Cetacean Intracytoplasmic Eosinophilic Globules: A Cytomorphological, Histological, Histochemical, Immunohistochemical, and Proteomic Characterization

Fernandez, A.; Câmara, Nakita; Sierra, Eva; Hernández, Manuel Antonio Arbelo; Quirós, Yara Bernaldo de; Jepson, Paul D.; Deaville, Rob; Díaz‐Delgado, Josué; Suárez-Santana, Cristian; Castro-Alonso, Ayoze; Hernández, Julia N.; Godinho, Ana

doi:10.3390/ani13132130

Cited by 3 publications

(2 citation statements)

References 95 publications

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“…Immunohistochemistry against myoglobin and fibrinogen was performed in cases 6, 7 and 9 using previously published methodology [19][20][21]. Briefly, 3 µm thick sections were deparaffined, rehydrated, and labelled with polyclonal anti-myoglobin and anti-fibrinogen anti-bodies (Abcam, Cambridge, United Kingdom), secondary anti-body consisted in polyclonal swine anti-rabbit immunoglobulin (Dako, Glostrup, Denmark) and were visualized using the VECTASTAIN Elite ABC-Peroxidase kit (PK-6100, Vector Laboratories, Peterborough, UK).…”

Section: Methodsmentioning

confidence: 99%

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Blunt Force Trauma in the Canarian Houbara Bustard (<em>Chlamyd-Otis Undulata Fuertaventurae</em>) Produced by Collision with Over-Head Lines

Suárez-Santana,

Marrero-Ponce,

Navarro-Sarmiento

et al. 2024

Preprint

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The mortality of birds resulting from collisions and electrocutions with overhead lines, such as power lines, and phone line among others, has been implicated in the decline of various avian species globally. Specifically, overhead line collisions pose a significant threat to the conserva-tion of the Canarian houbara bustard (Chlamydotis undulata fuertaventurae), an endangered sub-species endemic to the Canary Islands. This study centres on the postmortem findings of Canarian houbara bustards that have collided with overhead lines, providing insights into the post-collision outcomes for these birds. A com-plete standardized necropsy of nine Canarian houbara bustards revealed that polytrauma was the cause of death in all cases. The most notable gross lesions associated with trauma included bone fractures, soft tissue lacerations, haemorrhages, luxations, and hemocoelom, with certain body regions being more frequently affected. Histopathology, immunohistochemistry, and en-tomology analysis confirmed that numerous birds survived the initial trauma, exhibiting varia-ble survival intervals before succumbing to their injuries. We concluded that when a houbara bustard collide with an overhead line it frequently survives the initial trauma. The histopathology, immunohistochemistry, or entomologic analysis may be helpful to approximate the timing interval between trauma and death.

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Section: Methodsmentioning

confidence: 99%

“…Positive control for myoglobin included the skeletal muscle of each case (internal control) and skeletal muscle from a dolphin with rhabdomyolysis (external control) [20]. Positive control of fibrinogen was the liver of a live-stranded dolphin with extensive hepatocyte vacuolar degeneration [19,21].…”

Section: Methodsmentioning

confidence: 99%

Blunt Force Trauma in the Canarian Houbara Bustard (<em>Chlamyd-Otis Undulata Fuertaventurae</em>) Produced by Collision with Over-Head Lines

Suárez-Santana,

Marrero-Ponce,

Navarro-Sarmiento

et al. 2024

Preprint

View full text Add to dashboard Cite

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Food Safety: Pathological and Biochemical Responses of Nile Tilapia (Oreochromis niloticus) to Parasitological Infestation and Heavy Metals Pollution in Aquaculture System, Jeddah, Saudi Arabia

