Certification of a fish otolith reference material in support of quality assurance for trace element analysis

Sturgeon, Ralph E.; Willie, Scott; Yang, Lu; Greenberg, Ronald I.; Spatz, R; Chen, Z.; Scriver, Christine; Clancy, Vincent; Lam, Joseph W. H.; Thorrold, Simon R.

doi:10.1039/b503655k

Cited by 115 publications

(84 citation statements)

References 12 publications

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“…Instrument blanks of 2% HNO 3 were run at the beginning, middle, and end of a block of 10 otoliths, and were linearly interpolated. Dissolved otolith certified reference material (CRM) (Yoshinaga et al 2000) was used to correct for instrument mass bias (Rosenthal et al 1999), and instrument precision was assessed using a second type of otolith CRM (Sturgeon et al 2005). Detection limits were calculated as 3 standard deviations of the blanks that were run throughout the analyses (n = 219).…”

Section: Methodsmentioning

confidence: 99%

Chemical signatures in the otoliths of a coastal marine fish, Menidia menidia, from the northeastern United States: spatial and temporal differences

Clarke

Walther²,

Munch

et al. 2009

Mar. Ecol. Prog. Ser.

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Knowledge of population structure in marine systems is fundamental to effective management and conservation. The geochemical signature of otoliths may provide a promising natural tag for quantifying population structure in marine fishes. However, the spatial scale at which chemical signatures differ among species from different environments and with different life histories is not yet clear. We examined chemical signatures in the otoliths of juvenile Menidia menidia, a ubiquitous nearshore marine species found along the east coast of North America that undergoes offshore winter migration. Specimens were collected from 16 locations in 2003 and from 9 locations in 2004 between New Jersey and Maine. Otolith geochemistry was analyzed using laser ablation inductively coupled plasma-mass spectrometry and isotope ratio mass spectrometry. Juvenile fish showed significant site-specific differences and were assigned to natal sites with 70% (2003) and 77% (2004) average cross-validated classification accuracies based on elemental (Mg, Mn, Sr, Ba, Cu, and Pb relative to Ca) and isotope (δ 13 C, δ 18 O) ratios using quadratic discriminant function analysis. Geochemical signatures showed significant interannual variation, suggesting natural tags are year-class specific. Results suggest enough heterogeneity exists in marine chemical signatures to track movements and determine whether M. menidia return to their natal, nearshore location upon return from winter migration. Because M. menidia show evidence of local adaptation, knowledge of this winter migration will have evolutionary and ecological implications. Geochemical signatures of otoliths will likely serve as a useful tool in species with similar life histories.

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Section: Methodsmentioning

confidence: 99%

Chemical signatures in the otoliths of a coastal marine fish, Menidia menidia, from the northeastern United States: spatial and temporal differences

Clarke

Walther²,

Munch

et al. 2009

Mar. Ecol. Prog. Ser.

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“…To correct for mass bias and instrument drift, standards were run periodically during the analyses. For shell analyses, the solutionbased standards included an aragonitic otolith reference material (Yoshinaga et al 2000) and the certified reference material (CRM) FEBS-1 (Sturgeon et al 2005 Pb in low-resolution mode. These elements were chosen because of their previous use in successful elemental tagging studies (Kalish 1989, Elsdon & Gillanders 2002, Zacherl et al 2003b, Bath Martin & Thorrold 2005.…”

Section: Methodsmentioning

confidence: 99%

Temperature and salinity effects on elemental uptake in the shells of larval and juvenile softshell clams Mya arenaria

Strasser¹,

Mullineaux²,

Thorrold³

2008

Mar. Ecol. Prog. Ser.

