Cerebrospinal fluid lymphocytes from patients with multiple sclerosis do not increase immunoglobulin or measles antibody production after stimulation with pokeweed mitogen

Henriksson, Annemarie; Kam‐Hansen, Slavenka; Forsberg, Pia; Grandien, M

doi:10.1016/0165-5728(86)90071-8

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…Conversely, the poor response of in vitro B lymphocyte stimulation might be due to maximal in vivo activation of these cells thereby limiting further Ig production. This suggestion is supported by similar in vitro studies with cerebrospinal fluid lymphocytes from patients with multiple sclerosis, an inflammatory demyelinating disease of the central nervous system with immune reactivity for myelin antigens (Henriksson et al 1986). Lymphocytes from mdx mice (12 weeks onwards) also produced in vitro IgM antibody with lower reactivity for antigens (histone, myosin) known to be released from the lesioned tissue, rather than for unrelated antigens (MBP).…”

Section: Discussionmentioning

confidence: 69%

Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon‐γ production

Lagrota-Candido

Vasconcellos

Cavalcanti

et al. 2002

Int J Experimental Path

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Summary. Mdx mouse, the animal model of Duchenne muscular dystrophy, develops an X-linked recessive inflammatory myopathy with an apparent sustained capacity for muscle regeneration. We analysed whether changes in the skeletal muscle during myonecrosis and regeneration would correlate with functional alterations in peripheral lymphoid tissues. Here we show that during the height of myonecrosis, mdx mice display marked atrophy of peripheral lymph nodes and extensive muscle inflammation. In contrast, enlargement of draining lymph nodes with accumulation of CD4 CD44 , CD4 CD25 , CD8 CD44 T lymphocytes and type-2 B cells was consistently observed during amelioration of the muscle lesion. In addition, regeneration of the muscular tissue was accompanied by concomitant increase of immunoglobulin-secreting cells in regional lymph nodes and bone marrow. Double immunolabelling analysis revealed intense B cell proliferation and formation of germinal centre in the follicles of dystrophic regional lymph nodes. Furthermore, lymph node cells produced large amounts of IFN-g but not IL-4, IL-6 or IL-10 after in vitro mitogen stimulation with Concanavalin A. As these alterations occurred mainly during the recovery period, we suggested that local activation of the immune system could be an influence which mitigates the myonecrosis of muscular tissue in the mdx dystrophic mouse.

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Section: Discussionmentioning

confidence: 69%

Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon‐γ production

Lagrota-Candido

Vasconcellos

Cavalcanti

et al. 2002

Int J Experimental Path

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“…We wonder if perhaps their medium was somewhat stimulatory. It is in teresting that both Henriksson et al [1986] and Hauser et al [1985] observed that the PBL of MS patients 'tended to secrete more IgG in PWM-stimulated cultures than con trols' although in neither case was this signif icant. Similarly, Kelley et al [ 1981 ] found no differences in the amount of IgG secreted by PWM-stimulated PBMNC isolated from MS patients and normal controls but their sam ple size was small.…”

Section: In Vitro Igg Secretion In Msmentioning

confidence: 91%

“…Henriksson also studied PWM stimulation of CSF lymphocytes and found no response to PWM in the MS group, as opposed to the aseptic meningoencephalitis group, whose CSF lymphocytes secreted sig nificant concentrations of immunoglobulin into the supernatant. Henriksson et al [1986] suggest that the lack of response in the MS CSF lymphocytes is due to the fact that they were activated in vivo. This hy pothesis is further supported by their pre vious observation that the number of immu noglobulin-secreting cells is higher in the CSF than in the peripheral blood in MS (see earlier) [Henriksson et al, 1985].…”

Section: In Vitro Igg Secretion In Msmentioning

confidence: 97%

“…It was not determined if the excess help was the result of increased T helper cell activity or decreased T suppressor cell activity. Henriksson et al [1986] studied PWMinduced immunoglobulin secretion in MS patients, healthy controls and acute aseptic meningoencephalitis patients. There were no differences in the concentration of immuno globulin secreted in the supernatants of un stimulated or PWM-stimulated PBMNC cultures between the MS and the aseptic meningoencephalitis groups.…”

Section: In Vitro Igg Secretion In Msmentioning

confidence: 99%

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Cell-Mediated Immune Functions in Multiple Sclerosis

O’Gorman

Oger²

1987

Path Immunopathol Res

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“…Although CSF B cells have been shown to produce virus-specific antibodies in vitro (Henriksson 1986;Salmi 1989;Baig 1989), the clonal patterns of these B cells are not known. In this study we wanted to explore:…”

Section: Paper 1: Virus-specific Igg Antibodies Produced By Csf B Celmentioning

confidence: 99%

MS and clinically isolated syndromes: Shared specificity but diverging clonal patterns of virus‐specific IgG antibodies produced in vivo and by CSF B cells in vitro

Skorstad

Vandvik

Vartdal

et al. 2009

Euro J of Neurology

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Background: Intrathecal synthesis of oligoclonal IgG antibodies against measles virus (MeV), varicella zoster virus (VZV) and herpes simplex virus type‐1 (HSV‐1) is a characteristic feature multiple sclerosis (MS). Methods: We have used isoelectric focusing‐immunoblot to define the clonal patterns of IgG and of IgG antibodies to MeV, VZV and HSV‐1 in supernatants of in vitro cultures of peripheral blood lymphocytes (PBL) and cerebrospinal fluid (CSF) cells and in sera and CSF from three patients with MS and three patients with clinically isolated syndromes (CIS) suspective of demyelinating disease. Results: In vitro synthesis of IgG by PBL was not detected in any patient. In contrast, in vitro synthesis by CSF cells of oligoclonal IgG and oligoclonal IgG antibodies to one or two of the three viruses tested was observed in all six patients. The clonal patterns of the in vitro synthesized IgG and virus specific IgG differed to varying extent from those synthesized intrathecally in vivo. However, in each patient, the in vitro and in vivo intrathecally produced antibodies displayed specificity for the same viruses. The addition of B cell activating factor (BAFF) had no effect on the amounts or clonal patterns of either total IgG or virus‐specific IgG produced by CSF cells in vitro. Conclusion: Virus specific B cells capable of spontaneous IgG synthesis are clonally expanded in the CSF of patients with MS. The B‐cell repertoire in CSF samples is only partially representative of the intrathecal B‐cell repertoire.

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Cerebrospinal fluid lymphocytes from patients with multiple sclerosis do not increase immunoglobulin or measles antibody production after stimulation with pokeweed mitogen

Cited by 10 publications

References 26 publications

Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon‐γ production

Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon‐γ production

Cell-Mediated Immune Functions in Multiple Sclerosis

MS and clinically isolated syndromes: Shared specificity but diverging clonal patterns of virus‐specific IgG antibodies produced in vivo and by CSF B cells in vitro

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