Cervids as Babesiae Hosts, Slovenia

Duh, Darja; Petrovec, Miroslav; Bidovec, A.; Avšič–Županc, Tatjana

doi:10.3201/eid1107.040724

Cited by 89 publications

(105 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The parasites were identified by nested PCR, and sequence analysis of the 18SrRNA showed 99.7% of identity of the roe deer Babesia EU1 with the human strain EU1. The Babesia divergens detected in cervids was 99.6% identical to bovine B. divergens, which suggests that the roe deer and red deer may serve as a reservoir for B. divergens (Duh et al, 2005). Therefore, our findings support that deer may be a reservoir of bovine Babesia species.…”

supporting

confidence: 66%

Immunologic and Molecular Identification of Babesia bovis and Babesia bigemina in Free-Ranging White-Tailed Deer in Northern Mexico

Cantu

Ortega-S.

Mosqueda

et al. 2007

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:The suitability of white-tailed deer (Odocoileus virginianus) as hosts for the cattle ticks Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus, has been well documented. These ticks have a wide host range, and both transmit Babesia bovis and Babesia bigemina, the agents responsible for bovine babesiosis. Although this disease and its vectors have been eradicated from the United States and some states in northern Mexico, it still is a problem in other Mexican states. It is not known if wild cervids like white-tailed deer can act as reservoirs for bovine babesiosis. The purpose of this study was to determine if B. bovis and B. bigemina or antibodies against them occur in white-tailed deer in the states of Nuevo Leon and Tamaulipas, Mexico. Twenty blood samples from white-tailed deer from two ranches were collected and tested with a nested polymerase chain reaction (nested PCR) and indirect immunofluorescence antibody test (IFAT) for B. bovis and B. bigemina. Eleven samples were positive for B. bigemina and four for B. bovis by nested PCR; amplicon sequences were identical to those reported in GenBank for B. bovis (Rap 1) and B. bigemina. Results of the IFA test showed the presence of specific antibodies in serum samples. This is the first report of the presence of B. bovis and B. bigemina in white-tailed deer using these techniques and underscores the importance of cervids as possible reservoirs for bovine babesiosis.

show abstract

supporting

confidence: 66%

Immunologic and Molecular Identification of Babesia bovis and Babesia bigemina in Free-Ranging White-Tailed Deer in Northern Mexico

Cantu

Ortega-S.

Mosqueda

et al. 2007

Journal of Wildlife Diseases

View full text Add to dashboard Cite

show abstract

“…In contrast, Babesia sp. EU1 which has repeatedly been identified in game or in ticks in Europe (Casati et al, 2006;Duh et al, 2005a;Duh et al, 2005b;Hilpertshauser et al, 2006 and this work) and which also has been reported in three splenectomized human patients (Haselbarth et al, 2007;Herwaldt et al, 2003), differs by 3% in this gene sequence. The analyses of part of the 28S rRNA gene and of the more variable locus, the rDNA ITS, revealed again a high similarity, but the isolates from cattle could clearly be differentiated from those of free-ranging ruminants (Fig.…”

Section: Discussionmentioning

confidence: 85%

“…EU1, and first studies revealed prevalences of up to 30% (Bonnet et al, 2007;Duh et al, 2005b;Hoby et al, 2007b). Interestingly, the parasites seem to have a low virulence in roe deer, and it may be speculated that roe deer acts as a reservoir for the parasite affecting chamois.…”

Section: Discussionmentioning

confidence: 99%

“…In other European countries, Babesia sp. were reported in free-ranging roe deer (Capreolus c. capreolus), red deer (Cervus elaphus) (Duh et al, 2005b) and a farmed reindeer (Rangifer t. tarandus) (Langton et al, 2003) which were genetically most similar to B. divergens, the known pathogen of cattle. The authors suggested that wild ruminants might act as possible reservoirs for B. divergens.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Schmid

