Cerium oxide nanoparticles inhibit lipopolysaccharide induced MAP kinase/NF-kB mediated severe sepsis

Abstract

The life threatening disease of sepsis is associated with high mortality. Septic patient survivability with currently available treatments has failed to improve. The purpose of this study was to evaluate whether lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction can be prevented by cerium oxide nanoparticles (CeO2NPs) treatment in male Sprague Dawley rats. Here we provide the information about the methods processing of raw data related to our study published in Biomaterials (Selvaraj et al., Biomaterials, 2015, In press) and Data in Brief (Selvaraj et al., Data in Brief, 2015, In Press). The data present here provides confirmation of cerium oxide nanoparticle treatments ability to prevent the LPS induced sepsis associated changes in physiological, blood cell count, inflammatory protein and growth factors in vivo. In vitro assays investigation the treated of macrophages cells with different concentrations of cerium oxide nanoparticle demonstrate that concentration of cerium oxide nanoparticles below 1 µg/ml did not significantly influence cell survival as determined by the MTT assay.

Inhibition of MAP kinase/NF-kB mediated signaling and attenuation of lipopolysaccharide induced severe sepsis by cerium oxide nanoparticles

“…Animals were prepared for experiments as detailed in the Selvaraj et al [16] and were randomly assigned to one of four groups. The control group (n = 6) received 1.5 ml of endotoxin free water by i.p.…”

“…Proteins samples were prepared from the liver for immunoblotting as detailed in Selvaraj et al [16]. Liver cell apoptosis was assessed using a transferase-mediated dUTP nick-end labeling (TUNEL) kit (Roche Applied Science, Indianapolis, IN) as described in Selvaraj et al [16].…”

“…Liver cell apoptosis was assessed using a transferase-mediated dUTP nick-end labeling (TUNEL) kit (Roche Applied Science, Indianapolis, IN) as described in Selvaraj et al [16]. …”

“…The cytotoxic and protective effect of CeO 2 nanoparticles against LPS challenge was determined by the MTT assay as described in Selvaraj et al [16]. CeO 2 nanoparticle uptake by the macrophage cells was estimated by inductively coupled plasma-mass spectrometry (ICP-MS) at Elementary Analysis Inc (Lexington, Kentucky, USA) as described previously [20].…”

“…Nitrite production was assayed using the Griess reaction kit from Cayman Chemical Company (Ann Arbor, Michigan, USA) as described by the manufacturer. The concentration of TNF-α, IL-6, IL-1β and HMGB1 in the media was measured by ELISA reagent kits as described in Selvaraj et al [16]. …”