CEPHALOPHARYNGEAL SKELETONS OF Chrysomya megacephala (Fabricius, 1794) (DIPTERA: CALLIPHORIDAE) THIRD INSTAR LARVAE DISPLAYED RESISTANCE TO HOT-WATER KILLING METHOD – IMPLICATIONS IN FORENSIC ENTOMOLOGY PRACTICE

ABDULLAH, SHARIFAH SHAKILAH; Zuha, Raja Muhd

doi:10.55230/mabjournal.v49i5.1644

Cited by 2 publications

(1 citation statement)

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“…Since forensic entomology practice mainly deals with specimens stored in preservatives, such as in 70-95% ethanol (Amendt et al, 2007), it is imperative to observe variations of size caused by the effect of preservatives on the larval body and how it differs from cephalopharyngeal skeleton. Furthermore, the rigid sclerites of the cephalopharyngeal skeleton could potentially provide a better estimation of PMI min than highly flexible larval body length which was prone to physical distortions due to killing procedure and preservation effects (Tantawi & Greenberg, 1993;Adams & Hall, 2003;Rosilawati et al, 2014;Abdullah & Zuha, 2020;Zuha, 2021). Even so, the prospect of using cephalopharyngeal skeleton as an alternative growth indicator to larval body length relies on the sample representation across different ages and instars, including differing outcomes when using other species than C. megacephala as experimental subjects.…”

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confidence: 99%

A PRELIMINARY EVALUATION ON BIVARIATE ALLOMETRY IN ACTIVE-FEEDING Chrysomya megacephala (FABRICIUS, 1794) (DIPTERA: CALLIPHORIDAE) LARVAE

Zuha

2022

MABJ

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The potential use of larval cephalopharyngeal skeleton as an alternative growth indicator in forensic entomology practice was assessed based on its developmental pattern, growth performance and allometric relationship with larval body. Chrysomya megacephala (Fabricius, 1794), was used as an experimental species and larval development were studied at ambient temperatures and relative humidity. Larval body size was measured from furthest part of the head to the last abdominal segment. Cephalopharyngeal skeleton was extracted from the body and measured from the tip of dorsal bridge to the left face of dorsal cornu. Daily progression of larval body length and cephalopharyngeal skeleton length showed the latter significantly had slower growth rates. The allometry of larval body and cephalopharyngeal skeleton showed they were correlated only because both variables increased across the three larval instars, which suggesting a spurious correlation. Separate bivariate correlations between the two variables showed only the first instar larvae had a significant (p<0.01) but weak correlation, r=0.33. However, removing the effect of larval instars still produced statistically significant correlation (p<0.001) albeit with moderate strength, r=0.57. From this study, there was not enough evidence to support cephalopharyngeal skeleton as an equal or a better alternative to larval body as growth indicator.

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