Deplazes

Hoby

et al. 2008

Veterinary Parasitology

View full text Add to dashboard Cite

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation Schmid, N Schmid, N. Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation. 2008 Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation AbstractIn 2005 and 2006, three adult female chamois (Rupicapra r. rupicapra) were found dead with signs of acute babesial infection in the eastern Swiss Alps. PCR on DNA extracted from blood or spleen of the carcasses revealed sequence identity of the amplified part of the 18S rRNA gene with GenBank entries attributed to Babesia divergens of cattle origin or B. capreoli of wild ruminant origin which have never been described before in this region. Examination of 424 blood samples from 314 head of cattle from this area by IFAT, microscopy and PCR provided no evidence for babesial infection. Six of 887 ticks collected from cattle were PCR-positive, and sequencing revealed Babesia sp. genotype EU1 in five and B. divergens/B. capreoli in one of them. A Babesia isolate of chamois, two isolates of roe deer from the same region and one isolate of a roe deer from the north-western Swiss Alps were genetically compared with two Swiss B. divergens isolates of cattle origin by analysing the genomic rDNA locus. Whereas the near full length sequences of the 18S rRNA gene were virtually identical among all six isolates (>99.4% identity), distinct differences between the two isolates from cattle on the one hand and the four isolates from free-ranging ruminants on the other hand were observed in the sequences of the internal transcribed spacers 1 and 2 (ITS1, ITS2) and part of the 28S rRNA gene. These results indicate that, albeit genetically very closely related, these babesial organisms from cattle and from free-ranging ruminants indeed are distinguishable organisms with different host specificities, and they support the use of the discrete species name B. capreoli for the B. divergens-like organisms from chamois and roe deer. were virtually identical among all six isolates (>99.4% identity), distinct differences between the two isolates from cattle on the one hand and the four isolates from freeranging ruminants on the other hand were observed in the sequences of the internal transcribed spacers 1 and 2 (ITS1, ITS2) and part of the 28S rRNA gene. These results indicate that, albeit genetically very closely related, these babesial organisms from cattle and from free-ranging ruminants indeed are distinguishable organisms with different host specificities, and they support the use of the discrete species name B. capreoli for the B. ...

show abstract

“…B. divergens has also been found in humans and wild ruminants, roe (Capreolus capreolus), and red deer (Cervus elaphus), Duh et al 2005;Zintl et al 2011), although the designation of red deer as a host is still under discussion. B. divergens is endemic in the southern part of Belgium (Losson and Lefevre 1989) but is considered absent from other parts of the country (Saegerman et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium

Lempereur

Lebrun²,

Cuvelier³

et al. 2011

Parasitol Res

View full text Add to dashboard Cite

Anaplasmosis and babesiosis are major tick-borne diseases with a high economic impact but are also a public health concern. Blood samples collected in the spring, summer, and autumn of 2010 from 65 cows in seven different farms in Belgium were monitored with an indirect immunofluorescence antibody test to assess seroprevalence against these pathogens. Seroprevalences to Babesia spp. were measured as 10.7%, 20%, and 12.3% in spring, summer, and autumn, respectively, whereas seroprevalences to Anaplasma phagocytophilum were 30.8%, 77%, and 56.9%, respectively. A total of 805 Ixodes ricinus ticks were collected at the same time from both cattle (feeding ticks) and grazed pastures (questing ticks). The infection level of ticks, assessed by PCR assay, for Babesia spp. DNA was 14.6% and 7.9% in feeding and questing ticks, respectively, whereas 21.7% and 3% of feeding and questing ticks were found be positive for A. phagocytophilum cDNA. Fifty-five PCR-positive samples were identified by sequencing as Babesia sp. EU1, of which five from feeding ticks were positive for both A. phagocytophilum and Babesia sp. EU1. The high density of wild cervids in the study area could explain these observations, as deer are considered to be the main hosts for adults of I. ricinus. However, the absence of Babesia divergens both in feeding and questing ticks is surprising, as the study area is known to be endemic for cattle babesiosis. Increasing cervid populations and comorbidity could play an import role in the epidemiology of these tickborne diseases.

show abstract

Cervids as Babesiae Hosts, Slovenia

Cited by 89 publications

References 14 publications

Immunologic and Molecular Identification of Babesia bovis and Babesia bigemina in Free-Ranging White-Tailed Deer in Northern Mexico

Immunologic and Molecular Identification of Babesia bovis and Babesia bigemina in Free-Ranging White-Tailed Deer in Northern Mexico

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Longitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium

Contact Info

Product

Resources

About