CH4 oxidation-dependent 15N2 fixation in rice roots in a low-nitrogen paddy field and in Methylosinus sp. strain 3S-1 isolated from the roots

Shinoda, Ryo; Bao, Zhihua; Minamisawa, Kiwamu

doi:10.1016/j.soilbio.2019.01.021

Cited by 27 publications

(25 citation statements)

References 41 publications

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“…The diazotrophic potentials of the swamp soils share similar patterns with methanotrophic potentials, i.e., with the highest potentials in the intermediate soils, implying the dependence of nitrogen fixation on methane oxidation under suboxic conditions. This is in accordance with a previous study suggesting CH 4 oxidation-dependent N 2 fixation in a low-nitrogen paddy soil (Shinoda et al, 2019). Methanotrophic nitrogen fixation sustains the accumulation of bioavailable carbon and nitrogen in pristine peatlands (Vile et al, 2014) and oligotrophic peatlands (Larmola et al, 2014).…”

Section: Discussionsupporting

confidence: 93%

Active Methanotrophs in Suboxic Alpine Swamp Soils of the Qinghai–Tibetan Plateau

et al. 2020

Front. Microbiol.

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Section: Discussionsupporting

confidence: 93%

Active Methanotrophs in Suboxic Alpine Swamp Soils of the Qinghai–Tibetan Plateau

et al. 2020

Front. Microbiol.

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“…Recently, Kits et al (2015) have used complete genome analysis to show that the type I methanotrophs Methylomonas denitrificans FJG1 possess related denitrification genes and demonstrate denitrification activity under hypoxic conditions. In addition, Methylocystis and Methylosinus of type II methanotrophs were found to be the predominant root-associated methanotrophs in rice paddy field (Bao et al 2014b;Eller and Frenzel 2001;Qiu et al 2009;Shinoda et al 2019) and were identified as diazotrophic methanotrophs in rice root (Bao et al 2014b;Shinoda et al 2019). These results support that members of type I and type II methanotrophs inhabiting in aquatic plants in wetland.…”

Section: Discussionsupporting

confidence: 67%

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

et al. 2020

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Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.

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“…Recently, Kits et al (2015) have used complete genome analysis to show that the type I methanotrophs Methylomonas denitrificans FJG1 possess related denitrification genes and demonstrate denitrification activity under hypoxic conditions. In addition, Methylocystis and Methylosinus of type II methanotrophs were found to be the predominant root-associated activity bacteria in rice paddy field (Bao et al 2014b;Eller andFrenzel 2001 Qiu et al 2009;Shinoda et al 2019) and were identified as diazotrophic methanotrophs in rice root (Bao et al 2014b;Shinoda et al 2019). These results support that members of type I and type II methanotrophs inhabiting in aquatic plant in wetland.…”

Section: Discussionmentioning

confidence: 99%

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Chen

Zhao

Wang

et al. 2020

Preprint

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Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the vegetation type on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmo A gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis , Typha angustifolia , and Scirpus triqueter from a eutrophic wetland. High-throughput sequencing of pmo A based on DNA and cDNA was used to analyze the methanotrophic community . Sequencing of cDNA pmo A amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, M ethylomonas , was the most active group in P. australis , whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter . In T. angustifolia , these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.

show abstract

CH4 oxidation-dependent 15N2 fixation in rice roots in a low-nitrogen paddy field and in Methylosinus sp. strain 3S-1 isolated from the roots

Cited by 27 publications

References 41 publications

Active Methanotrophs in Suboxic Alpine Swamp Soils of the Qinghai–Tibetan Plateau

Active Methanotrophs in Suboxic Alpine Swamp Soils of the Qinghai–Tibetan Plateau

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

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