Challenge of determining true outcome of congenital diaphragmatic hernia

Davis, Carl; Walker, Gregor

doi:10.1136/archdischild-2018-314820

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…As for the characterization with the involvement frequency of the defect to gender, greater expressiveness was obtained in males (71.43%), without a statistical significance standard (p=0.18) expected (p-value≤0.05). The data corroborates the characterization of live children with CDH from 28 pediatric surgical centers in the United Kingdom and Ireland, where 1.5 times more births of males with CDH were observed in relation to the females 8 .…”

Section: Discussionsupporting

confidence: 86%

Hérnia diafragmática Congênita: aspectos clínico-hospitalares em um hospital de referência materno-infantil na região Amazônica

et al. 2020

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RESUMO O objetivo deste estudo é analisar o perfil clínico-hospitalar de neonatos com o diagnóstico de hérnia diafragmática congênita (HDC), no período de 2008 a 2018 em um hospital de referência materno-infantil. Trata-se de um estudo transversal e analítico descritivo, que incluiu principalmente dados de prontuários com os códigos Q79.0, Q79.1, J986, K44 e K44.0 da Classificação Internacional de Doenças (CID-10). Os critérios de exclusão de prontuários foram o diagnóstico de outros tipos de hérnia diafragmática, sem o aspecto congênito; a internação em unidades que não fossem UTI Neonatal; e a idade no ato de internação igual ou superior a 29 dias. Analisou-se um total de 25.602 prontuários, dos quais 14 corresponderam a HDC. O acometimento por gênero foi de 71,43% masculino (10 casos) e 28,57% feminino (4), com 21,34% das localizações de HDC tipo Bochdalek à direita (3) e 78,57% à esquerda (11). Todos os neonatos deste estudo passaram por ventilação mecânica invasiva (VMI) 9,21 ± 5,55 dias. A cirurgia foi indicada em 11 casos (78,57%), com vias de acesso por toracotomia em 4 (36,36%) e por laparotomia subcostal em 7 (63,64%), todos com uso de dreno torácico homolateral ao hemitórax. O tempo total de internação foi de 19,42 ± 15,36 dias. Observou-se a evolução de alta melhorada em oito pacientes (57,14%) e o óbito de seis (42,86%), com idade de 6,19 ± 4,79 dias, sem referência de acompanhamento do desenvolvimento neuropsicomotor posteriormente. Ocorreram baixas incidências de casos por ano, e o gênero de acometimento, as malformações associadas e o tempo de VMI foram semelhantes a outras populações no mundo.

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Section: Discussionsupporting

confidence: 86%

Hérnia diafragmática Congênita: aspectos clínico-hospitalares em um hospital de referência materno-infantil na região Amazônica

et al. 2020

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“…Pulmonary hypoplasia (PH), a major complication of congenital diaphragmatic hernia (CDH), is the primary factor responsible for the poor prognosis of newborns with CDH [1][2][3] . With the advancement of prenatal diagnostic and surgery technology over the past few decades, the cure rate of newborns with mild to moderate CDH was significantly improved 4,5 .…”

Section: Introductionmentioning

confidence: 99%

The miR-130a-5p regulates the proliferation and apoptosis of pulmonary epithelial cells by targeting Foxa2 in lung branching morphogenesis

Li,

Liu,

Wei

2024

Preprint

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Background: In previous study, we confirmed that the miR-130a-5p/Foxa2 axis regulates fetal lung development in congenital diaphragmatic hernia (CDH). We also showed that miR-130a-5p and Foxa2 in lung tissues were localized to the epithelial cells. However, the biological functions of the miR-130a-5p/Foxa2 axis in pulmonary epithelial cells have yet to be fully elucidated. In the present study, we focused on the effect of the miR-130a-5p/Foxa2 axis on the proliferation and apoptosis of pulmonary epithelial cells and explored the mechanisms underlying these processes. Methods: We investigated the roles of miR-130a-5p and Foxa2 with regards to cell viability and apoptosis, by transfecting miR-130a-5p mimics/inhibitors, and Foxa2 overexpressed lentivirus/siRNA, into BEAS-2B cells. We also conducted the CCK-8 assay, EdU staining, and ﬂow cytometry. Chromatin Immunoprecipitation-sequencing (ChIP-Seq) and ChIP-PCR were applied to study the molecular mechanism of miR-130a-5p/Foxa2 axis affecting the the biological properties of pulmonary epithelial cells. Results: We found that the upregulation of miR-130a-5p suppressed cell proliferation and promoted apoptosis, while the downregulation of miR-130a-5p had the opposite effects. Upregulation of the direct target gene of miR-130a-5p, Foxa2, was able to rescue the effects of miR-130a-5p in Human bronchial epithelial (BEAS-2B) cells. Chip-seq and Chip-PCR assays identified potential transcription factor functions for Foxa2; Gli3 and Eya3 were identified as binding sites for Foxa2, and the potential mechanism required our further study. Conclusions: The results showed that miR-130a-5p/Foxa2 axis played an important role in the epigenetic modification of pulmonary epithelial cells, and our current ﬁndings provided a potential mechanism for lung branching morphogenesis.

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Challenge of determining true outcome of congenital diaphragmatic hernia

Cited by 2 publications

References 6 publications

Hérnia diafragmática Congênita: aspectos clínico-hospitalares em um hospital de referência materno-infantil na região Amazônica

Hérnia diafragmática Congênita: aspectos clínico-hospitalares em um hospital de referência materno-infantil na região Amazônica

The miR-130a-5p regulates the proliferation and apoptosis of pulmonary epithelial cells by targeting Foxa2 in lung branching morphogenesis

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