The jet impact-negative pressure reactor (JI-NPR) is a novel wastewater treatment technology developed for the efficient removal of high-concentration ammonia nitrogen. However, the complex and transient nature of the flow behavior within the JI-NPR poses significant challenges for understanding the underlying fluid dynamics. In this work, a comprehensive signal-processing framework was developed to elucidate the flow characteristics inside the JI-NPR. First, a flow signal acquisition platform was established to capture the negative pressure signals during the treatment process. The empirical mode decomposition (EMD) technique was then employed to decompose the turbulent flow signals into a series of intrinsic mode functions (IMFs), representing multiscale turbulent eddy characteristics. To mitigate the effects of local noise and abrupt changes, various curve fitting methods, including cubic spline interpolation, piecewise cubic Hermite interpolating polynomial, and Makima interpolation, were utilized to smooth the IMF signals. The Hilbert transform was subsequently applied to extract the instantaneous frequency features of the smoothed IMFs, enabling more accurate quantification of the nonstationary and nonlinear flow behavior. The results revealed that the low-frequency IMFs were associated with the interactions between the wastewater jet and negative pressure, while the highfrequency IMFs reflected the internal dynamic evolution of the fluid. Furthermore, the multiple regression analysis approach was adopted to quantify the relationship between the IMF feature parameters and the critical performance metric of the denitrification efficiency. The decision tree regression model was identified as a particularly suitable technique, as it can flexibly capture both linear and nonlinear dependencies and effectively identify the most influential variables. This integrated approach of EMD, curve fitting, Hilbert transform, and regression analysis methods provides valuable insights into the quantitative impact of multiscale turbulent eddies on the overall performance of the JI-NPR system. These findings are expected to guide targeted optimization of the reactor design to enhance the denitrification efficiency, a crucial goal for the practical application of this wastewater treatment technology.