Cervical Cancer Markers: Epigenetics and microRNAs

Laengsri, Vishuda; Kerdpin, Usanee; Plabplueng, Chotiros; Treeratanapiboon, Lertyot; Nuchnoi, Pornlada

doi:10.1093/labmed/lmx080

Cited by 89 publications

(77 citation statements)

References 133 publications

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“…MiRNAs may function either as oncomiRs or tumor suppressor miRNAs depending on their target genes (Xia et al, 2016). In particular, a wide range of miRNAs are dysregulated in cervical cancer and are involved in the modulation of various pathological and physiological phenomena, including cell proliferation, the cell cycle, apoptosis, metastasis, angiogenesis, and chemoresistance (Feng et al, 2017;Li et al, 2017;Laengsri et al, 2018). Therefore, miRNAs may be promising therapeutic targets for the management of cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor

et al. 2020

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Although the functions of long noncoding RNA (lncRNA) called FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) have been well studied in multiple human cancer types, its expression status and detailed roles in cervical cancer remain unknown and merit investigation. This study was aimed at assessing FOXD2-AS1 expression in cervical cancer and at determining its effects on the aggressive behavior of cervical cancer in vitro and in vivo. Expression of FOXD2-AS1 in cervical cancer tissues and cell lines was determined via reverse-transcription quantitative PCR. The effects of FOXD2-AS1 on cervical cancer cells were examined by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide (MTT) assay, flow-cytometric analysis, migration and invasion assays, and an in vivo tumorigenicity assay. FOXD2-AS1 was found to be significantly upregulated in cervical cancer tissues and cell lines. High FOXD2-AS1 expression was notably linked with the Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and depth of cervical invasion in patients with cervical cancer. Kaplan-Meier survival analysis revealed significantly shorter overall survival of patients when the tumor expression of FOXD2-AS1 was higher in comparison with those in patients with lower FOXD2-AS1 expression. In vitro functional assays revealed that downregulation of FOXD2-AS1 led to suppression of proliferation, migration, and invasiveness as well as to the induction of apoptosis of cervical cancer cells. In addition, FOXD2-AS1 silencing hindered tumor growth in vivo. Mechanism investigation revealed that FOXD2-AS1 functioned as a molecular sponge of microRNA-760 (miR-760). Furthermore, hepatoma-derived growth factor (HDGF) was validated as a direct target gene of miR-760 in cervical cancer cells. Moreover, an miR-760 knockdown reversed the effects of FOXD2-AS1 silencing on cervical cancer cells. FOXD2-AS1 possesses significant oncogenic activity in cervical cancer progression; this activity is mediated by sponging

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor

et al. 2020

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“…Indeed, the dysregulation of miRNAs has been reported to contribute to a number of processes associated with cancer pathophysiology, including cell proliferation, cell cycle, apoptosis, epithelial-mesenchymal transition, metastasis, angiogenesis and resistance to chemoand radiotherapy (32)(33)(34). Therefore, a thorough understanding of the mechanism underlying cervical cancer carcinogenesis and progression is crucial for early diagnosis and improving patient outcome (14). In particular, further exploration on the specific roles of dysregulated miRNAs in cervical cancer and their associated mechanism may provide an avenue for the development of effective therapeutic approaches.…”

Section: Discussionmentioning

confidence: 99%

“…To date, >2,500 miRNAs have been identified in the human genome (12), with ~60% of protein-coding genes reported to be targeted by miRNAs (13). Dysregulation of miRNA function has been documented in nearly all types of human malignant tumors such as cervical cancer (14)(15)(16). miRNAs may be involved in the modulation of cervical carcinogenesis and progression by acting as either oncogenes or tumor suppressors (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑889‑3p targets FGFR2 to inhibit cervical cancer cell viability and invasion

et al. 2019

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MicroRNAs (miRNAs) are frequently dysregulated in cervical cancer, and the aberrant regulation of miRNAs may be involved in the regulation of various cancer-associated biological processes. Therefore, further exploration of the specific roles of dysregulated miRNAs in cervical cancer and their associated mechanism may promote the development of effective therapeutic approaches. miRNA-889-3p (miR-889) serves crucial roles in esophageal squamous cell carcinoma and hepatocellular carcinoma. However, to the best of our knowledge, no studies concerning the relationship between miR-889 and cervical cancer were performed. The aims of this study were to measure miR-889 expression in cervical cancer and to examine the potential effects of miR-889 in cervical cancer development on a molecular level to provide potential clinical insight. The present study revealed that miR-889 was downregulated in cervical cancer tissues and cell lines. Reduced miR-889 expression was significantly associated with International Federation of Gynecology and Obstetrics cancer staging and with lymph node metastasis. In addition, miR-889 overexpression reduced cervical cancer cell viability and invasive ability. Using bioinformatics analysis, fibroblast growth factor receptor 2 (FGFR2) was predicted to be a potential target of miR-889, which was confirmed using luciferase reporter assay. Reverse transcription-quantitative PCR and western blot analysis results suggested that miR-889 overexpression decreased FGFR2 expression in cervical cancer cells at the mRNA and the protein level, respectively. Conversely, FGFR2 silencing using small interfering RNA imitated the tumor suppressive effects of miR-889 overexpression in cervical cancer cells, which was successfully reversed by plasmid-facilitated FGFR2 overexpression. These observations demonstrated that miR-889 may serve tumor suppressive roles in cervical cancer by directly targeting FGFR2, which indicated that this miRNA may be a promising therapeutic target for patients with cervical cancer.

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“…MicroRNAs (miRNAs) are small noncoding RNAs of 18‐22 nucleotides (nt) in length that are able to bind to the 3′‐UTR of specific mRNA targets to regulate their translation at the post‐transcriptional level . Several previous studies have demonstrated that some miRNAs are dysregulated in many cancers, especially in CC . For example, miR‐374b inhibited cell proliferation and induces apoptosis through the p38/ERK signaling pathway by binding to JAM‐2 in cervical cancer .…”

Section: Introductionmentioning

confidence: 99%

DANCR‐mediated microRNA‐665 regulates proliferation and metastasis of cervical cancer through the ERK/SMAD pathway

et al. 2019

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Emerging evidence has indicated that microRNAs (miRNAs) play an important role in cervical cancer (CC). However, the role of miRNA (miR)‐665 in cervical cancer remains unclear. The aim of the present study was to investigate the potential functions of miR‐665 in CC and to identify the underlying mechanisms of action. Herein, we show that miR‐665 was downregulated in CC tissues and cell lines, which is negatively correlated with tumor size, distant metastasis, advanced TNM stage and poor prognosis. Functionally, miR‐665 inhibited cell proliferation, migration and invasion and resistance of cisplatin for CC cells, as well as tumor growth. We validated that transforming growth factor beta receptor 1 (TGFBR1) was a direct target of miR‐665 and mediated the ERK/SMAD pathway. In addition, we identified miR‐665 as the competing endogenous RNA for long noncoding (lnc)‐DANCR. These observations suggested that lnc‐DANCR‐mediated miR‐665 downregulation regulates the malignant phenotype of CC cells by targeting TGFBR1 through the ERK/SMAD pathway, which may present a pathway for novel therapeutic stratagems for CC therapy.

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Cervical Cancer Markers: Epigenetics and microRNAs

Cited by 89 publications

References 133 publications

RETRACTED: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor

RETRACTED: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor

MicroRNA‑889‑3p targets FGFR2 to inhibit cervical cancer cell viability and invasion

DANCR‐mediated microRNA‐665 regulates proliferation and metastasis of cervical cancer through the ERK/SMAD pathway

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