Centromere proteins and chromosome inheritance: a complex affair

Dobie, Kenneth W; Hari, Kumar; Maggert, Keith A.; Karpen, Gary H.

doi:10.1016/s0959-437x(99)80031-8

Cited by 84 publications

(39 citation statements)

References 117 publications

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“…The functional role of the centromere in cell division, including both meiosis and mitosis, is highly conserved in all eukaryotic species. Several proteins involved in centromeric function have been found to be conserved in highly divergent eukaryotic species, including S. cerevisiae, Caenorhabditis elegans, D. melanogaster, human, and plants (for reviews, see Dobie et al, 1999;. However, the centromeric DNA sequences are extremely variable even among closely related species.…”

Section: Discussionmentioning

confidence: 99%

Functional Rice Centromeres Are Marked by a Satellite Repeat and a Centromere-Specific Retrotransposon

et al. 2002

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The centromere of eukaryotic chromosomes is essential for the faithful segregation and inheritance of genetic information. In the majority of eukaryotic species, centromeres are associated with highly repetitive DNA, and as a consequence, the boundary for a functional centromere is difficult to define. In this study, we demonstrate that the centers of rice centromeres are occupied by a 155-bp satellite repeat, CentO, and a centromere-specific retrotransposon, CRR . The CentO satellite is located within the chromosomal regions to which the spindle fibers attach. CentO is quantitatively variable among the 12 rice centromeres, ranging from 65 kb to 2 Mb, and is interrupted irregularly by CRR elements. The break points of 14 rice centromere misdivision events were mapped to the middle of the CentO arrays, suggesting that the CentO satellite is located within the functional domain of rice centromeres. Our results demonstrate that the CentO satellite may be a key DNA element for rice centromere function.

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Section: Discussionmentioning

confidence: 99%

Functional Rice Centromeres Are Marked by a Satellite Repeat and a Centromere-Specific Retrotransposon

et al. 2002

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“…Heterochromatin formation in the centromeric region is critical for sister chromatid synapsis, spindle formation, and chromosome segregation (Dobie et al 1999;Sullivan et al 2001;Sharp et al 2003). As DDP1 was found to associate with pericentromeric heterochromatin in Drosophila (Cortes et al 1999;Cortes and Azorin 2000), we asked whether vigilin KH13-14 might disrupt the heterochromatin status in the pericentromeric region.…”

Section: Expression Of Vigilin Kh13-14 Destabilizes Heterochromatinmentioning

confidence: 99%

On the mechanism of induction of heterochromatin by the RNA-binding protein vigilin

Zhou

Carmichael²

2008

RNA

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Vigilin is an RNA-binding protein localized to both the cytoplasm and the nucleus and has been previously implicated in heterochromatin formation and chromosome segregation. We demonstrate here that the C-terminal domain of human vigilin binds to the histone methyltransferase SUV39H1 in vivo. This association is independent of RNA and maps to a site on vigilin that is not involved in its interaction with several other known protein partners. Cells that express high levels of the C-terminal fragment display chromosome segregation defects, and ChIP analyses show changes in the status of pericentric b-satellite and rDNA chromatin from heterochromatic to more euchromatic form. Finally, a cell line with inducible expression of the vigilin Cterminal fragment displays inducible alterations in b-satellite chromatin. These and other results lead us to present a new model for vigilin-mediated, RNA-induced gene silencing.

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“…Whereas there are no universally conserved DNA sequences associated with centromere function, several proteins specific to the centromere/kinetochore complex are highly conserved (Dobie et al, 1999;Yu et al, 2000;Houben and Schubert, 2003). A centromere-specific histone H3-like protein, referred to as CENH3, has been found to underlie the kinetochore (see reviews in Henikoff et al, 2001;Sullivan et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Maize Centromeres: Organization and Functional Adaptation in the Genetic Background of Oat

et al. 2004

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Centromeric DNA sequences in multicellular eukaryotes are often highly repetitive and are not unique to a specific centromere or to centromeres at all. Thus, it is a major challenge to study the fine structure of individual plant centromeres. We used a DNA fiber-fluorescence in situ hybridization approach to study individual maize (Zea mays) centromeres using oat (Avena sativa)-maize chromosome addition lines. The maize centromere-specific satellite repeat CentC in the addition lines allowed us to delineate the size and organization of centromeric DNA of individual maize chromosomes. We demonstrate that the cores of maize centromeres contain mainly CentC arrays and clusters of a centromere-specific retrotransposon, CRM. CentC and CRM sequences are highly intermingled. The amount of CentC/CRM sequence varies from ;300 to >2800 kb among different centromeres. The association of CentC and CRM with centromeric histone H3 (CENH3) was visualized by a sequential detection procedure on stretched centromeres. The analysis revealed that CENH3 is always associated with CentC and CRM but that not all CentC or CRM sequences are associated with CENH3. We further demonstrate that in the chromosomal addition lines in which two CenH3 genes were present, one from oat and one from maize, the oat CENH3 was consistently incorporated by the maize centromeres.

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Centromere proteins and chromosome inheritance: a complex affair

Cited by 84 publications

References 117 publications

Functional Rice Centromeres Are Marked by a Satellite Repeat and a Centromere-Specific Retrotransposon

Functional Rice Centromeres Are Marked by a Satellite Repeat and a Centromere-Specific Retrotransposon

On the mechanism of induction of heterochromatin by the RNA-binding protein vigilin

Maize Centromeres: Organization and Functional Adaptation in the Genetic Background of Oat

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