Cervical cancer cells (HeLa) response to photodynamic therapy using a zinc phthalocyanine photosensitizer

Hodgkinson, Natasha; Kruger, Cherie Ann; Mokwena, Mpho Gift; Abrahamse, Heidi

doi:10.1016/j.jphotobiol.2017.10.004

Cited by 40 publications

(30 citation statements)

References 23 publications

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“…It was also demonstrated in the same study that AlPcS mix had the most prominent effect when compared to a different PS, GePcS mix . In cervical cancer cells, PDT using a different metallised phthalocyanine, ZnPc has proven effective in inducing cell damage through activation of apoptosis, necrosis and autophagy [43, 44]. Our results demonstrated a similar effect of AlPcS mix in the eradication of cervical cancer HeLa cells.…”

Section: Discussionsupporting

confidence: 61%

Photodynamic therapy of cervical cancer by eradication of cervical cancer cells and cervical cancer stem cells

2019

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The heterogeneous nature of cancer puts cancer stem cells (CSCs) at the beating heart of the tumour. Because of their inherent characteristics of stemness, CSCs evade putative cancer therapies, resulting in treatment resistance or tumour recurrence after a seemingly successful treatment. To prevent treatment resistance and cancer recurrence, killing the beating heart of the tumour is of utmost importance. This study therefore, sought to determine the effect of Photodynamic Therapy (PDT) in eradicating cervical cancer and cervical CSCs. Cervical CSCs were isolated from a cervical adenocarcinoma cell line, HeLa cells, and grown in liquid medium incubated at 37° C, 5% CO 2 and 85% humidity. Increasing doses of AlPcS mix photosensitizer were administered to both the total cell population and the isolated CSCs, and irradiated using 673.2 nm diode laser. Post-irradiation cellular changes were observed using biochemical assays and microscopy to determine the response of both the total cell population and the CSCs. Results showed a dose-dependent response of both cell populations to treatment, by demonstration of significant morphologic changes, increased cytotoxicity, and decreased cell viability and proliferation. The study suggested that PDT using AlPcS mix is a very effective treatment method for the eradication of cervical cancer cells and cervical CSCs, in vitro .

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Section: Discussionsupporting

confidence: 61%

Photodynamic therapy of cervical cancer by eradication of cervical cancer cells and cervical cancer stem cells

2019

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“…Upon treatment with a testing drug, if the drug can rupture the cell membrane, cells release LDH to the culture medium and released LDH can be quantified (colorimetrically, fluorometrically) (Fotakis & Timbrell, ; Smith et al, ). A recent study conducted by Hodgkinson, Kruger, Mokwena, and Abrahamse () used the LDH assay to evaluate cell viability of cervical cancer cells (HeLa) against photodynamic therapy. A study carried out by Dasari et al () to assess anticancer effects of verteporfin in endometrial cancer cells (HEC‐1‐A and HEC‐1‐B), the LDH assay was used as a cell viability assay.…”

Section: Cell Viability/cytotoxicity and Antiproliferative Assaysmentioning

confidence: 99%

In vitro assays and techniques utilized in anticancer drug discovery

Ediriweera

Tennekoon

Samarakoon

2018

J of Applied Toxicology

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Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.

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“…Therapeutic benefits derived from such agents that utilize photodynamic action are known as photodynamic therapy (PDT). The beauty of PDT is its local action at targeted site without adverse systemic effects; it is therefore a popular and alternative option not only for dermatological disorders like vitiligo and psoriasis but also for squamous, basal, hepatocellular and cervical cell carcinoma [ 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

9-phenyl acridine photosensitizes A375 cells to UVA radiation

Hansda

Ghosh

2020

Heliyon

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Acridines are an important class of bioactive molecules having varied uses. Its derivative, 9-phenylacridine (ACPH) had been found to exhibit antitumor activity both in cell lines and in vivo model. Its DNA binding ability and absorbance in the ultraviolet range encouraged us to investigate its role as a photosensitizer with UVA radiation. We investigated the effects of ACPH prior to UVA exposure on in vitro DNA through photo-cleavage assay. Effect of such treatment was also studied in cultured A375 melanoma cells. Endpoints studied included morphological changes, evaluation of cellular viability, scratch assay, intracellular reactive oxygen species (ROS) production, DNA damage, lipid peroxidation, glutathione (GSH) level, autophagy, cell cycle progression, depletion of mitochondrial membrane potential (ΔΨmt), induction of apoptosis and Hoechst dye efflux assay. Our findings indicated that ACPH could sensitize damage to DNA induced by UVA both in vitro and in cells. It could also potentiate cell killing by UVA. It arrested cells in G 2 /M phase and induced apoptotic death through mitochondria mediated pathway. This sensitization was through enhancement of intracellular ROS. Our findings also indicated that the stem cells side population was reduced on such treatment. The findings are important as it indicates ACPH as a promising photosensitizer and indicates its possible role in photodynamic therapy.

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Cervical cancer cells (HeLa) response to photodynamic therapy using a zinc phthalocyanine photosensitizer

Cited by 40 publications

References 23 publications

Photodynamic therapy of cervical cancer by eradication of cervical cancer cells and cervical cancer stem cells

Photodynamic therapy of cervical cancer by eradication of cervical cancer cells and cervical cancer stem cells

In vitro assays and techniques utilized in anticancer drug discovery

9-phenyl acridine photosensitizes A375 cells to UVA radiation

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