Cerium ions immobilized magnetic graphite nitride decorated with L-Alanyl-L-Glutamine as new chelator for enrichment of phosphopeptides

Jiang, Dandan; Wu, Siyu; Lv, Siqi; Qi, Ruixue; Li, Yangyang; Liu, Jinghai

doi:10.1007/s00604-023-06033-1

Cited by 1 publication

(1 citation statement)

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“…A549 cell proteins were extracted by incubating A549 cells in a lysis buffer. 29 The proteins were added to a urea (2 M)/NH 4 HCO 3 solution (50 mM) and reduced by dithiothreitol (10 mM) at 56 °C for 0.5 h. Then, the proteins were carboxymethylated with iodoacetamide (25 mM) for 0.5 h. Finally, trypsin was added to the solution and digested at 37 °C for 16 h.…”

Section: Sample Preparationmentioning

confidence: 99%

Cobalt Phthalocyanine-Modified Magnetic Metal–Organic Frameworks for Specific Enrichment of Phosphopeptides

Jiang,

Wu,

et al. 2024

ACS Biomater. Sci. Eng.

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For mass spectrometry (MS)-based phosphoproteomics studies, sample pretreatment is an essential step for efficient identification of lowabundance phosphopeptides. Herein, a cobalt phthalocyanine-modified magnetic metal−organic framework (MOF) (Fe 3 O 4 @MIL-101-CoPc) was prepared and applied to enrich phosphopeptides before MS analysis. Fe 3 O 4 @ MIL-101-CoPc exhibited an excellent magnetic response (74.98 emu g −1 ) and good hydrophilicity (7.75°), which were favorable for the enrichment. Fe 3 O 4 @ MIL-101-CoPc showed good enrichment performance with high selectivity (1:1:5000), sensitivity (0.1 fmol), reusability (10 circles), and recovery (91.3%). Additionally, the Fe 3 O 4 @MIL-101-CoPc-based MS method was able to successfully detect 827 phosphopeptides from the A549 cell lysate, demonstrating a high enrichment efficiency (89.3%). This study promotes the application of postfunctionalized MOFs for phosphoproteomics analysis.

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Section: Sample Preparationmentioning

confidence: 99%