Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins

Almeida, Ana Margarida; Queiroz, João A.; Sousa, Fani; Sousa, Ângela

doi:10.1016/j.drudis.2019.07.011

Cited by 72 publications

(55 citation statements)

References 102 publications

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“…phase check point and then triggering abnormal chromosome replication. Suppressing the action of E6 and E7 is a key approach for cervical cancer therapy (6,18).…”

Section: Resveratrol Inhibits the Progression Of Cervical Cancer By Smentioning

confidence: 99%

Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes

Sun

Xie

et al. 2020

Int J Mol Med

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Resveratrol is a representative polyphenol of diet-derived putative cancer chemopreventive agents, which have attracted increasing interest in the cancer chemoprevention community. The inhibition of the action of human papillomavirus (HPV) E6 and E7 has been considered a key approach for cervical cancer therapy. Resveratrol has been shown to induce the apoptosis, and reduce both the viability and mitotic index of a number of cancer cell lines, including human cervical cancer cells. In the present study, it was confirmed that resveratrol inhibited the HPV E6 mRNA, HPV E6 protein and phosphorylated retinoblastoma protein (p-pRb1) levels, and increased the p53 protein levels in HeLa and Ca Ski cells, as well as in subcutaneous tumor tissue grown from HeLa cells. High-risk HPV uses a bicistronic RNA to control E6 and E7 genes simultaneously. On the whole, the present study demonstrates that resveratrol inhibits cervical cancer development by suppressing the transcription and translation of E6 and E7, and also by promoting the apoptosis and G1/S phase transition arrest. These findings may provide the basis for the development of resveratrol as a candidate for cervical cancer therapy.

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“…phase check point and then triggering abnormal chromosome replication. Suppressing the action of E6 and E7 is a key approach for cervical cancer therapy (6,18).…”

Section: Resveratrol Inhibits the Progression Of Cervical Cancer By Smentioning

confidence: 99%

Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes

Sun

Xie

et al. 2020

Int J Mol Med

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“…Upon HPV infection, E6 and E7 oncoproteins begin to express and regulate downstream gene expressions, thus contributing to continuous cell proliferation to malignant transformation. 2 There are about 500,000 women get CC and 260,000 die from it every year. 3 Although the treatment technology of CC has been greatly improved, the 5-year overall survival rate is still poor and metastatic CC is incurable.…”

Section: Introductionmentioning

confidence: 99%

<p>LncRNA SNHG7 Functions as an Oncogene in Cervical Cancer by Sponging miR-485-5p to Modulate JUND Expression</p>

Zhao¹,

Zhang²,

Long³

et al. 2020

OTT

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Background: Long non-coding RNA (LncRNA) SNHG7 is involved in the development of multiple cancers. However, its role in cervical cancer (CC) has not been elucidated. This study aimed to explore the function of SNHG7 in CC progression and the underlying mechanisms. Materials and Methods: The expression levels of SNHG7 and miR-485-5p in CC tissues and cell lines were measured by qPCR. Functional experiments including CCK-8 assay, wound healing assay, transwell assay, flow cytometry, Western blot, luciferases reporter assay and immunoprecipitation (RIP) were performed to explore the SNHG7/miR-485-5p/JUND pathway. Additionally, in vivo study was carried out by establishing tumor xenograft models. Results: We found that SNHG7 was markedly enhanced in CC tissues and cell lines, and associated with poor clinical characteristics. In vitro, knockdown of SNHG7 inhibited CC cell proliferation, migration and invasion, as well as aggravated cell apoptosis. As to mechanism investigation, rescue experiments revealed that miR-485-5p inhibitor could partially reverse the effects on CC cells induced by SNHG7 knockdown. SNHG7 upregulated JUND expression via miR-485-5p. Moreover, tumor xenograft models were established to confirm the findings in vivo. Conclusion: SNHG7 promoted CC progression through miR-485-5p/JUND axis. The SNHG7/miR-485-5p/JUND pathway might provide a novel therapeutic target for CC treatment.

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“…However, HPVs interact with many other cancer-relevant pathways, even in a p53- and/or pRb-independent way. In addition, these oncoproteins may deregulate intracellular microRNA (miRNA) networks, and many HPV-altered miRNAs have been linked to carcinogenesis [ 29 ]. E6/E7 oncoproteins represent an ideal set of targets for a therapeutic vaccine against HPV-associated cancer because these proteins not only induce tumorigenesis but also are constitutively expressed in HPV-infected pre-malignant and malignant cells.…”

Section: Hpv Carcinogenesismentioning

confidence: 99%

Plant-Derived Natural Compounds in Genetic Vaccination and Therapy for HPV-Associated Cancers

Franconi

Massa

Paolini³

et al. 2020

Cancers

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Antigen-specific immunotherapy and, in particular, DNA vaccination provides an established approach for tackling human papillomavirus (HPV) cancers at different stages. DNA vaccines are stable and have a cost-effective production. Their intrinsic low immunogenicity has been improved by several strategies with some success, including fusion of HPV antigens with plant gene sequences. Another approach for the control of HPV cancers is the use of natural immunomodulatory agents like those derived from plants, that are able to interfere in carcinogenesis by modulating many different cellular pathways and, in some instances, to reduce chemo- and radiotherapy resistance of tumors. Indeed, plant-derived compounds represent, in many cases, an abundantly available, cost-effective source of molecules that can be either harvested directly in nature or obtained from plant cell cultures. In this review, an overview of the most relevant data reported in literature on the use of plant natural compounds and genetic vaccines that include plant-derived sequences against HPV tumors is provided. The purpose is also to highlight the still under-explored potential of multimodal treatments implying DNA vaccination along with plant-derived agents.

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Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins

Cited by 72 publications

References 102 publications

Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes

Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes

<p>LncRNA SNHG7 Functions as an Oncogene in Cervical Cancer by Sponging miR-485-5p to Modulate JUND Expression</p>

Plant-Derived Natural Compounds in Genetic Vaccination and Therapy for HPV-Associated Cancers

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