Centroacinar cells: At the center of pancreas regeneration

Beer, Rebecca; Parsons, Michael; Rovira, Meritxell

doi:10.1016/j.ydbio.2016.02.027

Cited by 54 publications

(53 citation statements)

References 104 publications

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“…However, little is known about the presence of some salivary gland cells that could show a progenitor function. Interestingly, it has been reported that the CACs of the pancreas in adult mouse and zebrafish showed a progenitor function and may contribute to the maintenance of tissue homeostasis [31,32]. Similarly, we revealed the presence of centroacinar-like cells in the goat submandibular salivary glands and we hypothesize that cells could have a regenerative role.…”

Section: Discussionsupporting

confidence: 70%

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Elewa¹,

Elsayed²,

Mohamed³

et al. 2017

J Vet Sci Technol

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The centroacinar cells (CACs) are a specialized ductal pancreatic cell. Recently, it has been revealed that such cells showed a progenitor function in the pancreas of adult mouse and zebrafish. However, no information about its occurrence in other exocrine glands specially in farm animals. Cytokeratins immunohistochemistry was used to detect ductal segments of the submandibular glands and the pancreas including CACs in human and rat. In the present study, the detection of cytokeratins in the normal goat pancreas (a serous type gland) and mandibular salivary gland (a mixed type gland, consisted of mucous tubules capped with serous demilunes) was investigated using a standard immunoperoxidase technique on paraffin sections. In the goat pancreas and mandibular salivary gland, cytokeratins were found in all duct cells including the CACs of the pancreas. Interestingly in the mandibular salivary glands, some immunoreacted cells with immunopositive cytoplasmic extensions were frequently observed between the cytoplasm of the mucous cells, especially near the lumina of the mucous tubules. We postulate that these cells could be centroacinar-like cells. Therefore, from these findings, it can be concluded that these cells could provide a supportive cytoskeleton to maintain cellular shape and avoid the collapse of the mucous cells during mucous secretion. Additionally, a regenerative function for such cells could be hypothesized. Furthermore, the keratin demonstration could be used as a marker for ductal cells and is a useful aid in the detection of lesions derived from gland ductal cells in goat. Further research is necessary to investigate the expression of keratins in caprine glandular tumors as well as the regenerative ability of these centroacinar-like cells in goat mandibular salivary glands injury.

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Section: Discussionsupporting

confidence: 70%

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Elewa¹,

Elsayed²,

Mohamed³

et al. 2017

J Vet Sci Technol

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“…Repair and regeneration of the injured organ are orchestrated by many cell types including acinar cells, centroacinar cells, ductal cells, immune cells, and stellate cells 7, 8. The existence of a resident stem cell population in the organ remains controversial 9, 10.…”

mentioning

confidence: 99%

Acinar-to-Ductal Metaplasia Induced by Transforming Growth Factor Beta Facilitates KRAS G12D -driven Pancreatic Tumorigenesis

Chuvin

Vincent

Pommier

et al. 2017

Cellular and Molecular Gastroenterology and Hepatology

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Background & AimsTransforming growth factor beta (TGFβ) acts either as a tumor suppressor or as an oncogene, depending on the cellular context and time of activation. TGFβ activates the canonical SMAD pathway through its interaction with the serine/threonine kinase type I and II heterotetrameric receptors. Previous studies investigating TGFβ-mediated signaling in the pancreas relied either on loss-of-function approaches or on ligand overexpression, and its effects on acinar cells have so far remained elusive.MethodsWe developed a transgenic mouse model allowing tamoxifen-inducible and Cre-mediated conditional activation of a constitutively active type I TGFβ receptor (TβRICA) in the pancreatic acinar compartment.ResultsWe observed that TβRICA expression induced acinar-to-ductal metaplasia (ADM) reprogramming, eventually facilitating the onset of KRASG12D-induced pre-cancerous pancreatic intraepithelial neoplasia. This phenotype was characterized by the cellular activation of apoptosis and dedifferentiation, two hallmarks of ADM, whereas at the molecular level, we evidenced a modulation in the expression of transcription factors such as Hnf1β, Sox9, and Hes1.ConclusionsWe demonstrate that TGFβ pathway activation plays a crucial role in pancreatic tumor initiation through its capacity to induce ADM, providing a favorable environment for KRASG12D-dependent carcinogenesis. Such findings are highly relevant for the development of early detection markers and of potentially novel treatments for pancreatic cancer patients.

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“…The majority of the exocrine tissue constitutes the acinar part of the gland (Houbracken & Bouwens, ), which is organized into pancreatic lobules, that is, acini (Geron, Schejter, & Shilo, ; Low, Shukla, & Thorn, ). The function of the acini is synthesis, storage, and secretion of digestive enzymes, for example, α‐amylases, lipases and proteases, and acinar fluid (Beer, Parsons, & Rovira, ; Leung & Ip, ). However, enzyme and fluid secretion are separate processes because secretion of fluid is dependent on movement of ions (chloride and sodium) and is related to osmotic flow of water from the cell into the duct.…”

Section: Introductionmentioning

confidence: 99%

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2019

Journal of Morphology

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This study report about the differentiation of pancreatic acinar tissue in grass snake, Natrix natrix, embryos using light microscopy, transmission electron microscopy, and immuno‐gold labeling. Differentiation of acinar cells in the embryonic pancreas of the grass snake is similar to that of other amniotes. Pancreatic acini occurred for the first time at Stage VIII, which is the midpoint of embryonic development. Two pattern of acinar cell differentiation were observed. The first involved formation of zymogen granules followed by cell migration from ducts. In the second, one zymogen granule was formed at the end of acinar cell differentiation. During embryonic development in the pancreatic acini of N. natrix, five types of zymogen granules were established, which correlated with the degree of their maturation and condensation. Within differentiating acini of the studied species, three types of cells were present: acinar, centroacinar, and endocrine cells. The origin of acinar cells as well as centroacinar cells in the pancreas of the studied species was the pancreatic ducts, which is similar as in other vertebrates. In the differentiating pancreatic acini of N. natrix, intermediate cells were not present. It may be related to the lack of transdifferentiation activity of acinar cells in the studied species. Amylase activity of exocrine pancreas was detected only at the end of embryonic development, which may be related to animal feeding after hatching from external sources that are rich in carbohydrates and presence of digestive enzymes in the egg yolk. Mitotic division of acinar cells was the main mechanism of expansion of acinar tissue during pancreas differentiation in the grass snake embryos.

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Centroacinar cells: At the center of pancreas regeneration

Cited by 54 publications

References 104 publications

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Acinar-to-Ductal Metaplasia Induced by Transforming Growth Factor Beta Facilitates KRAS G12D -driven Pancreatic Tumorigenesis

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

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