Cerato-platanin protein is located in the cell walls of ascospores, conidia and hyphae of Ceratocystis fimbriata f. sp. platani

Boddi, Silvia

doi:10.1016/j.femsle.2004.03.001

Cited by 42 publications

(55 citation statements)

References 32 publications

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“…In contrast to these reported functions of cerato-platanin members, we have demonstrated that Sm1, produced by a beneficial microorganism T. virens, does not have toxic activity and instead is an effective elicitor of systemic resistance (Djonovic et al, 2006a). Very little is known about the role of these proteins in fungal development and physiology (Boddi et al, 2004). When a SM1 homolog from Leptosphaeria maculans, SP1, was disrupted, it was shown not to be crucial for pathogenicity; however, the effect of the SP1 deletion for fungal development has not been reported (Wilson et al, 2002).…”

Section: Possible Role Of Sm1 In T Virens Physiologycontrasting

confidence: 73%

A Proteinaceous Elicitor Sm1 from the Beneficial FungusTrichoderma virensIs Required for Induced Systemic Resistance in Maize

et al. 2007

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We have previously shown that the beneficial filamentous fungus Trichoderma virens secretes the highly effective hydrophobinlike elicitor Sm1 that induces systemic disease resistance in the dicot cotton (Gossypium hirsutum). In this study we tested whether colonization of roots by T. virens can induce systemic protection against a foliar pathogen in the monocot maize (Zea mays), and we further demonstrated the importance of Sm1 during maize-fungal interactions using a functional genomics approach. Maize seedlings were inoculated with T. virens Gv29-8 wild type and transformants in which SM1 was disrupted or constitutively overexpressed in a hydroponic system or in soil-grown maize seedlings challenged with the pathogen Colletotrichum graminicola. We show that similar to dicot plants, colonization of maize roots by T. virens induces systemic protection of the leaves inoculated with C. graminicola. This protection was associated with notable induction of jasmonic acidand green leaf volatile-biosynthetic genes. Neither deletion nor overexpression of SM1 affected normal growth or development of T. virens, conidial germination, production of gliotoxin, hyphal coiling, hydrophobicity, or the ability to colonize maize roots. Plant bioassays showed that maize grown with SM1-deletion strains exhibited the same levels of systemic protection as nonTrichoderma-treated plants. Moreover, deletion and overexpression of SM1 resulted in significantly reduced and enhanced levels of disease protection, respectively, compared to the wild type. These data together indicate that T. virens is able to effectively activate systemic disease protection in maize and that the functional Sm1 elicitor is required for this activity.

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Section: Possible Role Of Sm1 In T Virens Physiologycontrasting

confidence: 73%

A Proteinaceous Elicitor Sm1 from the Beneficial FungusTrichoderma virensIs Required for Induced Systemic Resistance in Maize

et al. 2007

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“…A trap technique was described previously for isolation of C. platani from soil and from infected wood (51). A serological assay has also been optimized to confirm the presence of the CP protein from C. platani ascospores and mycelium (52), but it never has been used for diagnostic purposes. A molecular approach based on qPCR to detect C. platani from artificially and naturally infected plane wood has been previously reported (53).…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

Luchi¹,

Ghelardini²,

Belbahri

et al. 2013

Appl Environ Microbiol

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Ceratocystis platani is the causal agent of canker stain of plane trees, a lethal disease able to kill mature trees in one or two successive growing seasons. The pathogen is a quarantine organism and has a negative impact on anthropogenic and natural populations of plane trees. Contaminated sawdust produced during pruning and sanitation fellings can contribute to disease spread. The goal of this study was to design a rapid, real-time quantitative PCR assay to detect a C. platani airborne inoculum. Airborne inoculum traps (AITs) were placed in an urban setting in the city of Florence, Italy, where the disease was present. Primers and TaqMan minor groove binder (MGB) probes were designed to target cerato-platanin (CP) and internal transcribed spacer 2 (ITS2) genes. The detection limits of the assay were 0.05 pg/l and 2 fg/l of fungal DNA for CP and ITS, respectively. Pathogen detection directly from AITs demonstrated specificity and high sensitivity for C. platani, detecting DNA concentrations as low as 1.2 ؋ 10 ؊2 to 1.4 ؋ 10 ؊2 pg/l, corresponding to ϳ10 conidia per ml. Airborne inoculum traps were able to detect the C. platani inoculum within 200 m of the closest symptomatic infected plane tree. The combination of airborne trapping and real-time quantitative PCR assay provides a rapid and sensitive method for the specific detection of a C. platani inoculum. This technique may be used to identify the period of highest risk of pathogen spread in a site, thus helping disease management.

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“…CP is a component of the cell wall of C. platani, but is also released during growth (Bernardi et al 2011;Boddi et al 2004;Scala et al 2004). The three-dimensional structure of this protein has been defined: CP has a double ψβ-barrel fold similar to that occurring in endoglucanases, in the plant defence protein barwin and in domain I of expansins (de Oliveira et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cerato-platanin shows expansin-like activity on cellulosic materials

Baccelli

Luti

Bernardi

et al. 2013

Appl Microbiol Biotechnol

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Cerato-platanin (CP) is a non-catalytic protein with a double =β-barrel fold located in the cell wall of the phytopathogenic fungus Ceratocystis platani. CP is released during growth and induces defence-related responses in plants. CP is also the first member of the "cerato-platanin family" (CPF) (Pfam PF07249). In the CPF, the molecular mechanism of action on plants and above all the biological role in fungal life are little-known aspects. However, an expansin-like function has recently been suggested concerning CP. Expansin-like proteins have the ability to act non-hydrolytically on cellulose. In the present work, the expansin-like activity of CP and Pop1, a CP family member, was investigated. Like expansins, CP and Pop1 were able to weaken filter paper in a concentration-dependent manner and without the production of reducing sugars. A metal-dependent polysaccharide monooxygenase-like activity was excluded. The optimum of activity was pH5.0, 38°C. CP was also able to cause fragmentation of the crystalline cellulose Avicel and the breakage and defibration of cotton fibres. However, the interaction did not involve a stable bond with the substrates and CP did not significantly enhance the hydrolytic activity of cellulase. On the other hand, CP and Pop1 bound quickly to chitin. We consider CP as a novel one-domain expansin-like protein. We propose a structural role for CP in the fungal cell wall due to the ability to bind chitin, and hypothesize a functional role in the interaction of the fungus with the plant for the weakening activity shown on cellulose.

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Cerato-platanin protein is located in the cell walls of ascospores, conidia and hyphae of Ceratocystis fimbriata f. sp. platani

Cited by 42 publications

References 32 publications

A Proteinaceous Elicitor Sm1 from the Beneficial FungusTrichoderma virensIs Required for Induced Systemic Resistance in Maize

A Proteinaceous Elicitor Sm1 from the Beneficial FungusTrichoderma virensIs Required for Induced Systemic Resistance in Maize

Rapid Detection of Ceratocystis platani Inoculum by Quantitative Real-Time PCR Assay

Cerato-platanin shows expansin-like activity on cellulosic materials

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