Cerebrospinal fluid amino acid concentrations in patients with viral and tuberculous meningitis.

Corston, R N; McGale, E.H.F.; Stonier, C.; Hutchinson, Edward; Aber, G. M.

doi:10.1136/jnnp.44.9.791

Cited by 15 publications

(5 citation statements)

References 7 publications

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“…The slightly elevated levels in patients B and C might have been caused by the seizures and coma they suffered. Increased CSF concentrations of glutamine have been reported in hepatic encephalopathy, liver disease, Reye's syndrome, respiratory acidosis, meningitis, and cerebral hemorrhage (Jaiken and Agrest, 1969;Hourani et al, 1971;Glasgow and Dhiensiri, 1974;Vergara et al, 1974;Bandoli et al, 1979;Corston et al, 1979Corston et al, , 1981Boeckx et al, 1980;Fishman, 1980;Cascino et al, 1982). The elevated levels seen in patient C could reflect the chemical pathology of Reye's syndrome.…”

Section: Discussionmentioning

confidence: 90%

High‐Resolution Proton Magnetic Resonance Analysis of Human Cerebrospinal Fluid

Petroff¹,

Yu²,

Ogino³

1986

Journal of Neurochemistry

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High‐resolution proton magnetic resonance spectroscopy was used to analyze human cerebrospinal fluid obtained from patients with several neurological problems. The major metabolites measured included glucose, lactate, glutamine, citrate, inositol, acetate, creatine, creatinine, β‐hydroxybutyrate, alanine, and pyruvate. A drug vehicle, propylene glycol, was also measured. Alterations in the cerebrospinal fluid of these metabolites provided information concerning metabolism of the brain. Magnetic resonance spectroscopy offered a simple and rapid means of assessing these and other exogenous and endogenous compounds in diseases affecting the nervous system.

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Section: Discussionmentioning

confidence: 90%

High‐Resolution Proton Magnetic Resonance Analysis of Human Cerebrospinal Fluid

Petroff¹,

Yu²,

Ogino³

1986

Journal of Neurochemistry

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“…Perturbations in amino acids in TBM cases are not a novel discovery as they have been observed previously in biochemical studies. In 1981 , Corston et al examined eleven viral meningitis and four TBM cases and reported that total amino acid concentrations in CSF were markedly higher in TBM than in viral meningitis. In 1998, Qureshi et al compared several neurochemical markers in CSF between 11 cases of viral meningitis and 12 of TBM.…”

Section: Introductionmentioning

confidence: 99%

Cerebrospinal Fluid Amino Acid Profiling of Pediatric Cases with Tuberculous Meningitis

Mason

Solomons

2017

Front. Neurosci.

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Background: In Africa, tuberculosis is generally regarded as persisting as one of the most devastating infectious diseases. The pediatric population is particularly vulnerable, with infection of the brain in the form of tuberculous meningitis (TBM) being the most severe manifestation. TBM is often difficult to diagnose in its early stages because of its non-specific clinical presentation. Of particular concern is that late diagnosis, and subsequent delayed treatment, leads to high risk of long-term neurological sequelae, and even death. Using advanced technology and scientific expertise, we are intent on further describing the biochemistry behind this devastating neuroinflammatory disease, with the goal of improving upon its early diagnosis.Method: We used the highly sensitive analytical platform of gas chromatography-mass spectrometry (GC-MS) to analyze amino acid profiles of cerebrospinal fluid (CSF) collected from a cohort of 33 South African pediatric TBM cases, compared to 34 controls.Results: Through the use of a stringent quality assurance procedure and various statistical techniques, we were able to confidently identify five amino acids as being significantly elevated in TBM cases, namely, alanine, asparagine, glycine, lysine, and proline. We found also in an earlier untargeted metabolomics investigation that alanine can be attributed to increased CSF lactate levels, and lysine as a marker of lipid peroxidation. Alanine, like glycine, is an inhibitory neurotransmitter in the brain. Asparagine, as with proline, is linked to the glutamate-glutamine cycle. Asparagine is associated with the removal of increased nitrites in the brain, whereas elevated proline coincides with the classic biochemical marker of increased CSF protein in TBM. All five discriminatory amino acids are linked to ammonia due to increased nitrites in TBM.Conclusion: A large amount of untapped biochemical information is present in CSF of TBM cases, of which amino acid profiling through GC-MS has potential in aiding in earlier diagnosis, and hence crucial earlier treatment.

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“…An extensive literature survey has revealed that a few studies have been reported in recent past that examined the concentration of amino acids in the CSF during bacterial [ 3 , 4 , 5 , 6 , 7 , 8 ] and viral meningitis [ 8 ]. Conversely, Molero-Luis et al [ 9 ] has recently reported the abnormal values of homovanillic acid (HVA) i.e., a neurologically important intermediate product of dopamine degradation, in CSF of children suspected with neurological disorders.…”

Section: Introductionmentioning

confidence: 99%

Cerebrospinal Fluid Concentrations of Biogenic Amines: Potential Biomarkers for Diagnosis of Bacterial and Viral Meningitis

Taj

Jamil

2018

Pathogens

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Catecholamine and serotonin are biogenic amines (BAs) that serve as neurotransmitters and play an important role in the regulation of cardinal functions that are mainly altered during central nervous system (CNS) infections. A total 92 samples of cerebrospinal fluid (CSF) were classified into 4 groups based on their etiology. In these samples, BAs/neurotransmitters i.e., dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5HIAA) were detected and quantified by high performance liquid chromatography with electrochemical detection (HPLC-EC) to determine the neurophysiology of the CNS infections by bacteria (Listeria monocytogenes (Lm) and Neisseria meningitidis (Nm)) and herpes simplex virus (HSV). CSF concentration of DA, DOPAC, HVA, and 5HIAA were found significantly elevated in all test cohorts. Present study highlights that the analysis of BAs is pivotal for the early diagnosis of bacterial and viral meningitis. In addition, coinfections of varied etiology can also be diagnosed by their quantification. Thus, BAs can serve as potential biomarkers of these CNS infections.

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Cerebrospinal fluid amino acid concentrations in patients with viral and tuberculous meningitis.

Cited by 15 publications

References 7 publications

High‐Resolution Proton Magnetic Resonance Analysis of Human Cerebrospinal Fluid

High‐Resolution Proton Magnetic Resonance Analysis of Human Cerebrospinal Fluid

Cerebrospinal Fluid Amino Acid Profiling of Pediatric Cases with Tuberculous Meningitis

Cerebrospinal Fluid Concentrations of Biogenic Amines: Potential Biomarkers for Diagnosis of Bacterial and Viral Meningitis

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