Cesiribacter andamanensis gen. nov., sp. nov., isolated from a soil sample from a mud volcano

Abstract

A novel Gram-staining-negative, rod-shaped, non-motile bacterium, strain AMV16T, was isolated from a soil sample collected from a mud volcano located in the Andaman Islands, India. The cell suspension was pale orange. Cells of strain AMV16T were positive for catalase, oxidase, lipase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase and urease. The fatty acids present were anteiso-C11 : 0 (5.4 %), anteiso-C12 : 0 (4.1 %), C12 : 0 (7.0 %), iso-C15 : 0 (14.4 %), anteiso-C15 : 0 (3.4 %), anteiso-C16 : 0 (3.0 %), C16 : 0 (2.6 %), anteiso-C17 : 0 (3.7 %), iso-C19 : 0 (9.7 %), C13 : 1 (13.8 %), iso-C15 : 1 G (15.9 %), iso-C16 : 1 G (11.1 %) and summed feature 5 (anteiso-C18 : 0 and/or C18 : 2ω6,9c; 5.9 %). Strain AMV16T contained MK-4 as the major respiratory quinone and diphosphatidylglycerol and phosphatidylethanolamine made up the phospholipids. The G+C content of DNA of strain AMV16T was 50.9 mol%. blast sequence similarity searches based on the 16S rRNA gene sequence indicated that species of the genus Marivirga were the nearest phylogenetic neighbours, with pairwise sequence similarity ranging from 89.9 to 90.0 %. Phylogenetic analyses indicated that strain AMV16T clustered with the type strains of Marivirga tractuosa and Marivirga sericea at a phylogenetic distance of 14.6 % (85.4 % similarity), distinct from clades representing other genera of the family ‘Flammeovirgaceae’. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain AMV16T is proposed as a representative of a new genus and novel species, Cesiribacter andamanensis gen. nov., sp. nov. The type strain of Cesiribacter andamanensis is AMV16T ( = DSM 22818T = CCUG 58431T).

Extracellular Polysaccharide Production by a Novel Osmotolerant Marine Strain of Alteromonas macleodii and Its Application towards Biomineralization of Silver

“…DNA isolation, 16S rRNA gene amplification and sequencing were done as described previously [16]. The 16S rRNA gene sequence of the isolate was subjected to BLAST sequence similarity search [17] and EzTaxon [18] to identify the nearest related taxa.…”

Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater

“…Chemotaxonomic analysis and DNA G?C content Chemotaxonomic characteristics such as fatty acids, polar lipids and quinones were determined by previously described methods (Srinivas et al 2011a, b). DNA G?C content was performed by the membrane filter method as described previously (Srinivas et al 2011a).…”

“…DNA G?C content was performed by the membrane filter method as described previously (Srinivas et al 2011a). …”

“…Growth, biochemical and pigment characteristics were determined by previously described methods (Anil Kumar et al 2010;Srinivas et al 2011a). Biochemical and enzymatic characterization of the strain AK5 T was also performed using Vitek 2 GN kits (bioMérieux) with incubation at 30°C, according to the manufacturer's protocol, except that sterile 2.0% (w/v) NaCl was used to prepare the inoculum.…”

“…16S rRNA gene sequence determination and phylogenetic analysis DNA isolation, 16S rRNA gene amplification and sequencing were done as described previously (Pandey et al 2002;Srinivas et al 2011a). The 16S rRNA gene sequence of the isolate was subjected to BLAST sequence similarity search (Altschul et al 1990) and EzTaxon (Chun et al 2007) to identify the nearest related taxa.…”