Challenges and opportunities of using immobilized lipase as biosensor

Iyer, M.K.; Shreshtha, Ishita; Baradia, Hrithik; Chattopadhyay, Soham

doi:10.1080/02648725.2022.2050499

Cited by 10 publications

(2 citation statements)

References 63 publications

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“…1 It has also attracted attention as the recognition element in electrochemical biosensors to detect analytes such as triglycerides, pesticides, and fungicides. [2][3][4][5] The use of an enzyme in biosensors is still limited due to its unstable nature and short lifetime. It is prone to denaturation, biofouling, and electrode passivation, resulting in low selectivity.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a lipase/reduced graphene oxide/metal–organic framework electrode using a central composite design-response surface methodology approach

2023

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Section: Introductionmentioning

confidence: 99%

Optimization of a lipase/reduced graphene oxide/metal–organic framework electrode using a central composite design-response surface methodology approach

2023

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“…Particularly in point-of-care testing (POCT) applications, immunoassay-based surface chemistry has emerged as a versatile and highly specific method [9], leveraging solid surfaces that are conjugated with target antibodies to facilitate detection [10][11][12][13][14][15]. To further enhance surface functionality for POCT [16], nanoparticles have been employed as solid matrices for antibody immobilization [17][18][19][20]. However, this approach presents challenges for deployment in resource-limited settings, where the immunoassay-based microfluidic chips are subject to degradation over time [21].…”

Section: Introductionmentioning

confidence: 99%

Extending the Shelf-Life of Immunoassay-Based Microfluidic Chips through Freeze-Drying Sublimation Techniques

Moon

2023

Sensors

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Point-of-care testing (POCT) platforms utilizing immunoassay-based microfluidic chips offer a robust and specific method for detecting target antibodies, demonstrating a wide range of applications in various medical and research settings. Despite their versatility and specificity, the adoption of these immunoassay chips in POCT has been limited by their short shelf-life in liquid environments, attributed to the degradation of immobilized antibodies. This technical limitation presents a barrier, particularly for resource-limited settings where long-term storage and functionality are critical. To address this challenge, we introduce a novel freeze-dry sublimation process aimed at extending the shelf-life of these microfluidic chips without compromising their functional integrity. This study elaborates on the mechanisms by which freeze-drying preserves the bioactivity of the immobilized antibodies, thereby maintaining the chip’s performance over an extended period. Our findings reveal significant shelf-life extension, making it possible for these POCT platforms to be more widely adopted and practically applied, especially in settings with limited resources. This research paves the way for more accessible, long-lasting, and effective POCT solutions, breaking down previous barriers to adoption and application.

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