Cepharanthine Improves Renal Ischemia-Reperfusion Injury in Rats

Kusaka, Junya; Hagiwara, Satoshi; Hasegawa, Akira; Kudo, Kazuhiro; Koga, Hironori; Noguchi, Takayuki

doi:10.1016/j.jss.2010.01.025

Cited by 15 publications

(8 citation statements)

References 20 publications

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“…It was used for the treatment of various diseases such as the inhibition of free radicals, radiation-induced leukopenia, venomous snakebites, etc. [87,88]. At 30 µg/mL, cepharanthine had the ability of 94.6% inhibition on the peroxidation of linoleic acid [89].…”

Section: Antioxidant Activitiesmentioning

confidence: 96%

HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study

et al. 2020

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In this study, we investigate the phytochemical profile, anticancer, and antioxidant activities of Teucrium polium methanolic extract using both in vitro and in silico approaches. The results showed the identification of 29 phytochemical compounds belonging to 13 classes of compounds and 20 tripeptides using High Resolution-Liquid Chromatography Mass Spectrometry (HR-LCMS). 13R-hydroxy-9E,11Z octadecadienoic acid, dihydrosamidin, valtratum, and cepharantine were the main compounds identified. The tested extract showed promising antioxidant activities (ABTS-IC50 = 0.042 mg/mL; 1,1-diphenyl-2-picrylhydrazyl (DPPH)-IC50 = 0.087 mg/mL, β-carotene-IC50 = 0.101 mg/mL and FRAP-IC50 = 0.292 mg/mL). Using both malignant Walker 256/B and MatLyLu cell lines, T. polium methanolic extract showed a dose/time-dependent antitumor activity. The molecular docking approach revealed that most of the identified molecules were specifically binding with human peroxiredoxin 5, human androgen, and human progesterone receptors with high binding affinity scores. The obtained results confirmed that T. polium is a rich source of bioactive molecules with antioxidant and antitumor potential.

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Section: Antioxidant Activitiesmentioning

confidence: 96%

HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study

et al. 2020

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“…Intraperitoneal injection of vehicle or cepharanthine was performed immediately before reperfusion or at comparable time point in the sham groups. The dose of cepharanthine was determined according to previous reports [ 15 , 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies have demonstrated the beneficial effects of cepharanthine on several experimental models of inflammation [ 15 – 18 ]. Moreover, a recent study revealed that cepharanthine could attenuate acute kidney injury in a rat model of local renal I/R [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Salutary Effects of Cepharanthine against Skeletal Muscle and Kidney Injuries following Limb Ischemia/Reperfusion

Kao

Chung

Chang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Limb ischemia/reperfusion (I/R) causes oxidation and inflammation and subsequently induces muscle and kidney injuries. Cepharanthine, a natural plant alkaloid, possesses anti-inflammatory and antioxidative properties. We elucidated the salutary effects of cepharanthine against muscle and kidney injuries following limb I/R. Adult male rats were randomized to receive I/R or I/R plus cepharanthine. I/R was achieved by applying tourniquet high around each thigh for 3 hours followed by reperfusion for 24 hours. Cepharanthine (10 mg/kg, intraperitoneal) was injected immediately before reperfusion. After euthanization, degrees of tissue injury, inflammation, and oxidation were examined. Our data revealed that the I/R group had significant increases in injury biomarker concentrations of muscle (creatine kinase and lactate dehydrogenase) and kidney (creatinine, neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1). Histological assays revealed moderate muscle and kidney injury characteristics in the I/R group. The I/R group also had significant increases in concentrations of inflammatory molecules (interleukin-6, macrophage inflammatory protein-2, and prostaglandin E2) and reactive nitrogen species (nitric oxide) as well as lipid peroxidation (malondialdehyde). Of note, these effects of limb I/R could be mitigated by cepharanthine. These data confirmed that cepharanthine attenuated muscle and kidney injuries induced by limb I/R. The mechanisms may involve its anti-inflammatory and antioxidative capacities.

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“…CPA has been widely used for the treatment of many acute and chronic diseases, such as venomous snakebite, alopecia areata, exudative otitis media, and endotoxic shock (Furusawa & Wu 2007 ; Kusaka et al. 2011 ; Rogosnitzky & Danks 2011 ). CPA also exhibits potent anticancer activity in vitro for different cancer cells (Biswas et al.…”

Section: Introductionmentioning

confidence: 99%

Determination of cepharanthine in rat plasma by LC–MS/MS and its application to a pharmacokinetic study

Deng

et al. 2017

Pharmaceutical Biology

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Context: Cepharanthine (CPA) has been reported to possess a wide range of pharmacological activities.Objective: This study investigates the pharmacokinetic characteristics after oral or intravenous administration of CPA by using a sensitive and rapid LC–MS/MS method.Materials and methods: A sensitive and rapid LC–MS/MS method was developed for the determination of CPA in Sprague–Dawley rat plasma. Twelve rats were equally randomized into two groups, including the intravenous group (1 mg/kg) and the oral group (10 mg/kg). Blood samples (250 μL) were collected at designated time points and determined using this method. The pharmacokinetic parameters were calculated.Results: The calibration curve was linear within the range of 0.1–200 ng/mL (r = 0.999) with the lower limit of quantification at 0.1 ng/mL. After 1 mg/kg intravenous injection, the concentration of CPA reached a maximum of 153.17 ± 16.18 ng/mL and the t1/2 was 6.76 ± 1.21 h. After oral administration of 10 mg/kg of CPA, CPA was not readily absorbed and reached Cmax 46.89 ± 5.25 ng/mL at approximately 2.67 h. The t1/2 was 11.02 ± 1.32 h. The absolute bioavailability of CPA by oral route was 5.65 ± 0.35%, and the bioavailability was poor.Discussion and conclusions: The results indicate that the bioavailability of CPA was poor in rats, and further research should be conducted to investigate the reason for its poor bioavailability and address this problem.

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Cepharanthine Improves Renal Ischemia-Reperfusion Injury in Rats

Cited by 15 publications

References 20 publications

HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study

HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study

Salutary Effects of Cepharanthine against Skeletal Muscle and Kidney Injuries following Limb Ischemia/Reperfusion

Determination of cepharanthine in rat plasma by LC–MS/MS and its application to a pharmacokinetic study

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