Centralized isolation of<i>Helicobacter pylori</i>from multiple centers and transport condition influences

Gong, Yanan; Li, Youming; Li, Hongzhang; Guo, Feng; Lin, Lang; Wang, Qunying; Zhang, Jiakun; Ji, Zizhong; Mao, Jibo; Mao, Junliang; Shi, Zhengchao; Tang, Wuheng; Zhu, Xiao-liang; Shao, Wei; Zhang, Xiaofeng; Wang, Xinghua; Tong, Yue-Feng; Jiang, Mizu; Chen, Guang-lan; Wang, Zhiyong; Huimin, Tu; Jiang, Guofa; Wu, Jiansheng; Chen, Xu-Peng; Ding, Qiulong; Ouyang, Hong; Jin, Fengzhe; Zhang, Jianzhong

doi:10.3748/wjg.v21.i3.944

Cited by 14 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Though selective medium containing antibiotic additives (nalidixic acid, polymyxin B, and bacitracin) was used for H. pylori isolation, non-H. pylori still grew and affected the growth of H. pylori in some cases, and the positive rate of H. pylori isolation was only approximately 30%, which is similar to that reported previously. [52][53][54] To improve the positive rate of isolation, inoculation was performed immediately after sampling or transporting samples at low temperatures, using dry ice to keep them cold, and adding nalidixic and bacitracin along with H. pylori-selective supplements in the medium to avoid the growth of non-H. pylori. This is a small samplesize study, but we did find a general heterogeneity of H. pylori in the stomachs of local patients.…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Zhu

et al. 2021

IDR

View full text Add to dashboard Cite

Chronic Helicobacter pylori infection causes peptic ulcers in a subpopulation of individuals and is a risk factor for the development of gastric cancer. Multiple infections and heteroresistant H. pylori contribute to poor treatment efficacy. Here, we investigated the extent of genetic diversity among H. pylori strains within a given host and its influence on the results of antibiotic (metronidazole, levofloxacin, clarithromycin, amoxicillin, and tetracycline) susceptibility testing. Materials and Methods: Gastric mucosa biopsy samples were obtained from patients with gastric disorders, including 48 H. pylori positive patients, who were never previously treated for H. pylori infection. Five potential H. pylori colonies isolated from each sample were subcultured for enrichment. Enriched H. pylori colonies were identified through Gram staining and assays for urease, oxidase, and catalase. For each H. pylori monoclonal colony, the antibiotic susceptibility was assessed, genomic DNA was sequenced, and the cytotoxinassociated gene A (cagA) genotype was verified. Co-infection with multiple H. pylori strains was determined using random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR). Results: Thirteen gastric mucosa biopsy samples were positive for H. pylori. Five monoclonal strains isolated from each of these 13 patients were identified as H. pylori. RAPD-PCR indicated that intra-patient monoclonal strains of H. pylori in 10 of the 13 samples exhibited heterogeneity. Among the 13 patients, intra-patient monoclonal strains isolated from 4 patients had identical cagA genotype, whereas intra-patient monoclonal strains isolated from the other 9 patients harbored more than one cagA genotype. The antibiotic susceptibility of five intra-patient monoclonal strains from seven patients was inconsistent. Conclusion: The existence of heterogeneous H. pylori strains with resistance to different drugs and virulence were common within the gastric mucosa of an individual patient.

show abstract

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Zhu

et al. 2021

IDR

View full text Add to dashboard Cite

show abstract

“…Some investigators suggested that rapid transport of biopsy specimens in an appropriate transport medium at low temperature is essential for a satisfactory recovery of H pylori 3,12 . Others demonstrated that H pylori can survive at room temperature for 24 hours without loss of the viability, indicating that low temperature is not essential for the survival of H pylori 13,14 . Most importantly, rapid transport and specialized transport media had been considered important for high diagnostic yield 12,14 .…”

