Cervical Cancer

Greer, Benjamin E.; Koh, Wui-Jin; Abu‐Rustum, Nadeem R.; Apte, Sachin M.; Campos, Susana M.; Chan, John K.; Cho, Kathleen R.; Copeland, Larry J.; Crispens, Marta A.; duPont, Nefertiti C.; Eifel, Patricia J.; Gaffney, David K.; Huh, Warner K.; Kapp, Daniel S.; Lurain, John R.; Martin, Lainie P.; Morgan, Mark A.; Morgan, Robert J.; Mutch, David G.; Remmenga, Steven W.; Reynolds, R. Kevin; Small, William; Teng, Nelson N.H.; Valea, Fidel A.

doi:10.6004/jnccn.2010.0104

Cited by 64 publications

(43 citation statements)

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“…In addition, the complete genome sequence of HeLa has recently been determined, making it an ideal cellular model for cancer study [2, 35]. Cisplatin remains a backbone of therapy for early and late-stage cervical cancer [19], so it is of clinical interest that knocking out miR-21 enhances sensitivity of HeLa to cisplatin. Thus, our study further advances the idea that miR-21 may be explored as a safe adjuvant therapeutic target [10, 65].…”

Section: Discussionmentioning

confidence: 99%

Disruption of microRNA-21 by TALEN leads to diminished cell transformation and increased expression of cell–environment interaction genes

Chen

et al. 2015

Cancer Letters

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MicroRNA-21 is dysregulated in many cancers and fibrotic diseases. Since miR-21 suppresses several tumor suppressor and anti-apoptotic genes, it is considered a cancer therapeutic target. Antisense oligonucleotides are commonly used to inhibit a miRNA; however, blocking miRNA function via an antagomir is temporary, often only achieves a partial knock-down, and may be complicated by off-target effects. Here, we used transcription activator-like effector nucleases (TALENs) to disrupt miR-21 in cancerous cells. Individual deletion clones were screened and isolated without drug selection. Sequencing and quantitative RT-PCR identified clones with no miR-21 expression. The loss of miR-21 led to subtle but global increases of mRNAs containing miR-21 target sequences. Cells without miR-21 became more sensitive to cisplatin and less transformed in culture and in mouse xenografts. In addition to the increase of PDCD4 and PTEN protein, mRNAs for COL4A1, JAG1, SERPINB5/Maspin, SMAD7, and TGFBI – all are miR-21 targets and involved in TGFβ and fibrosis regulation – were significantly upregulated in miR-21 knockout cells. Gene ontology and pathway analysis suggested that cell-environment interactions involving extracellular matrix can be an important miR-21 pathogenic mechanism. The study also demonstrates the value of using TALEN-mediated microRNA gene disruption in human pathobiological studies.

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Section: Discussionmentioning

confidence: 99%

Disruption of microRNA-21 by TALEN leads to diminished cell transformation and increased expression of cell–environment interaction genes

Chen

et al. 2015

Cancer Letters

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“…In advanced stage disease (peritoneal carcinomatosis, pulmonary metastasis-stage IVB), chemotherapy alone is recommended for treatment. Additional RT in this setting is considered to carry risk for morbidity rather than to provide any survival benefit (Greer et al, 2010). Of the 18 study patients who had stage IVB disease, seven patients (47%) were treated by chemotherapy only.…”

Section: Discussionmentioning

confidence: 99%

Effect of Hydronephrosis on Survival in Advanced Stage Cervical Cancer

Goklu

Seçkin²,

Toğrul³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Hydronephrosis is frequently encountered in advanced stage cervical cancers, and may be associated with mortality. In the present study, we aimed to demonstrate the effect of hydronephrosis on survival in patients with inoperable advanced stage cervical cancer. Materials and Methods: The study data were acquired by retrospective analysis of the patient records belonging to 165 women with FIGO (International Federation of Gynecology and Obstetrics) stage-IIIB or more advanced cervical cancer, which were not surgical candidates. Parameters including patient age, pathological diagnosis, disease stage, pelvic sidewall extension, presence of hydronephrosis and administration of chemoradiation were analyzed. Further, the effects of these variables on survival were assessed. P values less than 0.05 were considered statistically significant. Results: The distribution of the study patients according to disease stage was as follows: 131 (79.4%) had stage-IIIB, 18 (10.9%) had stage-IVB and 16 (% 9.7) patients had stage-IVA disease. Hydronephrosis was not evident in 91 (55.2%) of these patients, whereas 41 (24.8%) had unilateral and 33 (20%) patients had bilateral hydronephrosis. When compared to mean survival in patients who did not have hydronephrosis, survival was significantly shortened in patients who had bilateral and unilateral hydronephrosis (p < 0.05). There was no significant survival difference between patients with unilateral and bilateral hydronephrosis (p>0.05). Although patient age, pathological type, pelvic involvement, and chemotherapy treatment rates were similar (p>0.05), radiotherapy requirement rate and disease stage were significantly different among the study groups (p<0.05). Conclusions: Hydronephrosis was found to be a significant predictor of poor survival in patients with advanced stage cervical cancer, irrespective of unilateral or bilateral involvement.While waiting for future studies with larger sample sizes, we believe that the FIGO stages in advanced cervical cancer could further be stratified into subgroups according to presence or absence of hydronephrosis.

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“…Adolescence is a crucial timeperiod in the natural history of HPV infection: in fact HPV infection is usually acquired during adolescence (up to 25 years old) and many of the risk factors for developing cervical cancer are typically found in adolescents. Cigarette smoking, a younger age at first intercourse, high number of sexual partners and other sexual transmitted infections are associated with an increase risk of invasive cervical cancer [78]. The incidence of infections among female adolescents aged 14-19 ranges from 18.2% to 24.5% [79,80] while it is >20% in young men [81].…”

Section: Human Papilloma Virus Vaccinesmentioning

confidence: 99%

Immunization in Adolescents: Past, Present and Future

Mameli¹

2011

TOVACJ

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Abstract:In the past, immunization programs worldwide mainly focused on the delivery of infant and early childhood vaccines. An increasing awareness of the importance of investing in adolescents' health has led to the introduction of new vaccines targeted specifically to adolescents over the last ten years: this has improved the adolescent's opportunities to protect from certain diseases for which they are at an increased risk. Safe and effective vaccines against human papilloma virus, Neisseria meningitides and Bordetella pertussis are recommended in many parts of the world; nevertheless, vaccination coverage in this age group is relatively low compared to coverage in infants. Barriers to adolescent immunization are believed to be complex and multifactorial but overcoming these barriers will be of primary importance for the future.

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Cervical Cancer

Cited by 64 publications

References 0 publications

Disruption of microRNA-21 by TALEN leads to diminished cell transformation and increased expression of cell–environment interaction genes

Disruption of microRNA-21 by TALEN leads to diminished cell transformation and increased expression of cell–environment interaction genes

Effect of Hydronephrosis on Survival in Advanced Stage Cervical Cancer

Immunization in Adolescents: Past, Present and Future

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