Cerrena unicolor Laccases, Genes Expression and Regulation of Activity

Abstract

A white rot fungus Cerrena unicolor has been identified as an important source of laccase, unfortunately regulation of this enzyme genes expression is poorly understood. Using 1D and 2D PAGE and LC-MS/MS, laccase isoenzymes were investigated in the liquid filtrate of C. unicolor culture. The level of expression of laccase genes was measured using qPCR. The elevated concentrations of copper and manganese in the medium caused greatest change in genes expression and three laccase transcripts were significantly affected after culture temperature was decreased from 28 to 4 °C or increased to 40 °C. The small differences in the PAGE band intensities of individual laccase proteins were also observed, indicating that given compound affect particular laccase’s transcript. Analyses of laccase-specific activity, at all tested conditions, showed the increased activities as compared to the control, suggesting that enzyme is regulated at the post-translational stage. We observed that the aspartic protease purified from C. unicolor, significantly stimulate laccase activity. Moreover, electrochemical analysis of protease-treated laccase sample had 5 times higher redox peaks. The obtained results indicate that laccases released by C. unicolor are regulated at transcriptional, translational, and at the post-translational steps of gene expression helping fungus adapt to the environmental changes.

Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613

“…5A , laccase activity decreased as the concentration of additive GSH increased, suggesting a dose-dependent negative regulation of laccase production by GSH. Previous studies have explored the effects of phenolic compounds (such as ferulic acid and veratric acid) and metal ions (such as Cu 2+ , Mn 2+ , and Cd 2+ ) on fungal laccase production ( 23 , 48 – 50 ). This is the first evidence demonstrating the inhibitory effect of oligopeptide GSH on fungal laccase activity.…”

Improved laccase production by Trametes versicolor using Copper-Glycyl-L-Histidyl-L-Lysine as a novel and high-efficient inducer

“…Therefore, the high-efficient absorption, accumulation and utilization of Cu could be one of the reasons for the good laccase expression induced by GHK-Cu. In fact, the promoter region of laccase gene may contain elements responding to Cu 2+ regulation, which regulated laccase gene expression in a strain-dependent manner ( Degerli et al, 2019 ; Pawlik et al, 2021 ). It has been proved that the increase in laccase gene expression level contributed to the increase in laccase production ( Rodrigues et al, 2019 ).…”

Laccase produced by Coriolopsis trogii and Cerrena unicolor with the mixed of metal ions and lignocellulosic materials

“…Previous studies had indicated excellent laccase secretion ability of Cerrena unicolor (Jamroz et al 2004;Rola et al 2013;Kachlishvili et al 2014Kachlishvili et al , 2021Pawlik et al 2021;Zhang et al 2021). There are also many studies on the effect of lignocellulose materials on laccase activity from C. unicolor.…”