Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats

Kawasaki, Makoto; Onaka, Tatsushi; Nakazato, Masamitsu; Saito, Jun; Mera, Takashi; Hashimoto, Hirofumi; Fujihara, Hiroaki; Okimoto, Nobukazu; Ohnishi, Hideo; Nakamura, Toshio; Ueta, Yoichi

doi:10.1677/joe.1.06636

Cited by 9 publications

(4 citation statements)

References 37 publications

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“…Taylor et al (2005) demonstrated activation of the hypothalamicpituitary-adrenal axis because of NPW23 activity in the parvocellular PVN. Kawasaki et al (2006) reported that centrally administered NPW caused activation of magnocellular neurosecretory neurons in the SON and PVN as well as significant increases in plasma arginine-vasopressin and oxytocin levels. Administration of NPW is also believed to significantly increase Fos expression in PVN and SON cells (Niimi and Fig.…”

Section: Discussionmentioning

confidence: 99%

Cloning and distribution of neuropeptide W and its receptors in pigs

Fang

Zheng

et al. 2015

Research in Veterinary Science

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Section: Discussionmentioning

confidence: 99%

Cloning and distribution of neuropeptide W and its receptors in pigs

Fang

Zheng

et al. 2015

Research in Veterinary Science

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“…There is an apparently contradictory study showing that both OT and VP plasma levels can be elevated by i.c.v. NPW (21); however, this can be reconciled if, in the study showing elevated OT and VP, the OT and VP neurones had low activity levels, such that NPW‐induced depolarisation (and therefore release) would be the observed response to its introduction into the central nervous system.…”

Section: Discussionmentioning

confidence: 99%

Neuropeptide W has Cell Phenotype‐Specific Effects on the Excitability of Different Subpopulations of Paraventricular Nucleus Neurones

Price¹,

Samson²,

Ferguson³

2009

J Neuroendocrinology

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Administration of the neuropeptides NPW and NPB in rodents has been shown to influence the activity of a variety of autonomic and neuroendocrine systems. The paraventricular nucleus is a major autonomic and neuroendocrine integration site in the hypothalamus and neurons within this nucleus express the receptor for these ligands, neuropeptide B/W receptor 1 (NPBWR1). Therefore we used whole cell patch clamp recordings coupled with single cell RT-PCR to examine the effects of neuropeptide W-23 (NPW-23) on the excitability of identified paraventricular nucleus neurons. Oxytocin, vasopressin and thyrotropin releasing hormone neurons were all found to be responsive to 10 nM NPW-23, although both depolarizing and hyperpolarizing effects were observed in each of these cell groups. In contrast corticotropin releasing hormone cells were unaffected. Further subdivision of chemically phenotyped cell groups into magnocellular, neuroendocrine or pre-autonomic neurons, using their electrophysiological fingerprints, revealed that neurons projecting to medullary and spinal targets were predominantly inhibited by NPW-23 while those that projected to median eminence or neural lobe showed nearly equivalent numbers of depolarizing and hyperpolarizing cells. The demonstration of particular phenotypic populations of paraventricular nucleus neurons showing NPW-induced effects on excitability reinforces the importance of the NPB/NPW neuropeptide system as a regulator of autonomic function.

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“…Noxious stimuli for example, or intracerebroventricular injection of certain neuromodulators can influence transcription (Kawasaki et al, 2006;Kurose et al, 2001;Nomura et al, 1999;Onaka et al, 2003), and the specific mechanism(s) by which either physiological stimuli or exogenous substances result in magnocellular VP transcription remains unknown. With regards to the stimulus used in the current study, it has been shown by several independent laboratories that transcription of VP increases in response to a hyperosmotic stimulus (see Supp Table 1).…”

Section: Discussionmentioning

confidence: 99%

An intron-based real-time PCR method for measuring vasopressin gene transcription

Ponzio

Yue

Gainer

2007

Journal of Neuroscience Methods

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The hypothalamus contains distinct neuronal populations that express distinguishing neuropeptides. The supraoptic nucleus contains magnocellular neurons that predominantly express either vasopressin or oxytocin. Transcriptional activators of vasopressin and other neuropeptides have been the subject of much research. Here we present a method of measuring neuropeptide transcription by tailoring one-step quantitative real-time PCR (qRT-PCR) for the analysis of processed and premRNA (heteronuclear RNA). Using moderate and strong hyperosmotic stimuli to induce transcription, we report an increase in vasopressin transcription (pre-mRNA) of 141% and 406% over control levels in response to a 2% injection of 900 mOsm saline or a 1% body weight i.p. injection of 2M NaCl, respectively. These results agree with a host of studies employing the more laborintensive method of in situ hybridization histochemistry by which investigators also measured intron-containing heteronuclear RNAs. Furthermore, these results confirm that qRT-PCR with intron-specific primers can be used to rapidly analyze transcription, and suggest an important further benefit of a real-time PCR analysis, such as the ability of measuring transcription of multiple neuropeptides along with other genes from a single sample.

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Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats

Cited by 9 publications

References 37 publications

Cloning and distribution of neuropeptide W and its receptors in pigs

Cloning and distribution of neuropeptide W and its receptors in pigs

Neuropeptide W has Cell Phenotype‐Specific Effects on the Excitability of Different Subpopulations of Paraventricular Nucleus Neurones

An intron-based real-time PCR method for measuring vasopressin gene transcription

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