Cephalothrix gen. nov. (Cyanobacteria): towards an intraspecific phylogenetic evaluation by multilocus analyses

Malone, Camila Francieli da Silva; Rigonato, Janaína; Laughinghouse, Haywood Dail; Schmidt, Éder C.; Bouzon, Zenilda L.; Wilmotte, Annick; Fiore, Marli Fátima; Sant’Anna, Célia Leite

doi:10.1099/ijs.0.000369

Cited by 29 publications

(16 citation statements)

References 50 publications

(63 reference statements)

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“…2012, da Silva Malone et al. 2015, McGregor and Sendall 2015, McGregor and Sendall 2019). Trichomes composed of short cells (4.8–70.0 μm wide, 1.7–5.0 μm long) contain aerotopes and irregularly arranged thylakoids.…”

Section: Resultsmentioning

confidence: 96%

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Strunecký

Ivanova

Mareš

2023

Journal of Phycology

123

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Cyanobacterial taxonomy is facing a period of rapid changes thanks to the ease of 16S rRNA gene sequencing and established workflows for description of new taxa. Since the last comprehensive review of the cyanobacterial system in 2014 until 2021, at least 273 species in 140 genera were newly described. These taxa were mainly placed into previously defined orders and families although several new families were proposed. However, the classification of most taxa still relied on hierarchical relationships inherited from the classical morphological taxonomy. Similarly, the obviously polyphyletic orders such as Synechococcales and Oscillatoriales were left unchanged. In this study, the rising number of genomic sequences of cyanobacteria and well‐described reference strains allowed us to reconstruct a robust phylogenomic tree for taxonomic purposes. A less robust but better sampled 16S rRNA gene phylogeny was mapped to the phylogenomic backbone. Based on both these phylogenies, a polyphasic classification throughout the whole phylum of Cyanobacteria was created, with ten new orders and fifteen new families. The proposed system of cyanobacterial orders and families relied on a phylogenomic tree but still employed phenotypic apomorphies where possible to make it useful for professionals in the field. It was, however, confirmed that morphological convergence of phylogenetically distant taxa was a frequent phenomenon in cyanobacteria. Moreover, the limited phylogenetic informativeness of the 16S rRNA gene, resulting in ambiguous phylogenies above the genus level, emphasized the integration of genomic data as a prerequisite for the conclusive taxonomic placement of a vast number of cyanobacterial genera in the future.

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Section: Resultsmentioning

confidence: 96%

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

Strunecký

Ivanova

Mareš

2023

Journal of Phycology

123

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“…Also, while Argonema is a predominantly soil cyanobacterium from areas with generally low precipitation, Cephalothrix genus is found in a tropical or sub-tropical freshwater environment. The type species, Cephalothrix komarekiana is a tropical freshwater species isolated from Brazilian Pantanal Wetlands 39 . Aerosakkonema funiforme is also a freshwater cyanobacterium isolated from the mesotrophic reservoir in the Lao People's Democratic Republic 40 .…”

Section: Resultsmentioning

confidence: 99%

New cyanobacterial genus Argonema is hiding in soil crusts around the world

Skoupý

Stanojković

Pavlikova

et al. 2022

Sci Rep

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Cyanobacteria are crucial primary producers in soil and soil crusts. However, their biodiversity in these habitats remains poorly understood, especially in the tropical and polar regions. We employed whole genome sequencing, morphology, and ecology to describe a novel cyanobacterial genus Argonema isolated from Antarctica. Extreme environments are renowned for their relatively high number of endemic species, but whether cyanobacteria are endemic or not is open to much current debate. To determine if a cyanobacterial lineage is endemic is a time consuming, elaborate, and expensive global sampling effort. Thus, we propose an approach that will help to overcome the limits of the sampling effort and better understand the global distribution of cyanobacterial clades. We employed a Sequencing Read Archive, which provides a rich source of data from thousands of environmental samples. We developed a framework for a characterization of the global distribution of any microbial species using Sequencing Read Archive. Using this approach, we found that Argonema is actually cosmopolitan in arid regions. It provides further evidence that endemic microbial taxa are likely to be much rarer than expected.

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“…in 2015 proposed for reclassification as Cephalothrix (da Silva Malone et al. 2015), and it is closely related to Phormidium strains that do not accumulate PHA based on staining, phaC PCR screening, and in some instances, 1 H NMR spectroscopy (Fig. 3).…”

Section: Discussionmentioning

confidence: 99%

Screening and characterization of polyhydroxyalkanoate granules, and phylogenetic analysis of polyhydroxyalkanoate synthase gene PhaC in cyanobacteria

Hong

Beld

Davis

et al. 2021

Journal of Phycology

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Using Nile Red and BODIPY 493/503 dye‐staining and fluorescence microscopy, twenty cyanobacterial strains, including ten commercially available strains and ten environmental isolates from estuaries, freshwater ponds, and lagoons, were screened for the accumulation of ecologically important and potentially biotechnologically significant carbon storage granules such as polyhydroxyalkanoates (PHA). Dye‐staining granules were observed in six strains. Three Synechocystis, spp. strains WHSYN, LSNM, and CGF‐1, and a Phormidium‐like sp. CGFILA were isolated from environmental sources and found to produce granules of polyhydroxyalkanoate (PHA) according to PHA synthase gene (phaC) PCR screening and 1H NMR analyses. The environmental isolate, Nodularia sp. Las Olas and commercially available Phormidium cf. iriguum CCALA 759 displayed granules but screened negative for PHA according to phaC PCR and 1H NMR analyses. Partial polyhydroxyalkanoate synthase subunit C (phaC) and 16S rRNA gene sequences obtained from the PHA‐accumulating strains and analyzed alongside publicly available phaC, phaE, 16S rRNA, and 23S rRNA data help in understanding the distribution and evolutionary history of PHA biosynthesis within the phylum Cyanobacteria. The data show that the presence of phaC is highly conserved within the genus Synechocystis, and present in at least one isolate of Phormidium. Maximum likelihood analyses and cophylogenetic modeling of PHA synthase gene sequences provide evidence of a recent horizontal gene transfer event between distant genera of cyanobacteria related to Pleurocapsa sp. PCC 7327 and Phormidium‐like sp. CGFILA. These findings will help guide additional screening for PHA producers, and may explain why some Phormidium species produce PHAs, while others do not.

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Cephalothrix gen. nov. (Cyanobacteria): towards an intraspecific phylogenetic evaluation by multilocus analyses

Cited by 29 publications

References 50 publications

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis

New cyanobacterial genus Argonema is hiding in soil crusts around the world

Screening and characterization of polyhydroxyalkanoate granules, and phylogenetic analysis of polyhydroxyalkanoate synthase gene PhaC in cyanobacteria

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