Chalcone synthase expression and pigments deposition in wheat with purple and blue colored caryopsis

Trojan, Václav; Musilová, M.; Vyhnánek, Tomáš; Klejdus, Bořivoj; Hanáček, Pavel; Havel, Ladislav

doi:10.1016/j.jcs.2013.10.008

Cited by 34 publications

(23 citation statements)

References 28 publications

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“…Accordingly, no MTA was found in that subspecies. Remarkably, the wPt-5839(2AS), whose putative related gene is a chalcone synthase (CHS), a key enzyme in the pigments deposition and color of wheat kernels [62], was monomorphic in dicoccon . In addition to SC, in the subsp.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon

Giraldo

Royo²,

González³

et al. 2016

PLoS ONE

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Association mapping was performed for 18 agromorphological and grain quality traits in a set of 183 Spanish landraces, including subspecies durum, turgidum and dicoccon, genotyped with 749 DArT (Diversity Array Technology) markers. Large genetic and phenotypic variability was detected, being the level of diversity among the chromosomes and genomes heterogeneous, and sometimes complementary, among subspecies. Overall, 356 were monomorphic in at least one subspecies, mainly in dicoccon, and some of them coincidental between subspecies, especially between turgidum and dicoccon. Several of those fixed markers were associated to plant responses to environmental stresses or linked to genes subjected to selection during tetraploid wheat domestication process. A total of 85 stable MTAs (marker–trait associations) have been identified for the agromorphological and quality parameters, some of them common among subspecies and others subspecies-specific. For all the traits, we have found MTAs explaining more than 10% of the phenotypic variation in any of the three subspecies. The number of MTAs on the B genome exceeded that on the A genome in subsp. durum, equalled in turgidum and was below in dicoccon. The validation of several adaptive and quality trait MTAs by combining the association mapping with an analysis of the signature of selection, identifying the putative gene function of the marker, or by coincidences with previous reports, showed that our approach was successful for the detection of MTAs and the high potential of the collection to identify marker–trait associations. Novel MTAs not previously reported, some of them subspecies specific, have been described and provide new information about the genetic control of complex traits.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon

Giraldo

Royo²,

González³

et al. 2016

PLoS ONE

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“…The regulatory genes for plant anthocyanin biosynthesis include genes encoding MYB, bHLH and WD40 transcription factors, which control anthocyanin accumulation in the tissues by regulating the expression of structural genes [36,37]. The expression of CHS and DFR in purple wheat showed tissue and cultivar specificity, and DFR had 3 copies; CHS in different cultivars contained a single nucleotide polymorphism [38,39]. The results of this study, together with those of other recent publications (Fig 4) [40,41], indicate that anthocyanins are produced in a highly regulated, fine-tuned, and nutrient-specific manner as a result of metabolic adaptation to nutrient stress.…”

Section: Plos Onementioning

confidence: 99%

Identification of colored wheat genotypes with suitable quality and yield traits in response to low nitrogen input

Fan

Wang

et al. 2020

PLoS ONE

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Colored wheat is a valuable resource that is rich in anthocyanins and minerals and thus contributes additional nutritional value to a healthy human diet. However, the effects of nitrogen fertilization on anthocyanin content (AC) and the balance between quality and yield still merit discussion. In this study, blue, purple, and common-colored wheat genotypes were used to investigate three nutrient quality traits, seven processing quality traits, three yield traits and seven grain morphology traits at three nitrogen levels in two years to excavate their possible plasticity under low-nitrogen stress and the tradeoffs among these traits. The highest AC was found in the blue genotypes followed by the purple genotypes. Analysis of variance (ANOVA) showed that AC could be significantly increased by reducing N application, especially in the purple genotypes. Therefore, growing colored wheat with low nitrogen input could allow efficient harvesting of grain with higher AC. However, the other nutrient quality traits and most processing quality traits were observed to decrease under low-nitrogen (LN) stress. Additionally, a correlation analysis indicated that the nutrient quality traits had stable tradeoffs with thousand kernel weight at all N levels because of the significantly negative correlations among them. Therefore, the additive main effect and multiplicative interaction (AMMI) model was used to further identify the most suitable colored genotypes with the best yield potential and also nutrient quality relative characteristics under LN stress. The blue lines Lanmai2999 and purple varieties Zhongkezinuomai 168 were found to be specifically adapted to LN stress with the highest AC values and showed stable performance in the other nutrient quality-and yield-related features. To further investigate the possible mechanism of anthocyanin accumulation in response to reduced N application, the expression of four genes (TaCHS, TaFDR, TaCHI and TaANS) involved in the anthocyanin synthesis pathway was evaluated. All four genes were downregulated under high nitrogen fertilizer application, indicating that anthocyanin synthesis in colored wheat might be inhibited by nitrogen fertilizer. Therefore, this research provided information for optimizing nitrogen fertilizer management in producing colored wheat and also demonstrated that it is efficient and economical to plant colored wheat genotypes in nitrogen-poor areas for use in

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“…Another group, aurones, is also common (WINKEL-SHIRLEY, 2001). The unusual colouration of wheat (Triticum aestivum L.) caryopses is caused by anthocyanins, particularly cyanidin 3-glucoside and delphinidin-3-glucoside, while in purple wheat cyanidin 3glucoside is the most abundant anthocyanin and in blue delphinidin-3-glucoside is more prevalent (ABDEL-AAL et al, 2003;HOSSEINIAN et al, 2008;TROJAN et al, 2014). The enzymes involved in the biosynthetic pathways leading to anthocyanin group formation are chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4reductase (DFR) and anthocyanidin synthase (ANS) (MOL et al, 1998;DAVIES and SCHWINN, 2006;MA et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Sequence analysis of flavanone 3-hydroxylase and dihydroflavonol 4-reductase genes in wheat with nonstandard coloured caryopses

et al. 2019

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The DNA sequences of chosen structural genes, flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR), encoding key enzymes from the flavonoid biosynthetic pathway, were studied in this paper. Sequences were gained using different approaches, i.e. direct sequencing from the PCR product for F3H and a cloning strategy for DFR. Five bread wheat (Triticum aestivum L.) genotypes with nonstandard coloured caryopses, purple, blue, and white, were used as plant material. The sequence variability was observed among tested genotypes.

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Chalcone synthase expression and pigments deposition in wheat with purple and blue colored caryopsis

Cited by 34 publications

References 28 publications

Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon

Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon

Identification of colored wheat genotypes with suitable quality and yield traits in response to low nitrogen input

Sequence analysis of flavanone 3-hydroxylase and dihydroflavonol 4-reductase genes in wheat with nonstandard coloured caryopses

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