2015;Clowney et al 2015;Hampel et al 2015;Cohn et al 2015;Shirangi et al 2016). The functional connectivity between neurons that is demonstrated by measuring GECI responses cannot indicate whether the connections are direct, and it is always possible that intermediate neurons are involved.…”

Section: Assessing Functional Connectivity Among Neuronsmentioning

confidence: 99%

Targeted manipulation of neuronal activity in behaving adult flies

Hampel

Seeds

2016

Preprint

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The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource ABSTRACTThe ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.

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“…For example, the Drosophila JO contains about 500 mechanosensory cells, $75% of which (subgroups A, B and D) have been linked to auditory function [71], but the ascending mechanosensory pathways for conspecific song detection are likely to have substantially fewer neurons [71][72][73]. In insect auditory systems, individuali.e., unique -neurons can be identified, and in some species a single neuron alone can drive important behaviors, such as the bat-evasion reflex in noctuid moths [74].…”

Section: Curbio 13179mentioning

confidence: 99%

Comparative Aspects of Hearing in Vertebrates and Insects with Antennal Ears

Albert¹,

Kozlov

2016

Current Biology

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The evolution of hearing in terrestrial animals has resulted in remarkable adaptations enabling exquisitely sensitive sound detection by the ear and sophisticated sound analysis by the brain. In this review, we examine several such characteristics, using examples from insects and vertebrates. We focus on two strong and interdependent forces that have been shaping the auditory systems across taxa: the physical environment of auditory transducers on the small, subcellular scale, and the sensory-ecological environment within which hearing happens, on a larger, evolutionary scale. We briefly discuss acoustical feature selectivity and invariance in the central auditory system, highlighting a major difference between insects and vertebrates as well as a major similarity. Through such comparisons within a sensory ecological framework, we aim to emphasize general principles underlying acute sensitivity to airborne sounds.Introduction Auditory physiology offers a distinctive perspective on the interaction between a sensory system and its environment. On the one hand, auditory systems in vertebrates and insects with sensitive hearing are capable of remarkable performances on multiple levels, both within the sensory periphery where the minute energies associated with sound are converted into electrical signals, as well as within higher-order brain areas where complex natural stimuli such as human speech are processed. For example, displacements caused by acoustical stimuli in the inner ear at the threshold of hearing are sub-nanometer and comparable to the distance between atoms in molecules [1]. Furthermore, thermal fluctuations in the ear's mechanotransduction apparatus not only are significant, but also can be larger than the faintest audible signals, making signal detection a challenging task [2]. Ascending the sensory hierarchy, one encounters other marvels of evolution, such as the ability of individual neurons to encode -using millisecond-long action potentials -inter-aural time differences of only about ten microseconds, and to use this information to localize the source of the sound [3,4]. No engineered system has yet been designed that could understand distorted speech in a noisy and reverberating environment with multiple speakers. That our auditory system achieves this feat is testament to its remarkable performance.On the other hand, an engineer could argue that the auditory system's performance is objectively poor, even in animals with sensitive hearing: at the very first step, during the mechanoelectrical transduction in the inner ear, external sounds are distorted, or even completely suppressed, while new tones are generated by the ear itself [5]. Forward and backward masking, illusory percepts of nonexistent tones (such as the Zwicker illusion [6]), perceptual merging of separate auditory streams, the precedence effect suppressing the perception of echoes that has been demonstrated in insects and vertebrates [7,8] (and which some blind people can unsuppress), auditory hallucinations, and a frustrating inab...

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Central neural circuitry mediating courtship song perception in male Drosophila

Cited by 77 publications

References 67 publications

Shared Song Detector Neurons in Drosophila Male and Female Brains Drive Sex-Specific Behaviors

Shared Song Detector Neurons in Drosophila Male and Female Brains Drive Sex-Specific Behaviors

Targeted manipulation of neuronal activity in behaving adult flies

Comparative Aspects of Hearing in Vertebrates and Insects with Antennal Ears

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