Certification of three mussel tissue standard reference materials (SRM) for methylmercury and total mercury content

Donais, Mary Kate; Saraswati, Rajananda; Mackey, Elizabeth A.; Demiralp, R.; Porter, Barbara J.; Vangel, Mark; Levenson, Mark; Mandic, V.; Azemard, Sabine; Horvat, Milena; May, K.; Emons, Hendrik; Wise, Stephen A.

doi:10.1007/s002160050441

Cited by 26 publications

(9 citation statements)

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“…The digested samples were diluted to 50 ml with Milli-Q water. Mussel tissue corresponding to the standard reference material MIST, 1976(Donais et al, 1997 was processed and analysed as a control sample, using the same protocol described above. The purity of the chemicals used was checked by analysis of appropriate blanks.…”

Section: Metal Contaminationmentioning

confidence: 99%

The Paracentrotus lividus populations from the northern Moroccan Atlantic coast: growth, reproduction and health condition

Bayed¹,

Quiniou

Benrha

et al. 2005

J. Mar. Biol. Ass.

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The condition of three intertidal Paracentrotus lividus populations, which inhabit an area between Rabat and Casablanca (Morocco) known by its high wave energy and its substantial anthropogenic disturbances were described. The growth rate of the juvenile sea urchins in this intertidal zone was low compared with more sheltered populations. A sharp increase in the gonad index was observed between January and March at the beginning of a period of algal production, and was concomitant with increases in seawater temperature and food consumption rate. This gonad index increase was followed by a single spawning period occurring between March and June, probably triggered by the phytoplankton bloom induced by upwelling conditions. Larval development in all three populations was severely inhibited and could be explained, in part, by high and generalized Cu contamination throughout the region. Specific contamination of particular populations by Pb or Hg was attributed to local industries and the incomplete dispersion of pollutants in spite of the high hydrodynamics along the Moroccan Atlantic coast. One of the three populations studied showed strong indications of abnormal development; the degradation of its condition appeared to be due to the presence of a wadi (temporary river) which can seasonally reduce the salinity, directly affecting the sea urchin physiology and indirectly enhancing the metal toxicity.

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Section: Metal Contaminationmentioning

confidence: 99%

The Paracentrotus lividus populations from the northern Moroccan Atlantic coast: growth, reproduction and health condition

Bayed¹,

Quiniou

Benrha

et al. 2005

J. Mar. Biol. Ass.

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“…The temperature was an important factor that would influence the extraction efficiency of mercury compounds. 25 In this experiment, we chose 0 C, room temperature (about 19.8 C), 45 and 70 C to investigate whether satisfactory recoveries of MeHg and EtHg could be obtained. Figure S7 shows the extraction recoveries of MeHg and EtHg from sewage sludge samples using acidic KBr/CuSO4 as the extractant for different ultrasonic times at different temperatures.…”

Section: Changing the Ultrasonic Extraction Temperaturementioning

confidence: 99%

Rapid Determination of Mercury Species in Sewage Sludge by High-Performance Liquid Chromatography On-line Coupled with Cold-Vapor Atomic-Fluorescence Spectrometry after Ultrasound-assisted Extraction

Gao

Liu

2011

ANAL. SCI.

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So far, no research has been focused on the determination of organomercuries in sewage sludge. Here, an ultrasonic extraction method for the rapid determination of methylmercury (MeHg) and ethylmercury (EtHg) in sewage sludge after ultrasound-assisted extraction is proposed. Using TMA (tetramethylammonium hydroxide) as the extractant with 3.0 g copper powder, ultrasonic extraction for 30 min at 70 C demonstrated to be highly efficient, and was shown a satisfied extraction efficiency for MeHg and EtHg from sewage sludge samples. Determination of mercury species was carried out by high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry (HPLC-CV-AFS). The obtained results from quality control samples were excellent. The proposed method was also validated by the application to five real samples. The results showed that the developed methodology was practicable and highly reliable. Due to the high toxicity of organomercuries and huge amounts of sewage sludge discharged every year, people should pay particular attention to pollutions from sewage sludge.

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“…Procedures for sample solubilisation. Three methods taken from the literature were tested and optimised for extraction of organic mercury: Saponification (KOH/MeOH) 9,18 . Addition of 3 ml of a 25% (w/v) potassium hydroxide (KOH) solution in methanol and ultrasonication at 70 °C for 3 h. After cooling, neutralisation with 3 ml 50% (v/v) HCl was required.…”

Section: Procedures For Mercury Extractionmentioning

confidence: 99%

Cold vapour atomic fluorescence spectrometry and gas chromatography-pyrolysis-atomic fluorescence spectrometry for routine determination of total and organometallic mercury in food samples

Ebdon

Foulkes

Roux

et al. 2002

Analyst

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Two procedures have been investigated for the quantification of the different forms of mercury in food. A two-stage procedure has been developed to determine firstly total inorganic and organometallic species, and then the full separation of all organomercury species. The procedure involves solubilisation of the samples using alkaline extractions or enzymolysis, followed by the extraction of organic mercury in an organic solvent, preferably a mixture of dichloromethane and hexane (3:2). For the total organic mercury determination, the organic extract is analysed for "total" mercury after nitric acid/peroxide digestion, evaporation of the solvent and detection by cold vapour-atomic fluorescence spectrometry. Full organomercury speciation requires a clean-up step before analysis of the final extract in dichloromethane by gas chromatography coupled to a pyrolyser and an atomic fluorescence detector (GC-pyro-AFS). A detection limit of 6 ng l-1, and reproducibility of 2% was achieved for the CV-AFS method; GC-pyro-AFS yielded 200 ng l-1 and 5% for detection limit and coefficient of variation, respectively. Both procedures were validated with the use of various certified reference materials over a wide range of mercury concentrations, and by spiking experiments. The validated methods were tested successfully on a wide range of commercially available food samples.

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Certification of three mussel tissue standard reference materials (SRM) for methylmercury and total mercury content

Cited by 26 publications

References 0 publications

The Paracentrotus lividus populations from the northern Moroccan Atlantic coast: growth, reproduction and health condition

The Paracentrotus lividus populations from the northern Moroccan Atlantic coast: growth, reproduction and health condition

Rapid Determination of Mercury Species in Sewage Sludge by High-Performance Liquid Chromatography On-line Coupled with Cold-Vapor Atomic-Fluorescence Spectrometry after Ultrasound-assisted Extraction

Cold vapour atomic fluorescence spectrometry and gas chromatography-pyrolysis-atomic fluorescence spectrometry for routine determination of total and organometallic mercury in food samples

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