cfa-miR-143 Promotes Apoptosis via the p53 Pathway in Canine Influenza Virus H3N2-Infected Cells

Abstract

MicroRNAs regulate multiple aspects of the host response to viral infection. This study verified that the expression of cfa-miR-143 was upregulated in vivo and in vitro by canine influenza virus (CIV) H3N2 infection. To understand the role of cfa-miR-143 in CIV-infected cells, the target gene of cfa-miR-143 was identified and assessed for correlations with proteins involved in the apoptosis pathway. A dual luciferase reporter assay showed that cfa-miR-143 targets insulin-like growth factor binding protein 5 (Igfbp5). Furthermore, a miRNA agomir and antagomir of cfa-miR-143 caused the downregulation and upregulation of Igfbp5, respectively, in CIV-infected madin-darby canine kidney (MDCK) cells. This study demonstrated that cfa-miR-143 stimulated p53 and caspase3 activation and induced apoptosis via the p53 pathway in CIV H3N2-infected cells. In conclusion, CIV H3N2 induced the upregulation of cfa-miR-143, which contributes to apoptosis via indirectly activating the p53-caspase3 pathway.

Effects of AntagomiRs on Different Lung Diseases in Human, Cellular, and Animal Models

“…Concerning the miRNAs studied, as above, a slight prevalence (three studies: Bhattacharyya et al [29] on cystic fibrosis, Podsiad et al [31] on pneumonia, Zhou et al [86] on systemic lupus erythematosus) included miR-155, with the positive results about the use of the respective antagomiR seen in vivo that were confirmed in vitro. miR-143 was successfully tailored in two studies (Tamgue et al [89] on tuberculosis, Zhou et al, [88] on influenza) confirming the positive retrievals already seen in vivo.…”

“…Sixteen studies on animals were found for this macro-category, with the condition more largely studied being influenza (four studies). All but two of these studies were carried out on mice or rats, whereas Asquith et al [85], studied chronic ethanol consumption in non-human primates, and Zhou et al [88] assessed the effect of miRNAs and antagomiRs in influenza in one-month-old beagles.…”

“…Three studies [31,72,85] on chronic ethanol consumption, pneumonia, and systemic lupus erythematosus, investigated the role of miR-155, two [88,89] respectively on tuberculosis and influenza, of miR-143 and the others showed complete heterogeneity in the miRNAs studied.…”

“…Nineteen studies were included in this category, most of which were already mentioned in the two previous (humans and animals) groups, as performed both in vivo and in vitro. Three of them were performed on influenza [86,88,90] and three others on lung fibrosis [26,82,84]. Other conditions were more rarely studied.…”

High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses

“…Furthermore, antiviral proteins produced from human lung epithelial cells and dendritic cells were controlled by several influenza A virus-mediated microRNAs [33,34,35]. In addition to human cells, few studies demonstrated in vivo and in vitro microRNA expression in dogs infected with canine influenza A viruses [36,37]. Nonetheless, canine microRNAs’ response to seasonal human influenza virus infection has not been reported yet.…”