Cerebellar contribution to absence epilepsy

Akyüz, Enes; Ozenen, Cansu; Pinyazhko, O. R.; Poshyvak, Olesya; Godlevsky, L. S.

doi:10.1016/j.neulet.2021.136110

Cited by 4 publications

(6 citation statements)

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“…SWPs begin to appear on the cortical records of the two-month-old WAG/Rij rats, and their duration and frequency increase with age (16). The somatosensory cortex has an important role in triggering and generating of SWPs (17). The somatosensory cortex has typically six layers.…”

Section: Discussionmentioning

confidence: 99%

C-Met Receptors Deficiency Was Involved in Absence Seizures Development in WAG/Rij Rats

et al. 2023

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Background: A variety of receptors may be involved in the pathogenesis of absence seizures. The c-Met receptors have a critical role in modulating the GABAergic interneurons and creating a balance between excitatory and inhibitory neurotransmission, sensorimotor gating, and normal synaptic plasticity. Objectives: This study aimed to assess the changes of the c-Met receptor during the appearance of absence attacks in the experimental model of absence epilepsy. Methods: A total of 48 animals were divided into four groups of two- and six-month-old WAG/Rij and Wistar rats. Epileptic WAG/Rij rats showing SWP in electrocorticogram (ECoG) were included in the epileptic group. The two-month-old WAG/Rij rats as well as two- and six-month-old Wistar rats not exhibiting SWP in ECoG were selected as the non-epileptic. Gene (RT-PCR) and protein expression (western blotting) of c-Met receptors as well as c-Met protein distribution (immunohistochemistry) in the somatosensory cortex and hippocampus were assessed during seizure development of the absence attacks. Results: According to the study findings, a lower c-Met gene and protein expression, as well as a lower protein distribution, were observed in the hippocampus (P < 0.001, P < 0.05, and P < 0.001, respectively) and cortex (P < 0.01, P < 0.001 and P < 0.001, respectively) of the two-month-old WAG/Rij rats compared to the same-age Wistar rats. Moreover, the data revealed a reduction of hippocampal and cortical c-Met protein expression (P < 0.001, for both) in six-month-old WAG/Rij rats compared to two-month-old ones. Six-month-old WAG/Rij rats had a lower cortical c-Met gene (P < 0.05) and protein expression (P < 0.001) as well as lower hippocampal and cortical protein distribution (P < 0.05 and P < 0.001) than the same-age Wistar rats. Conclusions: In sum, the c-Met receptor was found to play a significant role in the development of absence epilepsy. This receptor, therefore, may have been considered as an effective goal for absence seizure inhibition.

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Section: Discussionmentioning

confidence: 99%

C-Met Receptors Deficiency Was Involved in Absence Seizures Development in WAG/Rij Rats

et al. 2023

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“…МІГУ-4 використовували у дозах 5,0 та 25,0 мг/кг, в/очер в 0,9 % фізіологічного розчину NaCl протягом 14 діб у щурів із сформованим діабетом. Зважаючи на встановлену виразну нейротропну активність МІГУ-4 [8], в якості препарату порівняння застосовували діазепам, який також демонструє високу протизапальну активність [10,11]. Діазепам (Calmpose, Ranbaxy Diagnostics, Індія) застосовували у дозах 0,5 та 1,5 мг/кг, в/очер протягом 14 діб (рис.1).…”

Section: модель діабетичної ретинопатії та групи спостереженняunclassified

Section: методи дослідженняunclassified

“…Для дії бензодіазепінових препаратів, зокрема при їх тривалому застосуванні, характерним є виникнення синдрому відміни, який проявляється зростанням тривожності [10,11]. З метою порівняння ефектів МІГУ-4 та діазепаму у дослідженні вивчали рухові порушення у тесті «відкритого поля», які є інформативними щодо наявності подібних проявів [10]. Синдром відміни препаратів оцінювали за показниками дослідження у «відкритому полі», яке здійснювали через 24 години з моменту останнього курсового введення препаратів.…”

Section: методи дослідженняunclassified

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Neurodegenrative changes in retina of rats with streptozotocin-induced diabetes under conditions of treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4)

