Centrifugal planar‐column chromatography (CPCC): A new preparative planar technique. Part 1: Description of the method and practical aspects

Nyiredy, Sz.; Mészáros, Szilvia; Nyiredy-Mikita, K.; Dallenbach-Toelke, K.; Sticher, Otto

doi:10.1002/jhrc.1240091017

Cited by 13 publications

(10 citation statements)

References 5 publications

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“…supercritical fluid extraction, microwave-assisted extraction, pressurized-fluid extraction, forced-flow solidliquid extraction; and, automated extraction methods [17].…”

Section: Extractionmentioning

confidence: 99%

“…Rotation planar extraction (RPE) is a forced-flow solidliquid extraction (FFSLE) technique wherein the extraction solvent(s) migrate(s) mainly through centrifugal force [17,21]. A novel prototype instrument for multi-functional separation, ExtraChrom ® , enables the rotation planar extraction of complex matrices, because a planar column can be attached to it and then filled with the material to be extracted.…”

Section: Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

282

187

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Natural derived or originated compounds still play a major role as drugs, and as lead structures for the development of synthetic molecules. About 50% of the drugs introduced to the market during the last 20 years are derived directly or indirectly from small biogenic molecules. In the future, natural products will continue to play a major role as active substances, model molecules for the discovery and validation of drug targets. A multidisciplinary approach to drug discovery involving the generation of truly novel molecular diversity from natural product sources, combined with total and combinatorial synthetic methodologies provides the best solution to increase the productivity in drug discovery and development. Screening for new drugs in plants implies the screening of extracts for the presence of novel compounds and an investigation of their biological activities. It is currently estimated that approximately 420,000 plant species exist in nature. For the purpose of lead discovery, or for the scientific validation of a traditional medicinal plant or a phytopharmaceutical, active principals in complex matrices need to be identified. Therefore, the interfacing of biological and chemical assessment becomes the critical issue. Drug discovery from plants can be guided by epidemiologic studies facilitated with computer assisted HPLC microfractionation and microplate technology. Epidemiologic studies have shown that high dietary flavonoid intake may be associated with decreased risk for cardiovascular disease. Chlamydia pneumoniae is a common human pathogen and epidemiological and clinical studies have shown a connection between chronic C. pneumoniae infection, atherosclerosis and the risk of myocardial infarction. We will present here the detection of natural compounds active against C. pneumoniae as an example.

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“…supercritical fluid extraction, microwave-assisted extraction, pressurized-fluid extraction, forced-flow solidliquid extraction; and, automated extraction methods [17].…”

Section: Extractionmentioning

confidence: 99%

Section: Extractionmentioning

confidence: 99%

Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

282

187

View full text Add to dashboard Cite

show abstract

“…The extraction column was filled with 110 g sample of plant material and was subjected to exhaustive MPSLE extraction with ethyl acetate for a combined dissolution and diffusion time [11] of 6 h. Fractions were monitored by TLC; the total area of the densitograms between 0.1 and 0.8 was determined at s = 313 nm. After the extraction process had been finished, two samples were taken: one from the top and another from the bottom of the extraction column.…”

Section: Samples Of Extractionmentioning

confidence: 99%

“…with rotation planar extraction [11] solid-liquid extractions can be carried out in a closed circular chamber with a special geometric design in which all profiles of the planar column have a constant surface along the radius in order to ensure linear solvent flow. Although the extraction distance is relatively short compared with other conventional preparative extraction techniques, the separation efficiency is higher due to the fine particle sizes of the solid phase to be extracted and variable centrifugal force.…”

Section: Introductionmentioning

confidence: 99%

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

Nyiredy¹,

Botz

2003

Chromatographia

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SummaryA novel variant of continuous relative counter-current extraction, called medium-pressure solid-liquid extraction (MPSLE) is introduced as a new preparative separation method for laboratory purposes. This method is a special case of exhaustive extraction.On using MPSLE, the extraction column, i.e. a medium pressure liquid chromatographic (MPLC) column is filled with the fine-powdered solid phase to be extracted, and extraction solvent(s) is/are forced through the stationary bed by means of a pump. This method constitutes the relative counter-current extraction, and results in exhaustive and rapid extraction. By MPSLE, efficiency is achieved not just because the extraction solvent(s) is/are forced through the staying material to be extracted, but also clue to the overpressure in the extraction column and the suitable changes of the dissolution and diffusion periods of the process.The method can be used for the rapid extraction of various substance classes occurring as complex solid matrices. Experiments carried out with MPLC columns show that the exhaustive extraction of finely powdered plant material of 100-3000g can be extracted and concentrated with an automated equipment within a few hours. Further advantages of the environment-friendly process performed in closed system are full automation and operation with low qua ntil',/of extracta nt.

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“…[4,5] A novel multi-functional separation instrument, prototype ExtraChrom 1 , even enables the rotation planar extraction of complex matrices, because a planar column can easily be attached to it and then filled with the material to be extracted. The factors affecting the RPE process are basically the same as in MPSLE, with the exception that the solvent is driven by centrifugal force instead of a pump and the geometry of the column differs from that of the MPLC columns used in MPSLE.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

Yrjönen

Vovk

Simonovska

et al. 2003

Journal of Liquid Chromatography & Related Technologies

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The effect of three operating variables on the extraction efficiency of medium pressure solid-liquid extraction (MPSLE) was studied using a 3289 factorial experimental design with three levels. The operating variables tested were medium particle size of the plant material, volume of extractant, and the equilibrium time between consecutive extractions of the same material. The possibility of scaling up the MPSLE extraction process on larger columns was also confirmed. The extraction efficiency did not markedly decrease, and a systematic increase in the extraction yields was observed on scaling up the MPSLE procedure. The results of the MPSLE experiments were compared with those obtained by the rotation planar extraction (RPE) method using a prototype ExtraChrom 1 separation instrument with particle size of the plant material and volume of extractant as the operating variables. Both methods proved to be suitable for the efficient extraction of the leaves of various Ficus species. The strong points of RPE were the ease of operation of the ExtraChrom 1 separation instrument and significant time savings achieved, whereas the possibility of scaling up the extraction process made MPSLE a suitable method for even larger scale preparative extractions.

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Centrifugal planar‐column chromatography (CPCC): A new preparative planar technique. Part 1: Description of the method and practical aspects

Cited by 13 publications

References 5 publications

Natural Products in the Process of Finding New Drug Candidates

Natural Products in the Process of Finding New Drug Candidates

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

Comparison of Medium Pressure Solid–Liquid Extraction and Rotation Planar Extraction of Ficus Leaves with Reference to Optimum Operating Parameters

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