Cephalosporin P1 and helvolic acid

Retinol and retinoic acid modulate the metabolism of 25‐hydroxyvitamin D3 in kidney cell culture

“…Results are expressed as the percentage of the radioactivity recovered from the HPLC. DNA was measured as in [18]. Synthetic 25(OH)D3 was kindly donated by Hoffmann-La Roche (Basle).…”

Hepatocyte targeting of adenine‐9‐β‐D‐arabinofuranoside 5′‐monophosphate (ara‐AMP) coupled to lactosaminated albumin

“…DNA was extracted according to [24], the radioactivity was counted and the concentration measured as in [25]. Administration of EHNA was necessary [3] because ara-A is >lOO-times less active in rodents than in primates, due to its more rapid metabolism [26].…”

Immunological assay of the mitochondrial acetyl‐CoA acetyltransferase in crude liver homogenate

“…Liver homogenates were prepared by conventional homogenization and centrifugations and the mitochondria were solubilized in a medium containing Abbreviation: mAAT, mitochondrial acetylCoA acetyltransferase ElsevierjNorth-Holland Biomedical Press 0.05 M sodium phosphate (pH 6.6), 1 mM EDTA, 0.5 mM dithioerythritol, 20% (w/v) glycerine and 0.5% (w/v) Triton X-100. Then the homogenates were centrifuged for 60 min at 105 000 X g. DNA was determined as in [8]. Acetyl-CoA acetyltransferase activity was assayed in the presence of 80 mM potassium [2].…”