Кресюн,

Аль-Надаві

2023

Oftalmol Zh

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Background: Diabetes is accompanied by neurodegenerative changes in the retina. The determination of retinal function parameters under conditions of experimental diabetes may allow for the development of new approaches to pharmacological vision correction. Purpose: To assess changes in the metabolic (lipid peroxidation and antioxidant defence) and functional characteristics (electroretinography (ERG)) of the retina in the rat with streptozotocin (STZ)-induced diabetes, treated with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) and a reference drug, diazepam. Material and Methods: Diabetes was induced intraperitoneally (i.p.) in Wistar male rats by streptozotocin (STZ 65 mg/kg). Four weeks thereafter, the rats received a two-week course of a daily dose of MIGU-4 (5.0 mg/kg or 25.0 mg/kg) or diazepam (0.5 mg/kg or 1.5 mg/kg). The electroretinogram (ERG) was obtained, the animals were euthanized and retinal malondialdehyde (MDA) levels and superoxide dismutase (SOD) and catalase (CAT) activity were determined. Results: In rats with untreated STZ-induced diabetes, the retinal MDA level was 3.71 times increased and CAT activity was 35% lower, compared to controls, whereas the SOD activity was half of the activity found in controls (р less 0.05). In diabetic animals treated with MIGU-4 i.p. at a 25.0-mg/kg daily dose, the retinal MDA level was 63.2% lower, whereas the CAT activity and SOD activity were 41.9% and 27.6%, respectively, higher, than in untreated diabetic rats (р less 0.05). In diabetic animals treated with diazepam i.p. at a 1.5-mg/kg daily dose, the retinal MDA level was 59.2% lower, whereas the CAT activity and SOD activity were 44.4% and 32.1%, respectively, higher, than in untreated diabetic rats (р less 0.05). In untreated diabetic rats, the ERG b-wave amplitude was 39.2% lower, ERG a-wave and b-wave latencies, 23.4% and 14.0%, respectively, higher, and the a-wave amplitude recovery rate, 38.8% lower than in controls (р less 0.05). The use of a 25.0-mg/kg daily dose of MIGU-4 resulted in a 29.7% and 33.9%, respectively, increase in the ERG b-wave amplitude and the a-wave amplitude recovery rate compared to untreated diabetic rats (р less 0.05). The use of a 1.5-mg/kg daily dose of diazepam resulted in a 25.0% and 30.0%, respectively, increase in the above ERG parameters compared to untreated diabetic rats (р less 0.05). In untreated diabetic rats, the number of squares crossed (SK) was 29.4% lower (р less 0.05); the number of central squares crossed (CSC), 52.1% lower (р less 0.05); the number of upright postures (UP), 34.2% lower; and the defecation bolus number, 32.4% higher than in controls (р less 0.05). After MIGU-4 (25-mg/kg) withdrawal, there were no more differences in SK, CSC, UP and defecation bolus numbers between treated diabetic rats and controls. In addition, the number of CSC was 4.27 times lower than in controls, and half of that in untreated diabetic animals (р less 0.05). In diabetic rats treated with diazepam, the number of UP was 2.56 times lower than in controls (р less 0.05), and 40.7% lower than in untreated diabetic rats (р less 0.05), whereas the defecation bolus number was 40.5% higher than in controls (р less 0.05). Conclusion: STZ-induced diabetes is accompanied by an impairment of the oxidant/ antioxidant balance in the retina with an increase in retinal MDA, decrease in retinal SOD and CAT activity, and ERG abnormalities such as reduced ERG amplitude and increased ERG latency. A course treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4) i.p. at a daily dose of 25.0 mg/kg provides for a decrease in retinal MDA and an increase in retinal SOD and CAT activity and ERG wave amplitude The withdrawal of a two-week treatment with MIGU-4 i.p. at a daily dose of 25.0 mg/kg decreased open-field anxiety-like behaviors, whereas the withdrawal of a two-week treatment with diazepam i.p. at a daily dose of 1.5 mg/kg increased open-field anxiety-like behaviors. The corrective effect of treatment with MIGU-4 corresponds to the corrective effect of treatment with diazepam.

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“…Penicillin‐induced epilepsy occurs due to the opening of voltage‐dependent calcium channels in the dendrites of the hippocampus and cortex, which causes a decrease in extracellular Ca 2+ concentration and an increase in intracellular Ca 2+ concentration. This results in excessive excitation and abnormal discharges of neurons, leading to seizures (Akyuz et al, 2021).…”

Section: Animal Model Of Epilepsy For Eit Studymentioning

confidence: 99%

Animal models for epileptic foci localization, seizure detection, and prediction by electrical impedance tomography

Wang

Zhu

Liang

et al. 2022

WIRES Cognitive Science

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Cerebellar contribution to absence epilepsy

Cited by 4 publications

References 75 publications

C-Met Receptors Deficiency Was Involved in Absence Seizures Development in WAG/Rij Rats

C-Met Receptors Deficiency Was Involved in Absence Seizures Development in WAG/Rij Rats

Neurodegenrative changes in retina of rats with streptozotocin-induced diabetes under conditions of treatment with niacin-oxy-ethylidene-diphosphonate germanate (MIGU-4)

Animal models for epileptic foci localization, seizure detection, and prediction by electrical impedance tomography

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