Ceramide mediates tumor necrosis factor effects on P450-aromatase activity in cultured granulosa cells.

Santana, Pino; Llanes, L; Hernández, Inmaculada; Gallardo, Germán; Quintana, José; González, Javier Sangrós; Estévez, Francisco; Galarreta, Carlota Ruíz de; Fanjul, Luisa F.

doi:10.1210/endo.136.5.7720683

Cited by 56 publications

(28 citation statements)

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“…These observations support the hypothesis of an involvement of PLD in the stimulation of aldosterone secretion by S1P. Nonetheless, although ceramide blocks PLD, this may not be the only reason for the inhibition of aldosterone secretion by this sphingolipid, as ceramides can also affect the activities or the expression of enzymes that participate in steroid synthesis (52,65).…”

Section: S1p-stimulated Aldosterone Secretion Is Mediated By Pldsupporting

confidence: 82%

Sphingosine 1-phosphate: a novel stimulator of aldosterone secretion

Brizuela

Rábano

Peña

et al. 2006

Journal of Lipid Research

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Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid capable of regulating critical physiological and pathological functions. Here, we report for the first time that S1P stimulates aldosterone secretion in cells of the zona glomerulosa of the adrenal gland. Regulation of aldosterone secretion is important because this hormone controls electrolyte and fluid balance and is implicated in cardiovascular homeostasis. S1P-stimulated aldosterone secretion was dependent upon the protein kinase C (PKC) isoforms a and d and extracellular Ca 21 , and it was inhibited by pertussis toxin (PTX). S1P activated phospholipase D (PLD) through a PTX-sensitive mechanism, also involving PKC a and d and extracellular Ca 21 . Primary alcohols, which attenuate the formation of phosphatidic acid (the product of PLD), and cellpermeable ceramides, which inhibit PLD activity, blocked S1P-stimulated aldosterone secretion. Furthermore, propranolol, chlorpromazine, and sphingosine, which are potent inhibitors of phosphatidate phosphohydrolase (PAP) (the enzyme that produces diacylglycerol from phosphatidate), also blocked aldosterone secretion. These data suggest that the PLD/PAP pathway plays a crucial role in the regulation of aldosterone secretion by S1P and that Gi protein-coupled receptors, extracellular Ca 21 , and the PKC isoforms a and d are all important components in the cascade of events controlling this process.-Brizuela, L., M. Rábano, A. Peña, P. Gangoiti, J. M. Macarulla, M. Trueba, and A. Gómez-Muñoz. Sphingosine 1-phosphate: a novel stimulator of aldosterone secretion.

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Section: S1p-stimulated Aldosterone Secretion Is Mediated By Pldsupporting

confidence: 82%

Sphingosine 1-phosphate: a novel stimulator of aldosterone secretion

Brizuela

Rábano

Peña

et al. 2006

Journal of Lipid Research

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“…In this context, studies have shown that radiation may activate PKCa via diacylglycerol (DAG) generation (Chmura et al, 1996(Chmura et al, , 1997aBruno et al, 1998). As mentioned above, activation of PKC by phorbol esters blocked in most cell types tested radiation-induced ceramide generation and apoptosis (Haimovitz-Friedman et al, 1994a, b;Santana et al, 1995;Chmura et al, 1996;Allan-Yorke et al, 1998;Bruno et al, 1998). Conversely, pharmacologic inhibition of PKC enhanced radiation-induced cell death (Hallahan et al, 1992b).…”

Section: X-irradiationmentioning

confidence: 97%

“…Conversely, pharmacologic inhibition of PKC enhanced radiation-induced cell death (Hallahan et al, 1992b). The mechanism by which TPA and DAG inhibit apoptosis apparently via inhibition of SMase activation (Santana et al, 1995); in some cells, this may occur through upregulation of BCL-2 or BCL-XL (Hallahan et al, 1992a;Uckun et al, 1993). bFGF similarly protected endothelial cells against radiationinduced apoptosis, in part via activation of the PKC and MAPK pathways (Haimovitz-Friedman et al, 1994a;Verheij et al, 1996), but also via inhibition of SMasemediated generation of ceramide (Haimovitz-Friedman, unpublished).…”

Section: X-irradiationmentioning

confidence: 99%

Radiation and ceramide-induced apoptosis

2003

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“…Sphingomyelin and its hydrolysis product, ceramide levels were monitored by the method of Santana et al (1995) with slight modifications. A20 cells were labeled with 2 µCi/ml of [ 3 H]serine in the minimum essential medium for 18 h and treated with anti-Fas monoclonal antibody for various time periods.…”

Section: Sphingomyelin Hydrolysis Assaymentioning

confidence: 99%

Differential effects of Fas cross-linking on phospholipase D activation and related lipid metabolism in Fas-resistant A20 cells

Lim

Lee²,

Shin³

et al. 2002

Exp Mol Med

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A20 murine lymphoma cells undergoing Fas-mediated apoptosis showed increase in the activity of phospholipase D (PLD), which is involved in proliferative or mitogenic cellular responses. Using A20 cell lines that were resistant to Fas-induced apoptosis, we investigated the differential effects of Fas cross-linking on PLD activity and sphingolipid metabolism. The basal PLD activities in all of the selected three Fasresistant clones (#5, #8, and #11) were about 2~4 folds higher than that of wild type A20 cells. Among the PLD isoforms, PLD2 expression was increased in all of the selected Fas-resistant clones. The Fas downstream signaling events triggered by Fas cross-linking, including the activations of PLD, phosphatidylcholine-specific phospholipase C (PC-PLC), sphingomyelinase (SMase), the increase in diacylglycerol (DAG) and protein phosphorylation levels, and the translocation of protein kinase C to membrane were not changed in both of Fas-resistant clone #5 and #8. In contrast, Fas cross-linking stimulated the activity of PLD, PC-PLC, and SMase, translocation of PKC, and protein phosphorylation in Fas-resistant clone #11, similar to that of wild type cells. We also found that clone #11 had a different Fas sequence encoding Fas B which has been known to inhibit Fas-induced apoptosis. These findings suggest that increased PLD2 expression resulting in increased basal PLD activity and the blockade of Fas downstream signaling cascades may be involved to limit apoptosis induced by Fas cross-linking.

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Ceramide mediates tumor necrosis factor effects on P450-aromatase activity in cultured granulosa cells.

Cited by 56 publications

References 0 publications

Sphingosine 1-phosphate: a novel stimulator of aldosterone secretion

Sphingosine 1-phosphate: a novel stimulator of aldosterone secretion

Radiation and ceramide-induced apoptosis

Differential effects of Fas cross-linking on phospholipase D activation and related lipid metabolism in Fas-resistant A20 cells

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