Section: Discussionmentioning

confidence: 99%

“…Others demonstrated that H pylori can survive at room temperature for 24 hours without loss of the viability, indicating that low temperature is not essential for the survival of H pylori 13,14 . Most importantly, rapid transport and specialized transport media had been considered important for high diagnostic yield 12,14 . Several transport media have been studied, including Stuart's media, urea‐containing saline, and normal saline 12,13,15‐17 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

et al. 2020

View full text Add to dashboard Cite

Background and Aims Isolation of Helicobacter pylori is considered difficult because of the requirement of the additional biopsy tissue and the effort involved in the isolation of the bacterium. We investigated whether H pylori can be cultured from tissue samples used for the rapid urease test (RUT). Methods Totally, 174 specimens from 87 patients referred for endoscopy were prospectively included. During endoscopy, two biopsy specimens were obtained, one each from the gastric antrum and the corpus, and were placed into a commercially available RUT kit. After detection of urease activity, H pylori was cultured using tissue leftover in the RUT, regardless of the result. Results H pylori was successfully isolated using leftover tissue in 72.4% (63/87) of the patients. In 32 patients, H pylori was isolated from both specimens, while in 31 patients, it was isolated from either antrum or corpus. Eighty‐one H pylori strains were isolated from 141 specimens with positive RUT results (57.4%), whereas 14 strains were isolated from 33 specimens with negative RUT results (42.4%). The median interval between tissue acquisition and inoculation onto the isolation media was 3.6 hours (range: 0.5‐27.5 hours) in cases with successful cultures, compared to 23.5 hours (range: 0.5‐76.0 hours) in cases with failed cultures. Among the positive RUT tissues, 80.4% (45/56) were cultured successfully when the tissue was inoculated within 4 hours of the biopsy. Conclusions RUT kits can be used as transport media for H pylori, and this media is most efficient when used within 4 hours of the test.

show abstract

“…The dental plaques were broken and cultivated on Columbia agar plates (Oxoid of Thermo Fisher Scientific Inc., Waltham, MA, United States). The rapid urease test (RUT), a commercially available rapid gastric urease test kit, was used to for detecting HP, and the detailed methods of culture and polymerase chain reaction (PCR) are described elsewhere [ 24 , 29 , 30 ]. The sample was identified as positive HP if the test gel color changed from yellow to red within 20 min, up to a maximum of 60 min [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Association Between Helicobacter pylori Infection and Risk of Periodontal Diseases in Han Chinese: A Case-Control Study

et al. 2016

View full text Add to dashboard Cite

BackgroundThis study was performed to test the association between Helicobacter pylori (HP) and periodontal disease (PD).Material/MethodsThis was a case-control study in a comprehensive hospital, including all patients with newly diagnosed PD between 2012 and 2014 as cases and all patients without PD as controls, thorough periodontal examinations. Those who tested positive for HP were examined by means of polymerase chain reaction. Single and multivariate logistic regression was used to analyze the data using SPSS 19.0 software.ResultsThis case-control study included 212 Han Chinese non-smoking adults. The results indicated that HP-positive status significantly increased the risk of PD (2.63 times higher (odds ratio [OR]=2.63; 95% confidence interval [CI]=1.48–4.67). After adjustment for age, sex, level of education, physical exercise, body mass index, and history of alcohol and diabetes mellitus, this association remained significantly (OR=2.82, 95% CI=1.55–5.13).ConclusionsPD might be associated with HP infection in adults and HP infection may be a significant and independent risk factor for PD.

show abstract

Centralized isolation ofHelicobacter pylorifrom multiple centers and transport condition influences

Abstract: Transportation of specimens within 24 h and below 24 °C is reasonable and acceptable for centralized culture of multicenter H. pylori samples.

Cited by 14 publications

References 27 publications

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

Association Between Helicobacter pylori Infection and Risk of Periodontal Diseases in Han Chinese: A Case-Control Study

Contact Info

Product

Resources

About