CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis

Abstract

Colletotrichum gloeosporioides is the main causal agent of anthracnose in various plant species. Determining the molecular mechanisms underlying the pathogenicity and fungicide resistance of C. gloeosporioides could help build new strategies for disease control. The major facilitator superfamily (MFS) has multiple roles in the transport of a diverse range of substrates. In the present study, an MFS protein CgMFS1 was characterized in C. gloeosporioides. This protein contains seven transmembrane domains, and its predicted 3D structure is highly similar to the reported hexose transporters. To investigate the biological functions of CgMFS1, the gene knock-out mutant ΔCgMFS1 was constructed. A colony growth assay showed that the mutant was remarkably decreased in vegetative growth in minimal medium supplemented with monosaccharides and oligosaccharides as the sole carbon sources, whereas it showed a similar growth rate and colony morphology as wild types when using soluble starch as the carbon source. A stress assay revealed that CgMFS1 is involved in oxidative stress but not in the fungicide resistance of C. gloeosporioides. Furthermore, its pathogenicity was significantly impaired in the mutant, although its appressorium formation was not affected. Our results demonstrate that CgMFS1 is required for sugar transport, resistance to oxidative stress, and the pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.

The histone deacetylase HOS2 controls pathogenicity through regulation of melanin biosynthesis and appressorium formation in Colletotrichum gloeosporioides

“…The results revealed that the tolerance of ∆CgHOS2 to oxidative, salt, and cell wall inhibitor stress was decreased compared with that of the WT. In addition, the expression levels of a series of MFS and ABC transporters, which are related to stress tolerance (Reddy et al 2012;Liu et al 2021), were decreased in ∆CgHOS2, and the expression of many cell wall synthesis-related enzymes was also decreased in the mutant. The decrease in the stress tolerance of ∆CgHOS2 might partly result from the repression of these genes.…”

“…The gene complementation and CgHOS2-sGFP expression plasmids were linearized before protoplast transformation. Protoplast preparation and transformation were carried out as described previously (Liu et al 2021). At 3-4 days after transformation, the transformants resistant to chlorimuron ethyl or hygromycin B were selected for further PCR detection.…”

Actin‐bundling protein fimbrin regulates pathogenicity via organizing F‐actin dynamics during appressorium development in Colletotrichum gloeosporioides

“…Vegetative growth of C. gloeosporioides was examined by culturing the strains on minimal medium. The stress tolerance of the strains was estimated according to our previous work (Liu, Wang, et al, 2021). The chemicals 10 and 50 mM H 2 O 2 , 0.7 M NaCl, 1 M sorbitol, 0.25 mg/L Congo red, 0.1 μg/ml fludioxonil, 10 μg/ml iprodione, 0.2 μg/ml tebuconazole, and 2.5 μg/ml TriadiMefon were used to simulate stresses.…”

Advances in understanding the mechanism of resistance to anthracnose and induced defence response in tea plants

“…In plant pathogens, transporters play an essential role in protection against plant defence mechanisms. One of the major classes of transporter is the major facilitator superfamily (MFS) transporter ( CgMfs1 ), which is very similar to hexose transporters required for sugar transport, resistance to oxidative stress, and the pathogenicity of C. gloeosporioides (Liu et al, 2021). C. gloeosporioides requires copper at the initial stages of pathogenesis and germination, and potassium for maintaining cell shape, membrane potential, intracellular pH, and enzyme activity (Barhoom et al, 2008; Pan et al, 2022).…”