Cetylpyridinium chloride functionalized silica‐coated magnetite microspheres for the solid‐phase extraction and pre‐concentration of ochratoxin A from environmental water samples with high‐performance liquid chromatographic analysis

Hu, Meihua; Huang, Pengcheng; Suo, Lili; Wu, Fang

doi:10.1002/jssc.201601464

Cited by 6 publications

(3 citation statements)

References 31 publications

(43 reference statements)

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“…We are also exploring the use of a preconcentration step in conjunction with this system to achieve higher effective concentrations of the toxicant analytes. 77 − 79 …”

Section: Resultsmentioning

confidence: 99%

“…Current efforts in our laboratory are focused on lowering the detection limits for cases where the detection limits are above the literature-reported levels of concern using other fluorophores and/or cyclodextrin hosts. We are also exploring the use of a preconcentration step in conjunction with this system to achieve higher effective concentrations of the toxicant analytes. − …”

Section: Resultsmentioning

confidence: 99%

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Detection of Organochlorine Pesticides in Contaminated Marine Environments via Cyclodextrin-Promoted Fluorescence Modulation

et al. 2017

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The development of practical and robust detection methods for pesticides is an important research objective owing to the known toxicity, carcinogenicity, and environmental persistence of these compounds. Pesticides have been found in bodies of water that are located near areas where pesticides are commonly used and easily spread to beaches, lakes, and rivers; affect the species living in those waterways; and harm humans who come into contact with or eat fish from such water. Reported herein is the rapid, sensitive, and selective detection of four organochlorine pesticides in a variety of water sources across the state of Rhode Island using cyclodextrin-promoted fluorescence detection. This method relies on the ability of cyclodextrin to promote analyte-specific fluorescence modulation of a high quantum yield fluorophore when a pesticide is in close proximity, combined with subsequent array-based statistical analyses of the measurable changes in the emission signals. This system operates with high sensitivity (low micromolar detection limits), selectivity (100% differentiation between structurally similar analytes), and general applicability (for different water samples with varying salinity and pH as well as for different water temperatures).

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“…We are also exploring the use of a preconcentration step in conjunction with this system to achieve higher effective concentrations of the toxicant analytes. 77 − 79 …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Detection of Organochlorine Pesticides in Contaminated Marine Environments via Cyclodextrin-Promoted Fluorescence Modulation

et al. 2017

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“…OTA has become a very common contaminant in food and the ecosystem. There is evidence of the presence of OTA in water sources (Hu et al, 2017;Mata et al, 2015) and sea animals (Sun et al, 2015). The highest incidence of detection, however, occurs in food.…”

Section: Introductionmentioning

confidence: 99%

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

Valadas

Sachett

Marcon

et al. 2021

Preprint

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Ochratoxin A (OTA) is a mycotoxin produced by species of filamentous fungi widely found as a contaminant in food and with high toxic potential. Studies have shown that this toxin cause kidney and liver damage, however, data on the effects of exposure to OTA on the central nervous system are still scarce. Zebrafish (Danio rerio) is a teleost often used in translational research due to its physiological, genetic, and behavioral homology with mammals, in addition to being useful as an environmental bioindicator. Thus, this study aimed to investigate the effects of exposure to OTA on behavioral and neurochemical parameters in adult zebrafish. The animals were treated with different doses of OTA (1.38, 2.77, and 5.53 mg/kg) and submitted to behavioral evaluations in the open tank and social interaction tests. Subsequently, they were euthanized, and the brains were used to assess markers associated with oxidative status. In the open tank test OTA induced changes in distance, absolute turn angle, mean speed, and time-freezing. However, no significant effects were observed in the social interaction test. Moreover, OTA also induced alterations in neurochemical parameters with changes in non-protein thiols (NPSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). This study showed that OTA can affect neurobiological aspects in zebrafish even at low doses.

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Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC

Huang

Suo

et al. 2018

Microchim Acta

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A polydopamine-based molecularly imprinted polymer was deposited on the surface of magnetite (ferroferric oxide) nanoparticles (FeO@PDA MIPs) and is shown to be an efficient and fairly specific sorbent for the extraction of various ochratoxins. The MIPs were characterized by IR spectroscopy and transmission electron microscopy. The adsorption capacities, evaluated through the langmuir adsorption isotherm model, are 1.8, 0.23 and 0.17 mg·g for ochratoxin A, ochratoxin B and ochratoxin C, respectively. Parameters such as the amount of magnetic MIPs, pH value, time for ultrasonication, elution solvent and volume were optimized. Following desorption from the MIP with acetonitrile, the ochratoxins were quantified by HPLC with fluorometric detection. Under optimal experimental conditions, the calibration plots are linear in the range of 0.01-1.0 ng·mL of OTA, 0.02-2.0 ng·mL of OTB, and 0.002-0.2 ng·mL of OTC. The LODs are between 1.8 and 18 pg·mL, and the recoveries from spiked samples are 71.0% - 88.5%, with RSDs of 2.3-3.8% in case of rice and wine samples. The MIPs can be re-used for at least 7 times. Graphical abstract Schematic of the preparation of a magnetic molecularly imprinted polymer based on self-polymerization of dopamine in weakly alkaline solution. Ochratoxins are recognized owing to homologous cavities in the MIPs, and quantified by HPLC after desorption with acetonitrile.

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Cetylpyridinium chloride functionalized silica‐coated magnetite microspheres for the solid‐phase extraction and pre‐concentration of ochratoxin A from environmental water samples with high‐performance liquid chromatographic analysis

Cited by 6 publications

References 31 publications

Detection of Organochlorine Pesticides in Contaminated Marine Environments via Cyclodextrin-Promoted Fluorescence Modulation

Detection of Organochlorine Pesticides in Contaminated Marine Environments via Cyclodextrin-Promoted Fluorescence Modulation

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC

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