Cervicovaginal Levels of Proinflammatory Cytokines Are Increased During Chlamydial Infection in Bacterial Vaginosis But Not in Lactobacilli-Dominated Flora

Marconi, Camila; Santos-Greatti, M. M. V.; Pàrada, Cristina Maria Garcia de Lima; Pontes, Anaglória; Pontes, Ana Gabriela; Giraldo, Paulo César; Donders, Gilbert; Silva, Márcia Guimarães da

doi:10.1097/lgt.0000000000000003

Cited by 18 publications

(14 citation statements)

References 31 publications

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“…This may constitute a possible mechanism by which inflammation contributes to HPV-mediated carcinogenesis. Furthermore, our results are in accordance with data from the literature that have already described the relation between C. trachomatis infection and high-grade cervical lesions (13)(14)(15)(16).…”

Section: Discussionsupporting

confidence: 83%

“…In conventional Pap smears, Matheus and colleagues (12) showed a high expression of MMP-9 in the presence of genital infections. Herein, we found a significant association between C. trachomatis and high-grade cervical lesions (CIN2/CIN3/SCC; OR, 3.7; 95% CI, 1.3-11.3), which has already been amply demonstrated in the literature (13)(14)(15)(16). In addition, we found that women with C. trachomatis have a 2.7 higher chance of presenting multiple infections than a single infection by HPV, although this result was not statistically significant in this sample size (OR, 2.7; 95% CI, 0.8-9.0).…”

Section: Discussionmentioning

confidence: 57%

“…Clinical studies have shown an association between high-grade cervical intraepithelial neoplasia (CIN) and Chlamydia trachomatis infection (13)(14)(15)(16). Considering that concomitant genital infections have been assumed to be risk factors for HPV persistence (14), we hypothesized that infection-induced cervical inflammation were capable of influencing the carcinogenic process driven by HPV.…”

Section: Introductionmentioning

confidence: 99%

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MMP-9/RECK Imbalance: A Mechanism Associated with High-Grade Cervical Lesions and Genital Infection by Human Papillomavirus and Chlamydia trachomatis

Discacciati

Gimenes

Pennacchi

et al. 2015

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Matrix metalloproteinases (MMP) are important enzymes in the tumor microenvironment associated with progression of cervical intraepithelial neoplasia (CIN) toward squamous cell carcinoma (SCC) of the cervix. However, the role of MMPs in the inflammatory process associated with Chlamydia trachomatis infection concomitant with the carcinogenic process driven by HPV has not yet been addressed. In the present study, we analyzed the state of the MMP-9-RECK axis in cervical carcinogenesis.Methods: The levels of MMP-9 and RECK expression were analyzed by immunocytochemistry in liquid-based cytology samples from 136 women with high-grade cervical lesions (CIN2/ CIN3) and cervical SCC diagnosed by LLETZ, and in 196 women without cervical neoplasia or CIN1. Real-time qPCR was performed to analyze expression of MMP-9 and RECK in 15 cervical samples. The presence of HPV-DNA and other genital pathogens was evaluated by PCR.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

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MMP-9/RECK Imbalance: A Mechanism Associated with High-Grade Cervical Lesions and Genital Infection by Human Papillomavirus and Chlamydia trachomatis

Discacciati

Gimenes

Pennacchi

et al. 2015

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Mitchell et al reported a relationship between BV and decreased HBD2 expression but no relation to IL-6 (26). On the other hand in Chlamydia trachomatis infection, IL-6 is reportedly increased with BV patients but not with Lactobacillus dominant flora (27). Our results suggested that IL-6 might not be strongly involved in the pathogenesis of BV and Candidiasis in some vaginal environments ( Table 3 and…”

