Chair side disinfection of gutta - percha points - An in vitro comparative study between 5 different agents at different concentrations

Pradeep, K; Kidiyoor, KH; Jain, Pavithra Amrath; Rao, Neha

doi:10.4103/0970-7212.352318

Endodontology

2013

DOI: 10.4103/0970-7212.352318

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Chair side disinfection of gutta - percha points - An in vitro comparative study between 5 different agents at different concentrations

K Pradeep

KH Kidiyoor²,

Pavithra Amrath Jain³

et al.

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Degree of Contamination of Gutta-Percha Points by Staphylococcus aureus (MRSA/MSSA) Strains

Teles,

Pina,

Cardoso

et al. 2024

IJMS

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Methicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.

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Degree of Contamination of Gutta-Percha Points by Staphylococcus aureus (MRSA/MSSA) Strains

Teles,

Pina,

Cardoso

et al. 2024

IJMS

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Comparación de desinfección de diferentes marcas de punta de gutapercha con hipoclorito de sodio

García¹,

Barba²,

García³

et al. 2020

Revista De La Asociación Dental Mexicana

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Evaluation and Comparison of Three Chemical Agents with a New Herbal Agent for Disinfection of Gutta-percha Cones: An in vitro Study

Charlie¹,

Dhanapal²,

Ummer³

et al. 2016

Conservative Dentistry and Endodontic Journal

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Aims: To evaluate the effectiveness of grape seed extract (GSE) as a gutta-percha disinfectant and to compare the efficacy with 5% sodium hypochlorite, 2.5% sodium hypochlorite, and 2% chlorhexidine against Bacillus subtilis.Materials and methods: Minimum inhibitory concentration and minimum bactericidal concentration of GSE were calculated by serial dilution and well-diffusion method. Five hundred gutta-percha cones of ISO size 25 were divided into 10 equal groups (n = 50). The 10 groups of gutta-percha cones were inoculated with cultured Bacillus species and incubated for 72 hours for allowing bacterial growth. Each group was then treated with the test solutions for 1 and 5 minutes. The treated groups of gutta-percha cones were then incubated in brain heart infusion agar allowing for bacterial growth, which were later analyzed by the turbidity of the medium. The results were statistically analyzed.Results: Gutta-percha cones when treated with 2% chlorhexidine gluconate for 1 and 5 minutes showed the most inhibiting activity against B. subtilis. Grape seed extract was found to have limited activity against B. subtilis in both 1-and 5-minute interval. Both concentrations of NaOCl, 2.5 and 5%, showed reduced activity against B. subtilis. Conclusion:Grape seed extract though has antibacterial activity, when used as gutta-percha disinfectant, was found to be less effective than chlorhexidine gluconate against B. subtilis.Clinical significance: Different methods of gutta-percha cone disinfection have been advocated and GSE for gutta-percha cone disinfection was attempted owing to its herbal antibacterial nature.

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Chair side disinfection of gutta - percha points - An in vitro comparative study between 5 different agents at different concentrations

Cited by 3 publications

References 0 publications

Degree of Contamination of Gutta-Percha Points by Staphylococcus aureus (MRSA/MSSA) Strains

Degree of Contamination of Gutta-Percha Points by Staphylococcus aureus (MRSA/MSSA) Strains

Comparación de desinfección de diferentes marcas de punta de gutapercha con hipoclorito de sodio

Evaluation and Comparison of Three Chemical Agents with a New Herbal Agent for Disinfection of Gutta-percha Cones: An in vitro Study

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