Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells

Dawe, Helen R.; Farr, Helen; Gull, Keith

doi:10.1242/jcs.03305

Cited by 233 publications

(213 citation statements)

References 128 publications

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“…The apical localization of centrioles is thought to be one of the prerequisites for primary ciliogenesis [23,24]. As dynein plays a key role in the movement of centrosomes in many cellular events, such as cell division and cell differentiation [5], loss of Dlic1 might impair ciliogenesis by affecting the centriole localization.…”

Section: Loss Of Dlic1 Does Not Affect Apical Location Of Centrioles mentioning

confidence: 99%

Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein

Kong

Peng

et al. 2013

Cell Res

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Cytoplasmic dynein 1 is fundamentally important for transporting a variety of essential cargoes along microtubules within eukaryotic cells. However, in mammals, few mutants are available for studying the effects of defects in dynein-controlled processes in the context of the whole organism. Here, we deleted mouse Dlic1 gene encoding DLIC1, a subunit of the dynein complex. Dlic1 −/− mice are viable, but display severe photoreceptor degeneration. Ablation of Dlic1 results in ectopic accumulation of outer segment (OS) proteins, and impairs OS growth and ciliogenesis of photoreceptors by interfering with Rab11-vesicle trafficking and blocking efficient OS protein transport from Golgi to the basal body. Our studies show that Dlic1 deficiency partially blocks vesicle export from endoplasmic reticulum (ER), but seems not to affect vesicle transport from the ER to Golgi. Further mechanistic study reveals that lack of Dlic1 destabilizes dynein subunits and alters the normal subcellular distribution of dynein in photoreceptors, probably due to the impaired transport function of dynein. Our results demonstrate that Dlic1 plays important roles in ciliogenesis and protein transport to the OS, and is required for photoreceptor development and survival. The Dlic1 −/− mice also provide a new mouse model to study human retinal degeneration.

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Section: Loss Of Dlic1 Does Not Affect Apical Location Of Centrioles mentioning

confidence: 99%

Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein

Kong

Peng

et al. 2013

Cell Res

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“…Cytoplasmic microtubules are organized from an appendage called the basal foot that decorates the mature centriole, which is also seen in multiciliated cells in other animal lineages [97,98]. In addition, the mature centriole anchors a prominent striated ciliary rootlet that connects the base of the flagellum to the accessory centriole and to the nucleus [97].…”

Section: (Iii) Early Metazoamentioning

confidence: 99%

Exploring the evolutionary history of centrosomes

Azimzadeh

2014

Phil. Trans. R. Soc. B

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The centrosome is the main organizer of the microtubule cytoskeleton in animals, higher fungi and several other eukaryotic lineages. Centrosomes are usually located at the centre of cell in tight association with the nuclear envelope and duplicate at each cell cycle. Despite a great structural diversity between the different types of centrosomes, they are functionally equivalent and share at least some of their molecular components. In this paper, we explore the evolutionary origin of the different centrosomes, in an attempt to understand whether they are derived from an ancestral centrosome or evolved independently from the motile apparatus of distinct flagellated ancestors. We then discuss the evolution of centrosome structure and function within the animal lineage.

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“…They are required for the organization of interphase and mitotic microtubule arrays and as basal bodies they serve as templates for the growth of nine doublet microtubules in axonemes of cilia and flagella (for review see Marshall, 2007). Although flowering plants and more derived fungi have reduced or eliminated centrioles and cilia, metazoans have diversified their functions in numerous sensory and developmental processes (for reviews see Singla and Reiter, 2006;Dawe et al, 2007). Several mammalian genetic disorders have been linked to defects in centrioles or cilia (for review see Afzelius, 2004;Pan et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Centrioles in actively dividing cells show conservative replication and semiconservative segregation during division (for review see Dawe et al, 2007). As a result, each pair of centrioles consists of an older and a younger centriole that differ in age by at least one cell generation.…”

Section: Introductionmentioning

confidence: 99%

“…Several mammalian genetic disorders have been linked to defects in centrioles or cilia (for review see Afzelius, 2004;Pan et al, 2005). For example, the proteins associated with Bardet-Biedl syndrome, a debilitating pleiotropic human disorder characterized by obesity, renal abnormalities, polydactyly, retinal degeneration, and genitourinary tract malformations have been shown to localize to or around centrioles and cilia (for review see Beales, 2005;Blacque and Leroux, 2006).Centrioles in actively dividing cells show conservative replication and semiconservative segregation during division (for review see Dawe et al, 2007). As a result, each pair of centrioles consists of an older and a younger centriole that differ in age by at least one cell generation.…”

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confidence: 99%

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The Uni2 Phosphoprotein is a Cell Cycle–regulated Component of the Basal Body Maturation Pathway inChlamydomonas reinhardtii

Piasecki

LaVoie

Tam

et al. 2008

MBoC

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Mutations in the UNI2 locus in Chlamydomonas reinhardtii result in a "uniflagellar" phenotype in which flagellar assembly occurs preferentially from the older basal body and ultrastructural defects reside in the transition zones. The UNI2 gene encodes a protein of 134 kDa that shares 20.5% homology with a human protein. Immunofluorescence microscopy localized the protein on both basal bodies and probasal bodies. The protein is present as at least two molecular-weight variants that can be converted to a single form with phosphatase treatment. Synthesis of Uni2 protein is induced during cell division cycles; accumulation of the phosphorylated form coincides with assembly of transition zones and flagella at the end of the division cycle. Using the Uni2 protein as a cell cycle marker of basal bodies, we observed migration of basal bodies before flagellar resorption in some cells, indicating that flagellar resorption is not required for mitotic progression. We observed the sequential assembly of new probasal bodies beginning at prophase. The uni2 mutants may be defective in the pathways leading to flagellar assembly and to basal body maturation. INTRODUCTIONCentrioles are evolutionarily ancient organelles, possibly extant in a common ancestor of all eukaryotic cells (CavalierSmith, 2002). They are required for the organization of interphase and mitotic microtubule arrays and as basal bodies they serve as templates for the growth of nine doublet microtubules in axonemes of cilia and flagella (for review see Marshall, 2007). Although flowering plants and more derived fungi have reduced or eliminated centrioles and cilia, metazoans have diversified their functions in numerous sensory and developmental processes (for reviews see Singla and Reiter, 2006;Dawe et al., 2007). Several mammalian genetic disorders have been linked to defects in centrioles or cilia (for review see Afzelius, 2004;Pan et al., 2005). For example, the proteins associated with Bardet-Biedl syndrome, a debilitating pleiotropic human disorder characterized by obesity, renal abnormalities, polydactyly, retinal degeneration, and genitourinary tract malformations have been shown to localize to or around centrioles and cilia (for review see Beales, 2005;Blacque and Leroux, 2006).Centrioles in actively dividing cells show conservative replication and semiconservative segregation during division (for review see Dawe et al., 2007). As a result, each pair of centrioles consists of an older and a younger centriole that differ in age by at least one cell generation. The "mother" centriole differs both in structure and function from the "daughter" centriole. For example, in the single pair of centrioles found in most types of mammalian cells, the mother centriole nucleates the growth of the primary cilium and contains proteins not present in the daughter centriole. The daughter centriole becomes competent for ciliary assembly only in the ensuing cell cycle (for review see Bornens, 2002). Extension of the pathway for sequential maturation of basal bodies has been ...

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Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells

Cited by 233 publications

References 128 publications

Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein

Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein

Exploring the evolutionary history of centrosomes

The Uni2 Phosphoprotein is a Cell Cycle–regulated Component of the Basal Body Maturation Pathway inChlamydomonas reinhardtii

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