Section: Discussionmentioning

confidence: 60%

Relationship Between Human β-Defensin 2 and the Vaginal Environment

et al. 2020

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As one of the main antimicrobial peptides, human β-defensin 2 (HBD2) plays multiple roles in the lower genital tract. Based on the Nugent score as a diagnostic criterion for bacterial vaginosis, we sought to clarify the correlations among the Nugent score and Interleukin-6 (IL-6) and HBD2 levels in vaginal secretions in association with various types of infection. Ninety-eight women were recruited for this study. Levels of HBD2 and IL-6 in the vaginal wash were measured by enzyme-linked immunosorbent assays. According to the Nugent method, the number of Lactobacillus morphotypes per field of view was well correlated with the HBD2 level. The amount of HBD2 was also well correlated with the presence of Candida spp.(p<0.01). In vitro experiments revealed that the expression of HBD2 from the human vaginal epithelial cell line, VK2/E6E7, was induced by the addition of heat-killed Candida albicans (HKCA). The addition of HKCA induced the expression of Dectin-1 mRNA, and a luciferase assay for NF-kB responsive elements showed that HKCA activated NF-kB signaling. These results suggested that Candida albicans would be induced the activation of Dectin-1 and NF-kB signaling, resulting in HBD2 expression. In conclusion, the expression of HBD2 was positively correlated with the presence of Lactobacillus and Candida spp..

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“…При воспалительных заболеваниях вульвы и влагалища также отмечается повышение синтеза ФНО-альфа и ИЛ-1, в т. ч. и у беременных пациенток [9][10][11]. Повы шенный цитокиновый фон, высокая активность G. vagi nalis на фоне дополнительной супрессии нормальной микрофлоры влагалища антисептиками могут провоцировать быструю гибель нормальных клеток слизистой в зоне воспаления в результате процесса пироптоза [12,13].…”

Section: патогенетическое обоснование применения бензидамина при острunclassified

Mechanisms of benzydamine action against local inflammatory process

2019

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Local inflammatory diseases caused by various infections are one of the most common pathologies in medical practice. For example, tonsillopharyngitis. This disease is extremely frequent for a physician’s practice. There is a fair amount of drugs, which supposed to be helpful against tonsillopharyngitis, but different drugs are also not the same in their pharmacological effects. In gynecological practice, frequent diseases are specific and non-specific vulvovaginitis, which have inflammatory and infectious components. For administrating local pharmacotherapy in gynecology against inflammation, a large number of drugs with different mechanisms of action are proposed. In this study, we focused on key pathological mechanisms associated with acute and chronic inflammation, for which these drugs should be exposed by their pharmacokinetic and pharmacodynamic properties. The best combination of these properties is available for benzydamine hydrochloride. Benzydamine hydrochloride has a wide antimicrobic activity against bacteria and Candida spices both albicans and non-albicans strains and allows to influence on etiologic cause of the disease. Also benzydamine hydrochloride associated with «cytokine» mechanism of anti-inflammatory action, which means that he does not affect COX enzymes and it allows to avoid gastrotoxic adverse events. Also, in this work showed and discussed aspects of the interaction of benzydamine with local immune system and justification of useful usage the benzydamine for local therapy of acute and chronic inflammatory processes caused by various infections.

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Cervicovaginal Levels of Proinflammatory Cytokines Are Increased During Chlamydial Infection in Bacterial Vaginosis But Not in Lactobacilli-Dominated Flora

Abstract: Detection of endocervical CT is associated with increased cervicovaginal IL-1β, IL-6, and IL-8 levels in women with concomitant BV but not in those with normal flora.

Cited by 18 publications

References 31 publications

MMP-9/RECK Imbalance: A Mechanism Associated with High-Grade Cervical Lesions and Genital Infection by Human Papillomavirus and Chlamydia trachomatis

MMP-9/RECK Imbalance: A Mechanism Associated with High-Grade Cervical Lesions and Genital Infection by Human Papillomavirus and Chlamydia trachomatis

Relationship Between Human β-Defensin 2 and the Vaginal Environment

Mechanisms of benzydamine action against local inflammatory process

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