Alsulami,

Baowidan,

Aljarari

et al. 2024

Animals

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Objective: The study aims to assess the overall safety of cultured tilapias in Jeddah City, Saudi Arabia by assessing the impact of infection and anthropogenic pollution on farmed tilapias based on fish sex, body weight, length, and heavy metals contamination. Materials and methods: A total of 111 fish were collected from an aquaculture farm in Hada Al-Sham, Jeddah, Saudi Arabia. Physicochemical parameters of water from the culture system were evaluated. Both ecto- and endoparasites were checked. Haematological, biochemical and histopathological investigations were evaluated. In addition, heavy metals, namely, cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), and zinc (Zn) were evaluated in different fish tissues and water samples from the aquaculture system. Results: The study revealed stressed aquaculture system. Tilapias were infested by both ectoparasites including Trichodina, Icthyophthirius multifiliis, Dactylogrus, and Cichlidogyrus, and endoparasites as Icthyophonus hoferi, the nematode Capillaria and coccidian protozoa. The study showed that male tilapias had greater infestation rates than females and longer and heavier male fish tended to be more susceptible to Dactylogyrus infection. Infected fish showed altered biochemical markers with subsequent increases in inflammatory and oxidative stress markers. The post-mortem lesion in the skin, gill lamellae, intestine, spleen, and liver showed significant pathological remarks. All investigated fish tissues revealed higher rates of heavy metals bioaccumulation compared to the surrounding waters. On the other hand, infected Nile tilapia tissues showed higher rate of metals accumulation compared to non-infected ones. Metals accumulated at a higher rate in the liver followed by kidney, intestine, gills, and muscles, respectively. Conclusions: This study is recognized as the first to address the food safety of farmed tilapias in Jeddah, Saudi Arabia. The results emphasized a significant relation between parasites and heavy metal in disrupting fish defense systems and harming fish’s physiological homeostasis and the histological state of tissues. The parasitized and polluted farmed fish pose health risk to humans due to possible zoonosis from parasitic infections and its subsequent bacterial infections with long-term exposure to toxic chemicals. Addressing the need for a combination of improved aquaculture practices, and stringent regulatory oversight.

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Blunt Force Trauma in the Canarian Houbara Bustard (Chlamydotis undulata fuertaventurae) Produced by Collision with Overhead Lines

Suárez-Santana,

Marrero-Ponce,

Navarro-Sarmiento

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

The mortality of birds resulting from collisions and electrocutions with overhead lines, such as power lines and phone lines, among others, has been implicated in the decline of various avian species globally. Specifically, overhead line collisions pose a significant threat to the conservation of the Canarian houbara bustard (Chlamydotis undulata fuertaventurae), an endangered subspecies endemic to the Canary Islands. This study centers on the postmortem findings of Canarian houbara bustards that have collided with overhead lines, providing insights into the post-collision outcomes for these birds. A complete standardized necropsy of nine Canarian houbara bustards revealed that trauma was the cause of death in all cases. The most notable gross lesions associated with trauma included bone fractures, soft tissue lacerations, hemorrhages, luxations, and hemocoelom. The inguinal area, chest, and wings were the body regions more frequently affected. A histopathology, immunohistochemistry, and entomology analysis confirmed that numerous birds survived the initial trauma. We concluded that when a houbara bustard collides with an overhead line, it frequently survives the initial trauma, with a survival time ranging from minutes to hours. The histopathology, immunohistochemistry, or entomologic analysis may be helpful to approximate the timing interval between trauma and death.

show abstract

Cetacean Intracytoplasmic Eosinophilic Globules: A Cytomorphological, Histological, Histochemical, Immunohistochemical, and Proteomic Characterization

Cited by 3 publications

References 95 publications

Blunt Force Trauma in the Canarian Houbara Bustard (<em>Chlamyd-Otis Undulata Fuertaventurae</em>) Produced by Collision with Over-Head Lines

Blunt Force Trauma in the Canarian Houbara Bustard (<em>Chlamyd-Otis Undulata Fuertaventurae</em>) Produced by Collision with Over-Head Lines

Food Safety: Pathological and Biochemical Responses of Nile Tilapia (Oreochromis niloticus) to Parasitological Infestation and Heavy Metals Pollution in Aquaculture System, Jeddah, Saudi Arabia

Blunt Force Trauma in the Canarian Houbara Bustard (Chlamydotis undulata fuertaventurae) Produced by Collision with Overhead Lines

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