Objective:This study aims at cone-beam computed tomography (CBCT) evaluation of the ability of Gates Glidden (GG) drills, Protaper Sx, and LA Axxess burs to produce a straight line access (SLA) in mesiobuccal canals of mandibular first molars.Methodology:Forty-five freshly extracted mandibular teeth with a canal curvature of 10-20° were taken for the study and divided into three groups according to the instruments used for cervical preflaring: Group I (LA Axxess burs), Group II (GG drills), and Group III (Protaper Sx). Pre- and post-instrumentation CBCT images were evaluated for comparing the ability of GG drills, Protaper Sx and LA Axxess burs to produce an SLA in mesiobuccal canals of mandibular first molars.Results:There was no significant change (P = 0.06) in the angle in the preSLA images of LA Axxess Group I (12.37 ± 1.01), GG Group II (13.39 ± 1.74), and Protaper Sx Group III (13.90 ± 1.74). The mean decrease in the angle from preSLA to postSLA was significant for all the three groups (P = 0.0001). However, the mean change was highest in Group I (4.25 ± 1.14), followed by Group II (3.28 ± 1.22) and Group III (2.89 ± 1.53).Conclusion:LA Axxess burs were the most effective in reducing the coronal curvature and produced a straighter access to apical third compared to GG Drills and Protaper Sx.