Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures

Lopes, Vanessa M.; Sampaio, Eduardo; Roumbedakis, Katina; Tanaka, Nobuaki; Carulla, Lucía; Gambús, Guillermo; Woo, Theodosia; Martins, Catarina P. P.; Pénicaud, Virginie; Gibbings, Colette; Eberle, Jessica S.; Tedesco, Perla; Fernández, Irene Mirón; Rodríguez-González, Tania; Imperadore, Pamela; Ponte, Giovanna; Fiorito, Graziano

doi:10.1007/s10158-017-0200-4

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…All work was performed in compliance with the EU Directive 2010/63/EU on cephalopod use and AAALAC guidelines on the care and welfare of cephalopods 69 – 71 . We sequenced the genome of D. pealeii using a whole genome shotgun approach that combined long single-molecule PacBio reads with short, high accuracy paired-end Illumina data (Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Genome and transcriptome mechanisms driving cephalopod evolution

et al. 2022

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Cephalopods are known for their large nervous systems, complex behaviors and morphological innovations. To investigate the genomic underpinnings of these features, we assembled the chromosomes of the Boston market squid, Doryteuthis (Loligo) pealeii, and the California two-spot octopus, Octopus bimaculoides, and compared them with those of the Hawaiian bobtail squid, Euprymna scolopes. The genomes of the soft-bodied (coleoid) cephalopods are highly rearranged relative to other extant molluscs, indicating an intense, early burst of genome restructuring. The coleoid genomes feature multi-megabase, tandem arrays of genes associated with brain development and cephalopod-specific innovations. We find that a known coleoid hallmark, extensive A-to-I mRNA editing, displays two fundamentally distinct patterns: one exclusive to the nervous system and concentrated in genic sequences, the other widespread and directed toward repetitive elements. We conclude that coleoid novelty is mediated in part by substantial genome reorganization, gene family expansion, and tissue-dependent mRNA editing.

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Section: Methodsmentioning

confidence: 99%

Genome and transcriptome mechanisms driving cephalopod evolution

et al. 2022

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“…These cephalopod experiments were performed in compliance with the EU Directive 2010/63/EU guidelines on cephalopod use, the University of Chicago Animal Resources Center and the MBL and UChicago Institutional Animal Care and Use Committees 44,45 .…”

Section: Methodsmentioning

confidence: 99%

Neuronal segmentation in cephalopod arms

Olson,

Schulz,

Ragsdale

2024

Preprint

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The prehensile arms of the cephalopod are among these animals most remarkable features, but the neural circuitry governing arm and sucker movements remains largely unknown. We studied the neuronal organization of the adult axial nerve cord (ANC) ofOctopus bimaculoideswith molecular and cellular methods. The ANCs, which lie in the center of every arm, are the largest neuronal structures in the octopus, containing four times as many neurons as found in the central brain. In transverse cross section, the cell body layer (CBL) of the ANC wraps around its neuropil (NP) with little apparent segregation of sensory and motor neurons or nerve exits. Strikingly, when studied in longitudinal sections, the ANC is segmented. ANC neuronal cell bodies form columns separated by septa, with 15 segments overlying each pair of suckers. The segments underlie a modular organization to the ANC neuropil: neuronal cell bodies within each segment send the bulk of their processes directly into the adjoining neuropil, with some reaching the contralateral side. In addition, some nerve processes branch upon entering the NP, forming short-range projections to neighboring segments and mid-range projections to the ANC segments of adjoining suckers. The septa between the segments are employed as ANC nerve exits and as channels for ANC vasculature. Cellular analysis establishes that adjoining septa issue nerves with distinct fiber trajectories, which across two segments (or three septa) fully innervate the arm musculature. Sucker nerves also use the septa, setting up a nerve fiber “suckerotopy” in the sucker-side of the ANC. Comparative anatomy suggests a strong link between segmentation and flexible sucker-laden arms. In the squidDoryteuthis pealeii, the arms and the sucker- rich club of the tentacles have segments, but the sucker-poor stalk of the tentacles does not. The neural modules described here provide a new template for understanding the motor control of octopus soft tissues. In addition, this finding represents the first demonstration of nervous system segmentation in a mollusc.

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“…This animal study was reviewed and approved by the Institutional Animal Care and Use Committee at the Marine Biological Laboratory, Woods Hole, MA, USA protocol number 22-13F, in compliance with the EU Directive 2010/63/EU on cephalopod use and AAALAC guidelines on the care and welfare of cephalopods (Fiorito et al, 2014(Fiorito et al, , 2015Lopes et al, 2017).…”

Section: Animal Husbandrymentioning

confidence: 99%

Vessel sound causes hearing loss for hummingbird bobtail squid (Euprymna berryi)

Putland

Mooney²,

Mensinger³

2023

Front. Mar. Sci.

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Anthropogenic activity and its associated sounds have been shown to incur adverse effects on the behaviour and physiology of a wide range of aquatic taxa, from marine mammals to fishes. Yet, little is known about how invertebrates detect and respond to anthropogenic sound. The hummingbird bobtail squid (Euprymna berryi) has a short lifespan (< 6 months), grows to sexual maturity around 90 days post hatching and its small size (< 5 cm mantle length) makes the species an ideal candidate to examine potential effects of sound exposure under laboratory conditions. Hearing and behavioural observations were made before, during and after 15 minutes of vessel sound playback, and aural sensitivity curves were determined using auditory evoked potentials. A significant decrease in relative ventilation rate was observed during and post sound exposure. Auditory sensitivity before and after vessel sound exposure was also examined for three different ages: juveniles, mid- and late adults. Baseline audiograms indicated that there was a decrease in aural sensitivity with age. All three age groups showed similar, significantly decreased hearing sensitivity following sound exposure, however auditory sensitivity recovered within two hours. Globally, anthropogenic sounds have become louder and more persistent, therefore there may be limited time for these animals to recover from sound exposure. Given their ecological and economic importance, cephalopods should be considered in management and policy on underwater noise owing to potential adverse effects of anthropogenic sound on behaviour and physiology.

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Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures

Cited by 11 publications

References 36 publications

Genome and transcriptome mechanisms driving cephalopod evolution

Genome and transcriptome mechanisms driving cephalopod evolution

Neuronal segmentation in cephalopod arms

Vessel sound causes hearing loss for hummingbird bobtail squid (Euprymna berryi)

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