Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Ascensión, Alex M.; Araúzo-Bravo, Marcos J.; Izeta, Ander

doi:10.3390/life12010067

Cited by 7 publications

(4 citation statements)

References 127 publications

(171 reference statements)

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“…Although the new approach is enabled by the recent scRNA-seq technology, the limitations of the new technology should be carefully considered for translating the results into biologic understandings ( 61 ). Current single-cell technologies offer limited number of cells that can be processed at a time and a limited number of mRNA molecules can be captured per cell ( 62 , 63 ).…”

Section: Discussionmentioning

confidence: 99%

The imbalance between Type 17 T-cells and regulatory immune cell subsets in psoriasis vulgaris

2022

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Psoriasis vulgaris is a common inflammatory disease affecting 7.5 million adults just in the US. Previously, psoriasis immunopathogenesis has been viewed as the imbalance between CD4+ T-helper 17 (Th17) cells and regulatory T-cells (Tregs). However, current paradigms are rapidly evolving as new technologies to study immune cell subsets in the skin have been advanced. For example, recently minted single-cell RNA sequencing technology has provided the opportunity to compare highly differing transcriptomes of Type 17 T-cell (T17 cell) subsets depending on IL-17A vs. IL-17F expression. The expression of regulatory cytokines in T17 cell subsets provided evidence of T-cell plasticity between T17 cells and regulatory T-cells (Tregs) in humans. In addition to Tregs, other types of regulatory cells in the skin have been elucidated, including type 1 regulatory T-cells (Tr1 cells) and regulatory dendritic cells. More recently, investigators are attempting to apply single-cell technologies to clinical trials of biologics to test if monoclonal blockade of pathogenic T-cells will induce expansion of regulatory immune cell subsets involved in skin homeostasis.

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Section: Discussionmentioning

confidence: 99%

The imbalance between Type 17 T-cells and regulatory immune cell subsets in psoriasis vulgaris

2022

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“…It is currently being used extensively in the field of keloid research ( Onoufriadis et al, 2018 ; Matsumoto et al, 2020 ). Single-cell RNA sequencing (RNA-seq) allows the transcriptome level of a large number of cells to be studied at single-cell resolution and has been applied to the study of the mechanisms of keloid formation ( Xie et al, 2021a ; Ascensión et al, 2022 ). A recent study used the single cell transcriptome to identify four main subpopulations of keloid fibroblasts, secretory papillae, secretory reticulocytes, mesenchymal cells and pro-inflammatory cells.…”

Section: Introductionmentioning

confidence: 99%

Activation of the NFκB signaling pathway in IL6+CSF3+ vascular endothelial cells promotes the formation of keloids

Liu

Zhang

Zhen

et al. 2022

Front. Bioeng. Biotechnol.

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Background: Keloid is a disease caused by abnormal proliferation of skin fibres, the causative mechanism of which remains unclear.Method: In this study, endothelial cells of keloids were studied using scRNAseq combined with bulk-RNAseq data from keloids. The master regulators driving keloid development were identified by transcription factor enrichment analysis. The pattern of changes in vascular endothelial cells during keloid development was explored by inferring endothelial cell differentiation trajectories. Deconvolution of bulkRNAseq by CIBERSORTX verified the pattern of keloidogenesis. Immunohistochemistry for verification of the lesion process in keloid endothelial cells.Results: The endothelial cells of keloids consist of four main cell populations (MMP1+ Endo0, FOS + JUN + Endo1, IL6+CSF3+Endo2, CXCL12 + Endo3). Endo3 is an endothelial progenitor cell, Endo1 is an endothelial cell in the resting state, Endo2 is an endothelial cell in the activated state and Endo0 is an endothelial cell in the terminally differentiated state. Activation of the NFΚB signaling pathway is a typical feature of Endo2 and represents the early skin state of keloids.Conclusion: We have identified patterns of vascular endothelial cell lesions during keloidogenesis and development, and have found that activation of the NFΚB signaling pathway is an essential feature of keloid formation. These findings are expected to contribute to the understanding of the pathogenesis of keloids and to the development of new targeted therapeutic agents for the lesional characteristics of vascular endothelial cells.

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“…Biologically, this is mostly due to posttranslational modifications, RNA silencing or different half‐lives of proteins and RNA that affect cell physiology, and transcriptomics analyses are blind to them. A mechanistic understanding of both mRNA and protein is needed to interpret such data as best as possible [131]. The scarce nature of datasets and the massive amount of data make the computational aspect challenging.…”

Section: The Trade‐offs Of Single‐cell Sequencingmentioning

confidence: 99%

Applications of single‐cell omics for chimeric antigen receptor T cell therapy

Ghaffari,

Saleh,

Akbari

et al. 2023

Immunology

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Chimeric antigen receptor (CAR) T cell therapy is a promising cancer treatment modality. The breakthroughs in CAR T cell therapy were, in part, possible with the help of cell analysis methods, such as single‐cell analysis. Bulk analyses have provided invaluable information regarding the complex molecular dynamics of CAR T cells, but their results are an average of thousands of signals in CAR T or tumour cells. Since cancer is a heterogeneous disease where each minute detail of a subclone could change the outcome of the treatment, single‐cell analysis could prove to be a powerful instrument in deciphering the secrets of tumour microenvironment for cancer immunotherapy. With the recent studies in all aspects of adoptive cell therapy making use of single‐cell analysis, a comprehensive review of the recent preclinical and clinical findings in CAR T cell therapy was needed. Here, we categorized and summarized the key points of the studies in which single‐cell analysis provided insights into the genomics, epigenomics, transcriptomics and proteomics as well as their respective multi‐omics of CAR T cell therapy.

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Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to Dermatology

Cited by 7 publications

References 127 publications

The imbalance between Type 17 T-cells and regulatory immune cell subsets in psoriasis vulgaris

The imbalance between Type 17 T-cells and regulatory immune cell subsets in psoriasis vulgaris

Activation of the NFκB signaling pathway in IL6+CSF3+ vascular endothelial cells promotes the formation of keloids

Applications of single‐cell omics for chimeric antigen receptor T cell therapy

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