Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

Abstract

Waterborne pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. We present an innovative centrifugal sedimentation immunoassay platform for detection of bacterial pathogens in water. Our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a densitymedia in a microfluidic disk. Beads at the distal end of the disk are imaged to quantify the fluorescence and determine the bacterial concentration. Our platform is fast (20 min), can detect as few as $10 bacteria with minimal sample preparation, and can detect multiple pathogens simultaneously. The platform was used to detect a panel of enteric bacteria (Escherichia coli, Salmonella typhimurium, Shigella, Listeria, and Campylobacter) spiked in tap and ground water samples. V C 2016 AIP Publishing LLC. [http://dx

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

“…We present here a portable centrifugal microfluidic system that uses a sedimentation-based immunoassay, reported in previous work by our group [30,31,32,33], to detect a panel of four pathogenic bacteria: E. coli , Listeria , Salmonella , and Shigella . The immunoassays were performed on simple, inexpensive microfluidic discs that were loaded into a compact, battery-powered instrument to perform centrifugation and subsequent detection using laser-induced fluorescence (LIF).…”

Listeria monocytogenes meningoencephalomyelitis most likely due to septic spread as a rare cause of neurological disease and fever in an adult horse

“…Especially in ruminants, but also in rare equine cases, infection is often associated with feeding of (improperly fermented) silage 3 10 16 . Listeria species have also been detected in groundwater and water tanks, which therefore represent another important source of infection 17 18. To the authors’ knowledge, this is the second report describing histopathological changes in an adult horse with encephalitis due to listeriosis and the first report describing an involvement of the spinal cord.…”

New strategies for the enumeration of enteric pathogens in water

“…This method was used on spiked samples of tap and groundwater, but pre‐filtration through Whatman paper was necessary to remove large particulates. The method could detect pathogen as low as 10 CFU/assay (Litvinov et al ., ).…”