Ceriporia lacerata Mycelium Culture Medium as a Novel Anti-Aging Microbial Material for Cosmeceutical Application

Kim, Jeong‐Hwan; An, Changhun; Hwang, Seong Deok; Kim, So Hyun

doi:10.3390/cosmetics8040101

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Notably, even though a decrease was assessed compared to young NHDF, viability higher than 100% was determined in old NHDF after treatment with pepper extracts. Hence, in line with the previous literature regarding a wide range of plant extracts [ 16 , 37 ], these findings support the notion that a 1 mg/mL concentration of VP extract was even able to trigger fibroblast cell proliferation [ 38 , 39 ].…”

Section: Discussionsupporting

confidence: 91%

Voghera Sweet Pepper: A Potential Ally against Oxidative Stress and Aging

Gola

Gaiaschi

Roda

et al. 2023

IJMS

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In the present study, the potential functional properties of the extracts from the edible part of Capsicum annuum L. var. Peperone di Voghera (VP) were studied. The phytochemical analysis revealed a high amount of ascorbic acid, paralleled by a low carotenoid content. Normal human diploid fibroblasts (NHDF) were chosen as the in vitro model models to investigate the effects of the VP extract on oxidative stress and aging pathways. The extract of Carmagnola pepper (CP), another important Italian variety, was used as the reference vegetable. The cytotoxicity evaluation was performed firstly, using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, while the VP potential antioxidant and antiaging activity was investigated by immunofluorescence staining focusing on specifically selected proteins. The MTT data revealed the highest cell viability at a concentration of up to 1 mg/mL. The immunocytochemical analyses highlighted an increased expression of transcription factors and enzymes involved in redox homeostasis (Nrf2, SOD2, catalase), improved mitochondrial functionality, and the up-regulation of the longevity gene SIRT1. The present results supported the functional role of the VP pepper ecotype, suggesting a feasible use of its derived products as valuable food supplements.

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Section: Discussionsupporting

confidence: 91%

Voghera Sweet Pepper: A Potential Ally against Oxidative Stress and Aging

Gola

Gaiaschi

Roda

et al. 2023

IJMS

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“…Young and old fibroblasts were treated for 24 h with Voghera pepper (VP) and control Carmagnola pepper (CP) extracts at a concentration of 1 mg/mL. This concentration was determined based on the results of the MTT assay performed in our previous study [ 20 ], as well as previous literature data concerning other natural extracts [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

A Lombard Variety of Sweet Pepper Regulating Senescence and Proliferation: The Voghera Pepper

De Luca,

Gola,

Azzalin

et al. 2024

Nutrients

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Aging and its related disorders are important issues nowadays and the first cause of this physio-pathological condition is the overproduction of ROS. Ascorbic acid is an antioxidant mediator and its anti-aging proprieties are well known. Our previous data demonstrated that Voghera sweet pepper (VP), a distinctive type of pepper cultivated in Italy, is particularly rich in ascorbic acid. Based on these data, the anti-aging effect mediated by extracts of the edible part of VP was evaluated on an in vitro model of both young and old Normal Human Diploid Fibroblasts (NHDF). Using phase contrast microscopy, we observed that VP may help cells in the maintenance of physiological morphology during aging. Cytofluorimetric analyses revealed that VP extracts led to an increase in DNA synthesis and percentage of living cells, linked to a consequent increase in mitotic events. This hypothesis is supported by the enhancement of PCNA expression levels observed in old, treated fibroblasts, corroborating the idea that this extract could recover a young phenotype in adult fibroblasts, confirmed by the study of p16 and p53 expression levels and TEM analyses. Based on these results, we may suppose that VP can lead to the partial recovery of “young-like” phenotypes in old fibroblasts.

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“…The natural plant materials used in this study were Helianthus annus seeds, Perilla frutescens, Prunus armeniaca, Paeonia lactiflora, Morus mongolica (Bureau) C. K. Schneid, Angelica gigas, Sophora flavescens, Gardenia jasminoides, Saururus chinensis, Houttuynia cordata, Acanthopanax cordata, Polygala tenuifolia, and Cimicifuga racemosa. The characteristics of each plant are described in the Supplementary Materials of previously reported studies [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway

et al. 2023

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In this study, we aimed to develop natural and/or functional materials with antioxidant and anti-inflammatory effects. We obtained extracts from natural plants through an oil and hot-water extraction process and prepared an extract composite of an effective unsaturated fatty acid complex (EUFOC). Furthermore, the antioxidant effect of the extract complex was evaluated, and the anti-inflammatory effect was explored by assessing its inhibitory effect on nitric oxide production through its HA-promoting effect. We conducted a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay to evaluate the cell viability of the EUFOC, and the results showed that EUFOC was not cytotoxic at the test concentrations. In addition, it showed no endogenous cytotoxicity in HaCaT (human keratinocyte) cells. The EUFOC showed excellent 1,1-diphenyl-2-picrylhydrazyl- and superoxide-scavenging abilities. Moreover, it exerted an inhibitory effect on NO production at concentrations that did not inhibit cell viability. The secretion of all the cytokines was increased by lipopolysaccharide (LPS) treatment; however, this was inhibited by the EUFOC in a concentration-dependent manner. In addition, hyaluronic acid content was markedly increased by the EUFOC in a dose-dependent manner. These results suggest that the EUFOC has excellent anti-inflammatory and antioxidant properties, and hence, it can be used as a functional material in various fields.

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Ceriporia lacerata Mycelium Culture Medium as a Novel Anti-Aging Microbial Material for Cosmeceutical Application

Cited by 5 publications

References 33 publications

Voghera Sweet Pepper: A Potential Ally against Oxidative Stress and Aging

Voghera Sweet Pepper: A Potential Ally against Oxidative Stress and Aging

A Lombard Variety of Sweet Pepper Regulating Senescence and Proliferation: The Voghera Pepper

Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway

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