Centromere-Linkage Analysis and Consolidation of the Zebrafish Genetic Map

Johnson, Stephen L.; Gates, Michael A.; Johnson, Michele Nicole; Talbot, William S.; Horne, Sally; Baik, Kris; Rude, Sunny; Wong, Jamie R.; Postlethwait, John H.

doi:10.1093/genetics/142.4.1277

Cited by 164 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effort was greatly aided by the sequencing of the zebrafish genome, beginning in 2001 at the Sanger Institute and completed in 2013 (Howe et al., ). The genome of zebrafish contains approximately 26,000 genes over 1.4 billion base pairs on 25 pairs of chromosomes (Daga, Thode, & Amores, ; Howe et al., ; Johnson et al., ). Thus, the zebrafish genome contains approximately the same number of protein coding genes as the human genome in about half the size, and over a similar number of chromosomes).…”

Section: Use Of Zebrafish As a Laboratory Organismmentioning

confidence: 99%

Zebrafish: Development of a Vertebrate Model Organism

Meyers

2018

CP Essential Lab Tech

145

View full text Add to dashboard Cite

Zebrafish (Danio rerio) are a small-bodied tropical, freshwater fish species originally from South Asia. The ease of care, year-round prolific breeding, and transparent, external development have made these fish a popular model vertebrate for many fields of biology. Over the past 40 years, the development of methods for genetic and developmental manipulation have driven a rapid rise in their use in research. This review provides an orientation to the zebrafish as a model system, from its ecology and life history to its early use in research and adoption as a key genetic model for vertebrate development. Its current uses and key techniques that highlight future areas of development of the system are also discussed. Finally, an introduction to the essentials of zebrafish use and care are presented, highlighting the ease of adoption of this system for teaching and research use. C 2018 by John Wiley & Sons, Inc.Keywords: zebrafish r Danio rerio r development r model organism r genetics r regeneration r fish

show abstract

Section: Use Of Zebrafish As a Laboratory Organismmentioning

confidence: 99%

Zebrafish: Development of a Vertebrate Model Organism

Meyers

2018

CP Essential Lab Tech

145

View full text Add to dashboard Cite

show abstract

Zebrafishstat3 is expressed in restricted tissues during embryogenesis andstat1 rescues cytokine signaling in aSTAT1-deficient human cell line

et al. 1999

View full text Add to dashboard Cite

Transcription factors of the STAT family are required for cellular responses to multiple signaling molecules. After ligand binding‐induced activation of cognate receptors, STAT proteins are phosphorylated, hetero‐ or homodimerize, and translocate to the nucleus. Subsequent STAT binding to specific DNA elements in the promoters of signal‐responsive genes alters the transcriptional activity of these loci. STAT function has been implicated in the transduction of signals for growth, reproduction, viral defense, and immune regulation. We have isolated and characterized two STAT homologs from the zebrafish Danio rerio. The stat3 gene is expressed in a tissue‐restricted manner during embryogenesis, and larval development with highest levels of transcript are detected in the anterior hypoblast, eyes, cranial sensory ganglia, gut, pharyngeal arches, cranial motor nuclei, and lateral line system. In contrast, the stat1 gene is not expressed during early development. The stat3 gene maps to a chromosomal position syntenic with the mouse and human STAT3 homologs, whereas the stat1 gene does not. Despite a higher rate of evolutionary change in stat1 relative to stat3, the stat1 protein rescues interferon‐signaling functions in a STAT1‐deficient human cell line, indicating that cytokine‐signaling mechanisms are likely to be conserved between fish and tetrapods. Dev Dyn 1999;215:352–370. © 1999 Wiley‐Liss, Inc.

show abstract