Centrifugal partition chromatography in the isolation of minor ecdysteroids from Cyanotis arachnoidea

Issaadi, Halima Meriem; Tsai, Yi‐Hung; Chang, Fang-Rong; Hunyadi, Attila

doi:10.1016/j.jchromb.2017.03.043

Cited by 17 publications

(21 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on our results, identical spectra of detected ecdysteroids in N. noctula and P. nathusii was in accordance with their common most preferred food items (Figure 1), but not with their significantly different body weight. Assuming that the spectrum of blood-borne ecdysteroids is interrelated with the taxonomic diversity of insect food items, these results confirm previous observations, according to which dietary diversity was not related to the body mass of bats 6 . At the same time, variations in the spectra of ecdysteroids according to sampling months might reflect changes in the daily or seasonal availability/uptake of insects from various taxonomic orders.…”

supporting

confidence: 90%

Ecdysteroids as natural doping substances in the blood of insectivorous bats

Hornok

Berkecz

E³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Bats have deserved much scientific attention due to their biological-ecological properties and increasingly recognized epidemiological significance. Ecdysteroids are insect-molting hormones that (under experimental conditions) have stimulating and anabolic effects in mammals, including humans. Therefore, these biologically active compounds are currently under consideration by the World Anti-Doping Agency to become doping-controlled substances. Previously we demonstrated that low to high concentrations of ecdysteroids appear in the blood of insectivorous passerine birds. Since passerine birds and echolocating bats share several adaptive mechanisms in connection with flying, and insectivorism is also among their common traits, we hypothesized that ecdysteroids might also be present in the blood of insectivorous bats. To test this hypothesis, blood samples of eight insectivorous bat species were collected and analyzed for the presence of ecdysteroids with highly sensitive targeted ultra-high-performance liquid chromatography coupled to high-resolution quadrupole-orbitrap mass spectrometry method (UHPLC-HRMS). The results supported our hypothesis, because nine ecdysteroids were detected in bat blood. The spectrum of these ecdysteroids was similar in those bat species which have their most preferred food items from the same insect order, supporting insects as the most likely source of these hormones. It was also shown that the spectrum of blood-borne ecdysteroids was broader in the autumn than in the summer, and higher concentrations of 20-hydroxyecdysone were measured in samples of large size bat species in comparison with small size ones. Based on the known physiologic effects of ecdysteroids, we postulate that these results might have implications on the metabolic rate and parasite burdens of insectivorous bats.

show abstract

supporting

confidence: 90%

Ecdysteroids as natural doping substances in the blood of insectivorous bats

Hornok

Berkecz

E³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Standard ecdysteroids 20-hydroxyecdysone (20E; 1 ), 20E 2- and 3-acetate ( 2 and 3 , respectively), 20E 20,22-acetonide ( 4 ), 2-deoxy-20E ( 5 ), ajugasterone C ( 6 ), calonysterone ( 7 ), dacryhainansterone ( 8 ), ecdysone ( 9 ), 9,11-didehydropoststerone ( 10 ), 2-deoxypoststerone ( 11 ), polypodine B ( 12 ), poststerone ( 13 ), rubrosterone ( 14 ), shidasterone ( 15 ), 20E 22-acetate ( 16 ), 5α-20E ( 17 ), 20E 2,3;20,22-diacetonide ( 18 ), 5β-hydroxypoststerone ( 19 ), 11α-hydroxypoststerone ( 20 ), the 17β-acyl analog of calonysterone ( 21 ), ajugalactone ( 22 ), cyasterone ( 23 ), herkesterone ( 24 ), and 3- epi -20E ( 25 ) were obtained from our previous phytochemical studies 29–32 . Compounds 1 and 4 – 25 possessed a purity of >95%.…”

Section: Methodsmentioning

confidence: 99%

Ecdysteroids are present in the blood of wild passerine birds

Hornok

Csorba

Kováts³

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Ecdysteroids (arthropod molting hormones) play an important role in the development and sexual maturation of arthropods, and they have been shown to have anabolic and “energizing” effect in higher vertebrates. The aim of this study was to assess ecdysteroid diversity, levels according to bird species and months, as well as to observe the molting status of hard ticks (Acari: Ixodidae) infesting the birds. Therefore, blood samples and ticks were collected from 245 birds (244 songbirds and a quail). Mass spectrometric analyses showed that 15 ecdysteroids were regularly present in the blood samples. Molting hormones biologically most active in insects (including 20-hydroxyecdysone [20E], 2deoxy-20E, ajugasterone C and dacryhainansterone) reached different levels of concentration according to bird species and season. Similarly to ecdysteroids, the seasonal presence of affected, apolytic ticks peaked in July and August. In conclusion, this study demonstrates the presence of a broad range and high concentrations of ecdysteroids in the blood stream of wild-living passerine birds. These biologically active, anabolic compounds might possibly contribute to the known high metabolic rate of songbirds.

show abstract

“…Moreover, the high selection basis of plants and solvents, development of advanced extraction, separation and purification techniques, and/or analytical methods allow the selection and design of customized fractions from initial concentrate. A special case is Centrifugal Partition Chromatography (Figure 10), which avoids the use of solid phase separation columns (which are expensive and prone to degrade sensitive compounds) with a rotor of special construction that allows the simultaneous utilization of a liquid stationary phase and a liquid mobile phase [105,[135][136][137]. Table 1 presents various applications of plant extracts for a wide range of foods.…”

Section: Plant Extractsmentioning

confidence: 99%

Edible and Functionalized Films/Coatings—Performances and Perspectives

et al. 2020

View full text Add to dashboard Cite

In recent years, food packaging has evolved from an inert and polluting waste that remains after using the product toward an active item that can be consumed along with the food it contains. Edible films and coatings represent a healthy alternative to classic food packaging. Therefore, a significant number of studies have focused on the development of biodegradable enveloping materials based on biopolymers. Animal and vegetal proteins, starch, and chitosan from different sources have been used to prepare adequate packaging for perishable food. Moreover, these edible layers have the ability to carry different active substances such as essential oils—plant extracts containing polyphenols—which bring them considerable antioxidant and antimicrobial activity. This review presents the latest updates on the use of edible films/coatings with different compositions with a focus on natural compounds from plants, and it also includes an assessment of their mechanical and physicochemical features. The plant compounds are essential in many cases for considerable improvement of the organoleptic qualities of embedded food, since they protect the food from different aggressive pathogens. Moreover, some of these useful compounds can be extracted from waste such as pomace, peels etc., which contributes to the sustainable development of this industry.

show abstract

Centrifugal partition chromatography in the isolation of minor ecdysteroids from Cyanotis arachnoidea

Cited by 17 publications

References 23 publications

Ecdysteroids as natural doping substances in the blood of insectivorous bats

Ecdysteroids as natural doping substances in the blood of insectivorous bats

Ecdysteroids are present in the blood of wild passerine birds

Edible and Functionalized Films/Coatings—Performances and Perspectives

Contact Info

Product

Resources

About