Centromeres Reposition to the Nuclear Periphery during L6E9 Myogenesisin Vitro

Chaly, Nathalie; Munro, Sandra B.

doi:10.1006/excr.1996.0082

Cited by 58 publications

(36 citation statements)

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“…Reversion of malignant HMECs in 3D culture is accompanied by the establishment of features of higher order nuclear structure Concentration of heterochromatin at the nucleolar and/or nuclear periphery and formation of enlarged splicing factor speckles are nuclear features of differentiation in several cell types, including mammary epithelial cells (Manuelidis, 1985;Antoniou et al, 1993;Chaly and Munro, 1996;Lelièvre et al, 1998;Martou and De Boni, 2000;Gribbon et al, 2002;Beil et al, 2002;Garagna et al, 2004;Abad et al, 2007). To assess whether features of nuclear organization that are characteristic of mammary acinar differentiation are lost in breast cancer cells, we cultured non-neoplastic S1 cells (Briand et al, 1987) and S1-derived malignant T4-2 cells (Briand et al, 1996) in Matrigel TM in 3D for 10 days.…”

Section: Resultsmentioning

confidence: 99%

The control of tissue architecture over nuclear organization is crucial for epithelial cell fate

Chandramouly

Abad

Knowles

et al. 2007

Journal of Cell Science

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The remodeling of nuclear organization during differentiation and the dramatic alteration of nuclear organization associated with cancer development are well documented. However, the importance of tissue architecture in the control of nuclear organization remains to be determined. Differentiation of mammary epithelial cells into functional tissue structures, in three-dimensional culture, is characterized by a specific tissue architecture (i.e. a basoapical polarity axis), cell cycle exit and maintenance of cell survival. Here we show that induction of partial differentiation (i.e. basal polarity only, cell cycle exit and cell survival) by epigenetic mechanisms in malignant breast cells is sufficient to restore features of differentiation-specific nuclear organization, including perinucleolar heterochromatin, large splicing factor speckles, and distinct nuclear mitotic apparatus protein (NuMA) foci. Upon alteration of nuclear organization using an antibody against NuMA, differentiated non-neoplastic cells undergo apoptosis, whereas partially differentiated malignant cells enter the cell cycle. Non-neoplastic cells cultured under conditions that prevent the establishment of apical polarity also enter the cell cycle upon NuMA antibody treatment. These findings demonstrate that the differentiation status rather than the non-neoplastic or neoplastic origin of cells controls nuclear organization and suggest a link between nuclear organization and epigenetic mechanisms dictated by tissue architecture for the control of cell behavior.

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Section: Resultsmentioning

confidence: 99%

The control of tissue architecture over nuclear organization is crucial for epithelial cell fate

Chandramouly

Abad

Knowles

et al. 2007

Journal of Cell Science

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“…Peripheral repositioning of centromeres is the most common structural change associated with differentiation in human and mouse cell types (Chaly and Munro, 1996;Bá rtová et al, 2001;Harničarová et al, 2006). Wiblin et al (2005) reported that the proportion of centromeres located close to the nuclear periphery in hESCs is smaller than that in highly differentiated B-cells.…”

Section: Discussionmentioning

confidence: 99%

Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation

Bártová

Galiová

Krejčí

et al. 2008

Developmental Dynamics

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“…Several studies have already dealt with the description of centromere localization and distribution in mammalian cells during interphase (18,22,36,37). One of the few studies on centromere distributions in 3D was published by Martou and De Boni (21).…”

Section: Discussionmentioning

confidence: 99%

“…During postnatal development of mice, dynamic clustering processes of centromeres associated with various stages of differentiation were demonstrated in Purkinje cells (21). A centromere repositioning to the nuclear periphery was observed during L6E9 myoblast differentiation (22). Although several studies already characterized various aspects of centromere localization and clustering, no quantitative approach has yet been used to evaluate global patterns of the spatial centromere distribution.…”

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confidence: 99%

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

et al. 2002

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Background:The pericentromeric heterochromatin is an important element for the regulation of gene silencing. Its spatial distribution during interphase appears to be celltype specific. This study analyzes three-dimensional (3D) centromere distribution patterns during cellular differentiation along the neutrophil pathway. Methods: Differentiation of the promyelocytic leukemia cell line NB4 was induced by retinoic acid. Centromeres in interphase nuclei were visualized by immunofluorescence staining of centromere-associated proteins with CREST serum. 3D images of nuclei were obtained by confocal microscopy. Automated methods for the segmentation of point-like objects in 3D images were implemented to detect the position of centromeres. Features of centromere localization patterns were determined by constructing the minimal spanning tree of the centromere distribution.

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Centromeres Reposition to the Nuclear Periphery during L6E9 Myogenesisin Vitro

Cited by 58 publications

References 0 publications

The control of tissue architecture over nuclear organization is crucial for epithelial cell fate

The control of tissue architecture over nuclear organization is crucial for epithelial cell fate

Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

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