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The chemical composition of biogenic carbonate has great potential to serve as a natural tag in studies of marine population connectivity. Yet the degree to which carbonate chemistry reflects ambient water composition may be influenced by environmental parameters, physiology, and uptake kinetics. We explored the effects of temperature and salinity on the uptake of elements into shells of larval and juvenile softshell clams Mya arenaria. Clams were reared under controlled conditions using combinations of temperatures (15, 20, and 24°C) and salinities (22 and 30 ‰) commonly encountered in their natural habitat. We analyzed the ratios of a suite of elements (Mg, Mn, Sr, Ba and Pb) to Ca in seawater and shells using solution-based inductively coupled plasma-mass spectrometry (ICP-MS). Elemental ratios were translated into discrimination coefficients (D element ) to account for water chemistry variability among treatments. For larval shell, we found that D Mn was lower in the low-temperature treatment than at higher temperatures, had mixed results for correlations with salinity, and exhibited an interactive effect between salinity and temperature. We also found that D Ba of larval shell was higher in the 15°C treatment than at the other 2 temperatures. In juvenile shell, we found a temperature effect for D Mn , however the exact relationship was unclear because D Mn was higher in the mid-temperature treatment than either the low-or high-temperature treatments. D Sr was negatively correlated with salinity in juveniles, with evidence of an interactive effect for temperature and salinity. D Ba and D Pb were both higher in juveniles in the low-temperature treatment than in the 2 higher temperatures. When discrimination coefficients significantly differed based on ANOVA, we used post hoc comparisons to further explore the effects of temperature and salinity. Correlation analyses showed that uptake differed significantly between larval and juvenile M. arenaria shell for all elements, with no predictable relationship in shell uptake between the 2 stages except for D Ba . All of the elements examined in this study have the potential to be useful in tagging studies where geographic variability in temperature, salinity or elemental concentrations exists, although caution should be used to ensure any biological interactions with these variables are accounted for in data interpretation.KEY WORDS: Mya arenaria · Bivalve shell · Elemental uptake · Discrimination coefficients · Temperature · Salinity · Larva · Biogenic carbonate Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 370: [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169] 2008 (and therefore its role in connectivity) is challenging due to small larval sizes, rapid dilution rates, and high larval mortality (Thorson 1950(Thorson , 1966.In recent years, the use of artificial and natural tags to track marine larvae has been explored (e.g. Thorrold et al. 2002, Levin 2006. One type of natur...

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“…Laser conditions were set to a frequency of 10 Hz and 60% energy. The typical Certified Reference Materials (CRMs) were used (Yoshinaga et al, 2000;Sturgeon et al, 2005), and sampled using the same instrument conditions at the beginning and end of each working session and after every 20 LA-spots. LA-ICPMS output data were processed using Glitter software (GEMOC, Macquarie University) to obtain element concentrations (µg Me /g otolith ) based on natural isotope ratios and using Ca 43 as an internal standard.…”

Section: Otolith Chemical Analysesmentioning

confidence: 99%

Daily otolith growth and ontogenetic geochemical signatures of age-0 anchovy (Engraulis encrasicolus) in the Gulf of Cádiz (SW Spain).

et al. 2014

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The European anchovy fishery in the Gulf of Cádiz (ICES Division IXa South) is largely influenced by age-0 individuals. Knowledge of young-of-the-year-growth dynamics is crucial for management, yet data on daily growth are lacking in the area. Linking growth patterns to the environment requires information on habitat occupancy through ontogeny of the fish that reach the fishery, as anchovy use different areas of the Gulf and the Guadalquivir Estuary throughout development. We describe the growth dynamics of age-0 anchovy through otolith microstructure analysis, and couple these data with data on microchemical signals in the otoliths to shed light into habitat use and growth dynamics in the area. Age-0 anchovy captured in September 2011 in the Gulf ranged from 3 to 6 months old for similar sizes, with average growth rates varying twofold. Individual non-linear growth curves showed that maximum otolith growth was positively correlated with the date of spawing. There was no correlation between growth parameters and body length or condition (Fulton K) at capture. The strontium:calcium (Sr/Ca) and magnesium:calcium (Mg/Ca) ratios were significantly lower at the edge of the otolith (approximately the age of capture) than at ages corresponding to larval and early juveniles (<60 days old), but values fell within typical estuarine-dwelling species values. The barium:calcium ratio (Ba/Ca) increased significantly at the edge of the otolith, which possibly resulted from residing in highly productive coastal waters or from ontogenetic effects. The variance in otolith elemental ratios was larger at otolith back-calculated ages around 50 days old, an age that coincides with the presumed closer dependence of estuarine waters. Our data are a first step towards understanding the contribution of the estuarine system to the anchovy fishery in the Gulf. The limitations of the approach and future steps are discussed.

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Certification of a fish otolith reference material in support of quality assurance for trace element analysis

Cited by 115 publications

References 12 publications

Chemical signatures in the otoliths of a coastal marine fish, Menidia menidia, from the northeastern United States: spatial and temporal differences

Chemical signatures in the otoliths of a coastal marine fish, Menidia menidia, from the northeastern United States: spatial and temporal differences

Temperature and salinity effects on elemental uptake in the shells of larval and juvenile softshell clams Mya arenaria

Daily otolith growth and ontogenetic geochemical signatures of age-0 anchovy (Engraulis encrasicolus) in the Gulf of Cádiz (SW Spain).

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